中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2012年
7期
531-535
,共5页
食物过敏%肺%小鼠%黄褐毛忍冬总皂苷
食物過敏%肺%小鼠%黃褐毛忍鼕總皂苷
식물과민%폐%소서%황갈모인동총조감
Food hypersensitivity%Lung%Mice%Fulvotomentoside
目的 观察卵清蛋白(OVA)过敏小鼠肺部免疫状态变化及黄褐毛忍冬总皂苷(Ful)的干预作用.方法 BALB/e雌鼠96只均分为6组(每组16只):1组和2组均经OVA腹腔注射致敏及灌胃激发,之后分别给予磷酸盐缓冲液(PBS)和OVA雾化吸入；3组及4组给予Ful( 200 mg/kg)皮下注射治疗共22 d,其余处理分别同1组和2组；5组未经OVA灌胃激发,其余处理同2组；6组为对照组.瑞氏-姬姆萨复合染色观察支气管肺泡灌洗液(BALF)中白细胞数及分类；肺组织HE染色进行气道炎症病理分析；免疫组织化学法观察肺组织中转化生长因子β1 (TGF-β1)、白细胞介素6( IL-6)、白细胞介素17A(IL-117A)蛋白表达水平；检测肺组织中髓过氧化物酶活性代表中性粒细胞活化水平.结果 1组肺部无明显炎细胞浸润；与6组比较,2组BALF中性粒细胞、淋巴细胞百分比显著增高,镜下可见大量红细胞,肺组织炎症病理评分以及肺组织中TGF-β1[ (75 437±3 638)vs.(6 118 ±1 978)]、IL-6[( 121 650±25 389)vs.(15 726±9 360)]、IL-17A[( 252 105±31 651)vs.(72 644±12 285)]表达水平均明显增高.5组肺组织炎症病理评分低于2组,且肺部以嗜酸粒细胞浸润为主,肺部TGF-β1(11 054±1 468)、IL-6(50 877±11 744)、IL-17A( 137 864±28 986)表达低于2组.Ful干预后吸入OVA的过敏小鼠肺部TGF-β1表达水平无明显改变,IL-6、IL-17A表达降低,但尚未能有效减轻中性粒细胞在局部的聚集及改善肺部炎症反应.结论 OVA过敏小鼠吸入特异性抗原后,可导致肺组织中TGF-β1、IL-6、IL-17A表达明显增高,引起严重的以中性粒细胞浸润为主的炎症.黄褐毛忍冬总皂苷可在一定程度上降低该小鼠肺组织中炎症因子1L-6、IL-17A的表达,但尚未能明显改善肺部病理状态.
目的 觀察卵清蛋白(OVA)過敏小鼠肺部免疫狀態變化及黃褐毛忍鼕總皂苷(Ful)的榦預作用.方法 BALB/e雌鼠96隻均分為6組(每組16隻):1組和2組均經OVA腹腔註射緻敏及灌胃激髮,之後分彆給予燐痠鹽緩遲液(PBS)和OVA霧化吸入；3組及4組給予Ful( 200 mg/kg)皮下註射治療共22 d,其餘處理分彆同1組和2組；5組未經OVA灌胃激髮,其餘處理同2組；6組為對照組.瑞氏-姬姆薩複閤染色觀察支氣管肺泡灌洗液(BALF)中白細胞數及分類；肺組織HE染色進行氣道炎癥病理分析；免疫組織化學法觀察肺組織中轉化生長因子β1 (TGF-β1)、白細胞介素6( IL-6)、白細胞介素17A(IL-117A)蛋白錶達水平；檢測肺組織中髓過氧化物酶活性代錶中性粒細胞活化水平.結果 1組肺部無明顯炎細胞浸潤；與6組比較,2組BALF中性粒細胞、淋巴細胞百分比顯著增高,鏡下可見大量紅細胞,肺組織炎癥病理評分以及肺組織中TGF-β1[ (75 437±3 638)vs.(6 118 ±1 978)]、IL-6[( 121 650±25 389)vs.(15 726±9 360)]、IL-17A[( 252 105±31 651)vs.(72 644±12 285)]錶達水平均明顯增高.5組肺組織炎癥病理評分低于2組,且肺部以嗜痠粒細胞浸潤為主,肺部TGF-β1(11 054±1 468)、IL-6(50 877±11 744)、IL-17A( 137 864±28 986)錶達低于2組.Ful榦預後吸入OVA的過敏小鼠肺部TGF-β1錶達水平無明顯改變,IL-6、IL-17A錶達降低,但尚未能有效減輕中性粒細胞在跼部的聚集及改善肺部炎癥反應.結論 OVA過敏小鼠吸入特異性抗原後,可導緻肺組織中TGF-β1、IL-6、IL-17A錶達明顯增高,引起嚴重的以中性粒細胞浸潤為主的炎癥.黃褐毛忍鼕總皂苷可在一定程度上降低該小鼠肺組織中炎癥因子1L-6、IL-17A的錶達,但尚未能明顯改善肺部病理狀態.
