中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
4期
332-334
,共3页
陈积贤%张洁%任振华%薛迪新%吴伟力%张仁虎%余铭%林道浙%林肖%黄建武%梁美珍%贺先伟
陳積賢%張潔%任振華%薛迪新%吳偉力%張仁虎%餘銘%林道浙%林肖%黃建武%樑美珍%賀先偉
진적현%장길%임진화%설적신%오위력%장인호%여명%림도절%림초%황건무%량미진%하선위
结直肠肿瘤%DNA甲基化%病理学,临床%p27基因
結直腸腫瘤%DNA甲基化%病理學,臨床%p27基因
결직장종류%DNA갑기화%병이학,림상%p27기인
Colorectal neoplasms%DNA methylation%Pathology,clinical%p27 gene
目的 检测结直肠癌组织中p27基因启动子区CpG岛甲基化水平及其表达,并结合其临床病理参数进行分析,探讨其在结直肠癌发生发展中的作用.方法 应用甲基化特异性聚合酶链反应分析技术检测106例结直肠癌组织及其正常结直肠黏膜组织、22例结肠腺瘤组织中p27基因启动子甲基化,用免疫组织化学SP方法检测其蛋白表达.结果 本组结直肠癌组织中p27甲基化阳性率为59.4%(63/106),结肠腺瘤组织中为18.2%(4/22),而正常结直肠黏膜组织中为3.8%(4/106),前组与后两组之间相比差异有统计学意义(P＜0.05).低分化组结直肠癌组织中p27甲基化阳性率明显高于高中分化组(48.0%比24.7%,P＜0.05);Dukes-A+B期组与C+D期组之间相比差异有统计学意义(20.0%比41.2%,P＜0.05);有无淋巴结转移两组之间的阳性率相比差异有统计学意义(41.5%比23.1%,P＜0.05);浸润深达浆膜层的结直肠癌组织中的甲基化阳性率与未达浆膜层组相比差异无统计学意义(32.5%比24.1%,P＞0.05).结论 结直肠癌组织中存在p27基因启动子甲基化.p27基因的高甲基化与结直肠癌分化程度、浸润深度、Dukes分期及有无淋巴结转移有关.
目的 檢測結直腸癌組織中p27基因啟動子區CpG島甲基化水平及其錶達,併結閤其臨床病理參數進行分析,探討其在結直腸癌髮生髮展中的作用.方法 應用甲基化特異性聚閤酶鏈反應分析技術檢測106例結直腸癌組織及其正常結直腸黏膜組織、22例結腸腺瘤組織中p27基因啟動子甲基化,用免疫組織化學SP方法檢測其蛋白錶達.結果 本組結直腸癌組織中p27甲基化暘性率為59.4%(63/106),結腸腺瘤組織中為18.2%(4/22),而正常結直腸黏膜組織中為3.8%(4/106),前組與後兩組之間相比差異有統計學意義(P＜0.05).低分化組結直腸癌組織中p27甲基化暘性率明顯高于高中分化組(48.0%比24.7%,P＜0.05);Dukes-A+B期組與C+D期組之間相比差異有統計學意義(20.0%比41.2%,P＜0.05);有無淋巴結轉移兩組之間的暘性率相比差異有統計學意義(41.5%比23.1%,P＜0.05);浸潤深達漿膜層的結直腸癌組織中的甲基化暘性率與未達漿膜層組相比差異無統計學意義(32.5%比24.1%,P＞0.05).結論 結直腸癌組織中存在p27基因啟動子甲基化.p27基因的高甲基化與結直腸癌分化程度、浸潤深度、Dukes分期及有無淋巴結轉移有關.
목적 검측결직장암조직중p27기인계동자구CpG도갑기화수평급기표체,병결합기림상병리삼수진행분석,탐토기재결직장암발생발전중적작용.방법 응용갑기화특이성취합매련반응분석기술검측106례결직장암조직급기정상결직장점막조직、22례결장선류조직중p27기인계동자갑기화,용면역조직화학SP방법검측기단백표체.결과 본조결직장암조직중p27갑기화양성솔위59.4%(63/106),결장선류조직중위18.2%(4/22),이정상결직장점막조직중위3.8%(4/106),전조여후량조지간상비차이유통계학의의(P＜0.05).저분화조결직장암조직중p27갑기화양성솔명현고우고중분화조(48.0%비24.7%,P＜0.05);Dukes-A+B기조여C+D기조지간상비차이유통계학의의(20.0%비41.2%,P＜0.05);유무림파결전이량조지간적양성솔상비차이유통계학의의(41.5%비23.1%,P＜0.05);침윤심체장막층적결직장암조직중적갑기화양성솔여미체장막층조상비차이무통계학의의(32.5%비24.1%,P＞0.05).결론 결직장암조직중존재p27기인계동자갑기화.p27기인적고갑기화여결직장암분화정도、침윤심도、Dukes분기급유무림파결전이유관.
Objective To investigate the relationship between p27 gene methylation and pathology of colorectal carcinoma. Methods p27 gene methylation promotor region and p27 protein expression were detected respectively by methylation specificity polymerase chain reaction and immunohistochemical staining SP in 106 cases of colorectal carcinoma and each adjacent normal mucous membrane tissue and 22 cases of colorectal adenoma tissue. Results The positive expression rate of p27 gene methylation was statistically different in colorectal carcinoma tissue compared with normal mucous membrane and colorectal adenoma tissue (P＜0.05). Their positive expression rate were 59.4% (63/106), 18.2% (4/22) and 3.8%(4/106) respectively in colorectal carcinoma tissue,colorectal adenoma and normal mucous membrane tissue (P ＜ 0. 05). p27 gene methylation in poorly differentiated group was significantly higher than that in welldifferentiated group (48.0% vs. 24. 7%, P ＜0. 05), in Dukes-A + B stage group was significantly lower than that in Dukes C + D stage group(20. 0% vs. 41.2%, P ＜ 0. 05 ), and it was higher in lymph nodes metastases group than that in lymph nodes negative group(41.5% vs. 23. 1%, P ＜0. 05), that in positive serosa infiltration group was higher than negative serosa infiltration group(32. 5% vs. 24. 1%, P ＞ 0. 05 ).Conclusions Methylated p27 gene protein expression in colorectal carcinoma was significantly higher than normal mucous membrane and colorectal adenoma tissue. The methylation rate of p27 gene in colorectal carcinoma was significantly associated with tumor differentiation, invasive depth, Dukes stage, lymph node metastasis.
