大豆科学
大豆科學
대두과학
SOYBEAN SCIENCE
2010年
1期
109-112
,共4页
王玲琴%王志耕%方玉明%赵伟华%陈美姣%马丽丽
王玲琴%王誌耕%方玉明%趙偉華%陳美姣%馬麗麗
왕령금%왕지경%방옥명%조위화%진미교%마려려
大豆分离蛋白%水解度%多肽%降胆固醇
大豆分離蛋白%水解度%多肽%降膽固醇
대두분리단백%수해도%다태%강담고순
Soy protein isolate%Degree of hydrolysis%Peptides%Hypocholesterolemic activity
通过比较4种酶对大豆分离蛋白的水解效果,并通过单因素及L_9(3~4)正交试验优化其水解工艺条件,研究其最佳水解工具酶及最佳酶解参数.结果表明:木瓜蛋白酶和植物蛋白酶联合应用可作为大豆分离蛋白的水解工具酶;其最佳酶解参数为:酶解温度55℃、初始pH7.0、底物浓度12%、酶添加量8%、植物蛋白酶与木瓜蛋白酶的质量之比为1: 2,水解度可达14.20%;用双蛋白酶水解大豆分离蛋白,水解度为14.71%的产物降胆固醇活性最高,对胆固醇胶束溶解度的抑制率为61.67%.
通過比較4種酶對大豆分離蛋白的水解效果,併通過單因素及L_9(3~4)正交試驗優化其水解工藝條件,研究其最佳水解工具酶及最佳酶解參數.結果錶明:木瓜蛋白酶和植物蛋白酶聯閤應用可作為大豆分離蛋白的水解工具酶;其最佳酶解參數為:酶解溫度55℃、初始pH7.0、底物濃度12%、酶添加量8%、植物蛋白酶與木瓜蛋白酶的質量之比為1: 2,水解度可達14.20%;用雙蛋白酶水解大豆分離蛋白,水解度為14.71%的產物降膽固醇活性最高,對膽固醇膠束溶解度的抑製率為61.67%.
통과비교4충매대대두분리단백적수해효과,병통과단인소급L_9(3~4)정교시험우화기수해공예조건,연구기최가수해공구매급최가매해삼수.결과표명:목과단백매화식물단백매연합응용가작위대두분리단백적수해공구매;기최가매해삼수위:매해온도55℃、초시pH7.0、저물농도12%、매첨가량8%、식물단백매여목과단백매적질량지비위1: 2,수해도가체14.20%;용쌍단백매수해대두분리단백,수해도위14.71%적산물강담고순활성최고,대담고순효속용해도적억제솔위61.67%.
Plant protease and papain were regarded as the compound enzyme of soy protein isolate(SPI)in comparison with hydrolysis effects of four kinds of protease.The premium working conditions for enzymolysis were determined by means of single factor and L_9(3~4)orthogonal experiments.The optimal enzymic hydrolysis parameters were as followes:hydrolysis temperature55℃,initial pH 7.0,substrate concentration 12%,proteinase quantity 8%,the mass ratio of plant protease to papain 1: 2.Under the optimized conditions,the hydrolysis rate reached 14.2%.The hypocholesterolemic activity of the hydrolysate of SPI with 8.27 %～16.57 %DH prepared by two protease was studied.The results indicated that the sample prepared by hydrolyzing SPI with two protease at DH=14.71% shown highest hypocholesterolemic activity among all the samples,the inhibitory ratio of micellar solubility of cholesterol was 61.67% .
