中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
8期
836-840
,共5页
杨绍敏%李惠琴%陈立力%李林%刘永健%钟敏%李健健%杨壁珲%高丽%樊移山%李敬云
楊紹敏%李惠琴%陳立力%李林%劉永健%鐘敏%李健健%楊壁琿%高麗%樊移山%李敬雲
양소민%리혜금%진립력%리림%류영건%종민%리건건%양벽혼%고려%번이산%리경운
艾滋病毒1型%基因型%分子流行病学
艾滋病毒1型%基因型%分子流行病學
애자병독1형%기인형%분자류행병학
Human immunodeficiency virus type 1%Genetic subtype%Molecular epidemiology
目的 了解云南省目前HIV-1流行株的基因型及其地区和人群分布.方法 收集2008-2009年云南省15个地市788例HIV感染者/艾滋病患者(HIV/AIDS)的血浆标本和背景信息,采用RT-PCR法分别扩增HIV-1 gag、pol全长基因(1584 bp和3147 bp)及env基因的C2V3片段(558 bp),序列编辑后用Genotyping及Mega 5.03软件工具确定病毒基因型,分析HIV-1株的地区和人群分布特征.结果 788例标本获得1728条HIV-1基因序列,其中全长gag基因序列599条、全长pol基因序列564条、env基因C2V3区序列525条,确定617例基因亚型,构成比分别为CRF08 BC(50.2％)、CRF01 AE(25.0％)、未知重组(10.2％)、CRF07_BC(9.2％)、C亚型(2.9％)和B(B')亚型(2.4％).HIV-1株在云南省具有地域分布特征,可分为以临沧和昆明为代表的CRF08_BC为主的地区,以德宏和西双版纳为代表的CRF08 _BC与CRF01 _AE构成比相近的地区；未知重组型在云南省少数民族中所占比例(17.0％)显著高于汉族(6.7％)；异性性传播感染者和静脉注射吸毒感染者的CRF08 BC亚型均占总数的50.0％以上,但前者CRF01_AE的构成比占约30.0％,后者未知重组型和CRF07 BC的比例分别达到约15.0％.未知重组病毒株呈现两种重组模式,分别为B(B')/C重组和以CRF01 AE为母株嵌入B(B ')和/或C片段重组,并以前者为主(74.6％).结论 云南省HIV-1株组成复杂,具有显著的地域、民族和传播途径相关的特征,并出现新型重组病毒株,应密切监测.
目的 瞭解雲南省目前HIV-1流行株的基因型及其地區和人群分佈.方法 收集2008-2009年雲南省15箇地市788例HIV感染者/艾滋病患者(HIV/AIDS)的血漿標本和揹景信息,採用RT-PCR法分彆擴增HIV-1 gag、pol全長基因(1584 bp和3147 bp)及env基因的C2V3片段(558 bp),序列編輯後用Genotyping及Mega 5.03軟件工具確定病毒基因型,分析HIV-1株的地區和人群分佈特徵.結果 788例標本穫得1728條HIV-1基因序列,其中全長gag基因序列599條、全長pol基因序列564條、env基因C2V3區序列525條,確定617例基因亞型,構成比分彆為CRF08 BC(50.2％)、CRF01 AE(25.0％)、未知重組(10.2％)、CRF07_BC(9.2％)、C亞型(2.9％)和B(B')亞型(2.4％).HIV-1株在雲南省具有地域分佈特徵,可分為以臨滄和昆明為代錶的CRF08_BC為主的地區,以德宏和西雙版納為代錶的CRF08 _BC與CRF01 _AE構成比相近的地區；未知重組型在雲南省少數民族中所佔比例(17.0％)顯著高于漢族(6.7％)；異性性傳播感染者和靜脈註射吸毒感染者的CRF08 BC亞型均佔總數的50.0％以上,但前者CRF01_AE的構成比佔約30.0％,後者未知重組型和CRF07 BC的比例分彆達到約15.0％.未知重組病毒株呈現兩種重組模式,分彆為B(B')/C重組和以CRF01 AE為母株嵌入B(B ')和/或C片段重組,併以前者為主(74.6％).結論 雲南省HIV-1株組成複雜,具有顯著的地域、民族和傳播途徑相關的特徵,併齣現新型重組病毒株,應密切鑑測.
