CAJ | 학술논문

将纯化的黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CCMMV)制剂免疫BALB/c小鼠,最后一次免疫后第3天取其脾细胞与SP2/0细胞融合,经采用选择性培养基、有限稀释法克隆和间接ELISA方法进行筛选,成功获得了3株分泌CGMMV特异性单克隆抗体的杂交瘤细胞株并分别命名为3C9,3F7,2G10.用ELISA方法对所获得的3个杂交瘤细胞株进行亚型鉴定均为IgG2a,kappa链.间接ELISA效价测定结果分别为3C9:1.024×10~7,3F7:2.56×10~6,2G10:1.28×10~6.此3株杂交瘤细胞所分泌的单克隆抗体均能与本研究室保存的其他3种不同的CGMMV分离物发生特异性反应,而不与其他3种同属成员病毒-烟草花叶病毒(Tobacco mosaic virus,TMV)、辣椒轻斑驳病毒(Pepper mild mottle virus,PMMoV)、齿瓣兰环斑病毒(Odontoglossum ringspot virus,ORSV)发生反应.
장순화적황과록반박화협병독(Cucumber green mottle mosaic virus,CCMMV)제제면역BALB/c소서,최후일차면역후제3천취기비세포여SP2/0세포융합,경채용선택성배양기、유한희석법극륭화간접ELISA방법진행사선,성공획득료3주분비CGMMV특이성단극륭항체적잡교류세포주병분별명명위3C9,3F7,2G10.용ELISA방법대소획득적3개잡교류세포주진행아형감정균위IgG2a,kappa련.간접ELISA효개측정결과분별위3C9:1.024×10~7,3F7:2.56×10~6,2G10:1.28×10~6.차3주잡교류세포소분비적단극륭항체균능여본연구실보존적기타3충불동적CGMMV분리물발생특이성반응,이불여기타3충동속성원병독-연초화협병독(Tobacco mosaic virus,TMV)、랄초경반박병독(Pepper mild mottle virus,PMMoV)、치판란배반병독(Odontoglossum ringspot virus,ORSV)발생반응.
The Cucumber green mottle mosaic virus(CGMMV)was injected to BALB/c mice whose cells were fused with myeloma cells SP2/0 3 days after the final injection.On the selective medium,3 hybridoma cell lines were obtained by the finite dilution and indirect-ELISA.The 3 lines can secrete monoclonal antibody(McAbs)stably and were named as 3C9,3F7 and 2G10.respectively.The subtypes were identified by ELISA as IgG2a,Kappa light chain;the titration was examined,which were 3C9:1.024×10~7,3F7:2.56×10~6 and 2G10:1.28×10~6,respectively.By indirect-ELISA,3C9,3F7 and 2G10 were demonstrated to react specifically to the three different CGMMV isolates from other origins,whereas 3C9,3F7 and 2G10 did not response to either of the members of the same genus,TMV,PMMV or ORSV.