中国介入影像与治疗学
中國介入影像與治療學
중국개입영상여치료학
CHINESE JOURNAL OF INTERVENTIONAL IMAGING AND THERAPY
2010年
2期
192-195
,共4页
兔%肝脏%VX2%模型,动物%艾迪注射液
兔%肝髒%VX2%模型,動物%艾迪註射液
토%간장%VX2%모형,동물%애적주사액
Rabbits%Liver%VX2%Models,animal%AiDi injection
目的 探讨超声引导下复方中药艾迪注射液瘤内注射治疗兔移植性VX2肝肿瘤的作用效果及机制.方法 建立新西兰大白兔移植性肝VX2肿瘤模型28只,随机分为3组,即艾迪注射液组(12只)、无水乙醇组(PEI组,8只)、生理盐水组(8只).全部动物在接种2周后每隔3天向肿瘤内注入治疗药物,注射4次后处死动物.于术前术后兔耳缘静脉抽血行血生化检查,同时行64排螺旋CT扫描测量并计算肿瘤体积、肿瘤增长率及肿瘤坏死率,以及进行组织细胞学与细胞超微结构的观察.结果 各实验组病灶均进行性增大,但艾迪注射液组肿瘤增长率及坏死率最小,与生理盐水组比较差异均有统计学意义(P＜0.05),与无水乙醇组比较差异无统计学意义;TUNEL法检测显示艾迪注射液组凋亡指数明显高于无水乙醇组及生理盐水组(P＜0.05);艾迪注射液组肝肾功能及白细胞计数治疗前后均无明显差异,组内及组间比较差异无统计学意义(P＞0.05).结论 艾迪注射液可诱导肿瘤细胞凋亡,对肿瘤细胞具有细胞毒性作用.
目的 探討超聲引導下複方中藥艾迪註射液瘤內註射治療兔移植性VX2肝腫瘤的作用效果及機製.方法 建立新西蘭大白兔移植性肝VX2腫瘤模型28隻,隨機分為3組,即艾迪註射液組(12隻)、無水乙醇組(PEI組,8隻)、生理鹽水組(8隻).全部動物在接種2週後每隔3天嚮腫瘤內註入治療藥物,註射4次後處死動物.于術前術後兔耳緣靜脈抽血行血生化檢查,同時行64排螺鏇CT掃描測量併計算腫瘤體積、腫瘤增長率及腫瘤壞死率,以及進行組織細胞學與細胞超微結構的觀察.結果 各實驗組病竈均進行性增大,但艾迪註射液組腫瘤增長率及壞死率最小,與生理鹽水組比較差異均有統計學意義(P＜0.05),與無水乙醇組比較差異無統計學意義;TUNEL法檢測顯示艾迪註射液組凋亡指數明顯高于無水乙醇組及生理鹽水組(P＜0.05);艾迪註射液組肝腎功能及白細胞計數治療前後均無明顯差異,組內及組間比較差異無統計學意義(P＞0.05).結論 艾迪註射液可誘導腫瘤細胞凋亡,對腫瘤細胞具有細胞毒性作用.
목적 탐토초성인도하복방중약애적주사액류내주사치료토이식성VX2간종류적작용효과급궤제.방법 건립신서란대백토이식성간VX2종류모형28지,수궤분위3조,즉애적주사액조(12지)、무수을순조(PEI조,8지)、생리염수조(8지).전부동물재접충2주후매격3천향종류내주입치료약물,주사4차후처사동물.우술전술후토이연정맥추혈행혈생화검사,동시행64배라선CT소묘측량병계산종류체적、종류증장솔급종류배사솔,이급진행조직세포학여세포초미결구적관찰.결과 각실험조병조균진행성증대,단애적주사액조종류증장솔급배사솔최소,여생리염수조비교차이균유통계학의의(P＜0.05),여무수을순조비교차이무통계학의의;TUNEL법검측현시애적주사액조조망지수명현고우무수을순조급생리염수조(P＜0.05);애적주사액조간신공능급백세포계수치료전후균무명현차이,조내급조간비교차이무통계학의의(P＞0.05).결론 애적주사액가유도종류세포조망,대종류세포구유세포독성작용.
Objective To explore the principle,effect and method of percutaneous AiDi injection for treatment of implanted VX2 liver tumor with ultrasound guidance.Methods Twenty-eight New Zealand white rabbits with implanted VX2 liver tumors were randomly divided into 3 groups:AiDi injection group (n=12) ,percutaneous ethanol injection (PEI) group (n=8) ,and physiological saline group (n=8) .Two weeks after transplantation,the drugs were injected into tumors of all the rabbits once every 3 days.After 4 times injection,the rabbits were killed.The tumors were measured with 64-slice CT,while the rate of tumor necrosis,the volume of tumor and the growth rate of the tumor were calculated.Liver and kidney function tests (AST,ALT,ALP and BUN) as well as white blood eell counts were assessed before and after treatment,and CT examination was performed at the same time.The morphological and the ultramicrostructural changes were also investigated under light microscope or electron microscope.Results Tumors in all groups kept growing after the procedure.Among all 3 groups,tumor growing rate for AiDi injection group was the lowest and the necrotic rate was the highest (P＜0.05) ,while no significant difference was found between AiDi injection group and PEI group (P＞0.05) .Apoptotic index in AiDi injection group was significantly higher than that in PEI group and physiological saline group (P＜0.05) .There was no evident change of liver and kidney function before and after treatment in AiDi injection group and PEI group (P＞0.05) .Conclusion AiDi injection can induce apoptosis of liver VX2 tumor cells.Percutaneous AiDi injection therapy can obviously inhibit and destroy liver VX2 tumor cells.