목적 료해운남성목전HIV-1류행주적기인형급기지구화인군분포.방법 수집2008-2009년운남성15개지시788례HIV감염자/애자병환자(HIV/AIDS)적혈장표본화배경신식,채용RT-PCR법분별확증HIV-1 gag、pol전장기인(1584 bp화3147 bp)급env기인적C2V3편단(558 bp),서렬편집후용Genotyping급Mega 5.03연건공구학정병독기인형,분석HIV-1주적지구화인군분포특정.결과 788례표본획득1728조HIV-1기인서렬,기중전장gag기인서렬599조、전장pol기인서렬564조、env기인C2V3구서렬525조,학정617례기인아형,구성비분별위CRF08 BC(50.2％)、CRF01 AE(25.0％)、미지중조(10.2％)、CRF07_BC(9.2％)、C아형(2.9％)화B(B')아형(2.4％).HIV-1주재운남성구유지역분포특정,가분위이림창화곤명위대표적CRF08_BC위주적지구,이덕굉화서쌍판납위대표적CRF08 _BC여CRF01 _AE구성비상근적지구；미지중조형재운남성소수민족중소점비례(17.0％)현저고우한족(6.7％)；이성성전파감염자화정맥주사흡독감염자적CRF08 BC아형균점총수적50.0％이상,단전자CRF01_AE적구성비점약30.0％,후자미지중조형화CRF07 BC적비례분별체도약15.0％.미지중조병독주정현량충중조모식,분별위B(B')/C중조화이CRF01 AE위모주감입B(B ')화/혹C편단중조,병이전자위주(74.6％).결론 운남성HIV-1주조성복잡,구유현저적지역、민족화전파도경상관적특정,병출현신형중조병독주,응밀절감측.
Objective To understanding the genetic subtype and its population and regional distribution of HIV-1 strains circulating in Yunnan province.Methods 788 plasma specimens collected in 2008-2009 from 15 distracts of Yunnan,were enrolled.Viral RNA were extracted and subjected to RT-PCR.1584 bp full length gag gene,3147 bp full length pol gene and 558 bp env (C2V3) fragment were amplified and directly sequenced.Full length gag and pol genes were connected together as a complete genetic region (location on HXB2:790-5096) for genotyping.Results Of the 788 plasma specimens,a total number of 1728 genomic sequences including 599 gag,564 pol and 525 env (C2V3) were successfully amplified and sequenced,with genotype of 617 samples identified.The subtypes of HIV-1 strains circulated in Yunnan were with the order of constituent ratio CRF08_BC ( 50.2％ ),CRF01_AE (25.0％),unknown recombinant forms ( 10.2％ ),CRF07_BC (9.2％),subtype C (2.9％) and subtype B (B') (2.4％).The distributions of subtypes showed significant regional differences and could be roughly divided into two forms:the CRF08_BC predominant areas represented by Lincang and Kunming,and the areas with CRF08_BC together with CRF01_AE coexistence,represented by Dehong and Xishuangbanna.The unknown recombinant forms accounted for more HIV infection in ethnic minorities (17.0％) than in ethnic Han (6.7％,P＜0.01 ).The distribution of subtypes varied significantly in the two primary routes of transmission for those infected through heterosexual contact.CRF08_BC and CRF0 1_AE were the dominant subtypes,accounting for 52.7％ and 29.1％ respectively.However,in IDUs,CRF08_BC strains accounted for half of the infection,while only 4.5％ of the infections were caused by CRF01_AE,CRF07_BC while the unique recombinant forms were responsible for 15.5％ infections.Of the 63 unknown recombinant forms,most (74.6％) were B (B' ) recombinant with C,while 25％ were mosaic B and/or C fragments on the bases of CRF01_AE genome.Conclusion The subtypes of HIV-1 strains circulated in Yunnan were complicated under the significant differences of regions,ethnics or routes of transmission.
