CAJ | 학술논문

通过共价固定方法将羟基红花黄色素A(HSYA)抗血清蛋白固定到多孔硅Bragg反射镜的孔洞中,定量分析了不同浓度的羟基红花黄色素A人工抗原与特异性抗羟A多克隆抗体反应后多孔硅Bragg反射镜的反射谱峰位的红移情况.对比研究了固定阴性血清蛋白的多孔硅Bragg反射镜基底在加入抗原后的反射谱峰位变化情况,结果表明,基于多孔硅Bragg反射镜的光学免疫检测具有很好的特异性,且同目前普遍使用的ELISA方法相比,具有免标记且检测时间短等优异性能,同时该研究也为开发红花成分快速检测的免标记多孔硅生物传感器奠定了基础.
통과공개고정방법장간기홍화황색소A(HSYA)항혈청단백고정도다공규Bragg반사경적공동중,정량분석료불동농도적간기홍화황색소A인공항원여특이성항간A다극륭항체반응후다공규Bragg반사경적반사보봉위적홍이정황.대비연구료고정음성혈청단백적다공규Bragg반사경기저재가입항원후적반사보봉위변화정황,결과표명,기우다공규Bragg반사경적광학면역검측구유흔호적특이성,차동목전보편사용적ELISA방법상비,구유면표기차검측시간단등우이성능,동시해연구야위개발홍화성분쾌속검측적면표기다공규생물전감기전정료기출.
Porous silicon has attracted considerable attention for sensor application due to its remarkable optical properties and the easy fabrication. In this study, an optical immunoassay was developed by the optic reflectance spectroscopy of the porous silicon Bragg reflectors. The artificial immunogen of hydroxysafflor yellow A(HSYA-BSA) and the polyelonal anti-HSYA antibodies were employed as the target and probe, respectively. HSYA is the main chemical component of the flower of the safflower, which is widely used in traditional Chinese medicine for treatment of eerebrovascular and cardiovascular diseases. First, porous silicon Bragg reflector was functionalized using oxidation and silanization. Then, the polyclonal anti-HSYA antibodies were immobilized to the porous silicon Bragg reflector surface used a crosslink method. After the antigen-antibody reaction, the reflection spectrum red shifts and the red shift of spectrum increases with the increase of HSYABSA concentration. It is showing the red shifts of the reflectance spectrum as function of HSYA-BSA concentrations. Control experiments were also presented with negative serum and the result suggests that the reflection spectrum red shift is due to selective antigen-antibody binding and this porous silicon Bragg reflector immunosensor possess high specificity. Compared to conventional ELISA, the procedures are label-free and detection time is reduced. Thus, this research lays a foundation of exploiting label-free porous silicon Bragg reflector immunosensor and could be further developed for component detection of safflower of a quick and accurate determination.