목적 관찰란청단백(OVA)과민소서폐부면역상태변화급황갈모인동총조감(Ful)적간예작용.방법 BALB/e자서96지균분위6조(매조16지):1조화2조균경OVA복강주사치민급관위격발,지후분별급여린산염완충액(PBS)화OVA무화흡입；3조급4조급여Ful( 200 mg/kg)피하주사치료공22 d,기여처리분별동1조화2조；5조미경OVA관위격발,기여처리동2조；6조위대조조.서씨-희모살복합염색관찰지기관폐포관세액(BALF)중백세포수급분류；폐조직HE염색진행기도염증병리분석；면역조직화학법관찰폐조직중전화생장인자β1 (TGF-β1)、백세포개소6( IL-6)、백세포개소17A(IL-117A)단백표체수평；검측폐조직중수과양화물매활성대표중성립세포활화수평.결과 1조폐부무명현염세포침윤；여6조비교,2조BALF중성립세포、림파세포백분비현저증고,경하가견대량홍세포,폐조직염증병리평분이급폐조직중TGF-β1[ (75 437±3 638)vs.(6 118 ±1 978)]、IL-6[( 121 650±25 389)vs.(15 726±9 360)]、IL-17A[( 252 105±31 651)vs.(72 644±12 285)]표체수평균명현증고.5조폐조직염증병리평분저우2조,차폐부이기산립세포침윤위주,폐부TGF-β1(11 054±1 468)、IL-6(50 877±11 744)、IL-17A( 137 864±28 986)표체저우2조.Ful간예후흡입OVA적과민소서폐부TGF-β1표체수평무명현개변,IL-6、IL-17A표체강저,단상미능유효감경중성립세포재국부적취집급개선폐부염증반응.결론 OVA과민소서흡입특이성항원후,가도치폐조직중TGF-β1、IL-6、IL-17A표체명현증고,인기엄중적이중성립세포침윤위주적염증.황갈모인동총조감가재일정정도상강저해소서폐조직중염증인자1L-6、IL-17A적표체,단상미능명현개선폐부병리상태.
Objective To investigate immune state in lung of BALB/c mice with ovalbumin (OVA) allergy and the effects of fulvotomentoside ( Ful ) on lungs of the mice and provide some clues for the mechanism that patients with food allergies were prone to asthma and observe the effects of the treatment with traditional Chinese medicine.Method Ninety-six female BALB/c mice were randomly divided into 6 groups.Mice in group 1 and group 2 were sensitized intraperitoneally and challenged intragastrically with OVA and were exposed to phosphate buffer solution and OVA respectively by nebulized inhalation.Mice in group 3 and group 4 were treated with Ful,other processes were the same as the mice in group 1 and group 2,respectively.Mice in group 5 were not challenged intragastrically with OVA and other processes were the same as the mice in group 2.Group 6 was the control group.The number of total leukocytes and cell classification in bronchoalveolar lavage (BALF) were counted,and inflammatory characteristic of lung was scored by staining with hematoxylin and eosin.The protein expressions of transforming growth factor ( TGF-β1),interleukin-6 (IL-6),interleukin-17 (IL-17A) in lung of the mice were detected by immunohistochemical method.The activation of neutrophils in lung was assayed by the level of myeloroxidase (MPO).Result There was no inflammatory cells infiltration in lung of the mice in group 1.Compared with group 6,numbers of total leukocytes and erythrocytes as well as the percentage of neutrophils and lymphocytes were increased in group 2.Inflammatory score and protein expressions of TGF-β1 [ (75 437 ±3 638)vs.(6 118 ± 1 978) ],IL-6 [ ( 121 650 ± 25 389) vs.( 15 726 ± 9 360) ],IL-17A [ (252 105 ±31 651 )vs.(72 644 ± 12 285 ) ] in lung were increased,too.Inflammatory score and TGF-β1 ( 11 054 ±1 468),IL-6 (50 877 ± 11 744 ),IL-17 A ( 137 864 ± 28 986 ) expressions in group 5 were lower than those in group 2. Eosinophils infiltration was significant in group 5. After the treatment with Ful,TGF-β1 expression did not change and IL-6,IL-17A expressions were decreased in lung of the mice that inhaled OVA.It was not enough for Ful to relieve the neutrophil aggregation and improve inflammatory reaction in lung.Conclusion The expressions of TGF-β1,IL-6,IL-17A in lung of the mice with OVA allergy were increased markedly after they inhaled specific antigen,which caused serious inflammation that was induced by neutrophil infiltration in lung.Ful could decrease the expressions of IL-6,IL-17A to some extent,but it was not enough to improve pathologic state in lung.
