中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2009年
5期
512-515
,共4页
肾脏%出血性休克复苏%前列腺素El%Caspase-3
腎髒%齣血性休剋複囌%前列腺素El%Caspase-3
신장%출혈성휴극복소%전렬선소El%Caspase-3
Kidney%Hemorrhagic shock and resuscitation(HSR)%Lipo-PGEl%Caspase-3
目的 探讨PGE1预先给药对急性出血性休克复苏后大鼠肾组织活化Caspase-3以及TNF-α mRNA,iNOS mRNA表达的影响.方法 32只雄性SD健康大鼠随机分成4组(n=8),A:正常对照组,不进行任何处理;B:手术对照组,0.5 mL生理盐水尾静脉注射,1 h后切开左侧腹股沟皮肤暴露股动静脉;C:出血性休克复苏组,大鼠硫喷妥纳麻醉后,经股静脉穿刺置管放血,平均动脉血压降至25～35 mmHg,维持1 h,自体温血复苏30 min,维持平均动脉血压80～100 mmHg,制作失血性休克复苏模型;D:出血性休克复苏+lipo-PGEl预先给药组,lipo-PGEl按10 μg/kg溶于0.5 mL生理盐水尾静脉汴射,1 h后制作出血性休克复苏模型.各组分别于复苏后6 h时间点用Northern blotting法检测肾组织TNF-α mRNA和iNOS mRNA的表达;免疫组化检测肾脏组织活化Caspase-3的表达.数据采用单因数方差分析(SNK-q检验),以P<0.05为差异具有统计学意义.结果 出血性休克复苏模型组肾组织出现Caspase-3阳性表达细胞;TNF-α mRNA和iNOS mRNA的表达也明显高于正常对照组[(0.651±0.028)vs.(0.030±0.003),(0.524±0.022)vs.(0.026±0.003),P<0.05]和手术对照组[(0.651±0.028)vs.(0.029±0.002),(0.524±0.022)vs.(0.025±0.003),P<0.05];预先给药组肾组织Caspase-3阳性细胞数量显著减少;TNF-αmRNA和iNOS mRNA的表达明显低于模型组[(0.250±0.019)vs.(0.651±0.028),(0.203±0.020)vs.(0.524±0.022),P<0.05],正常对照组和手术对照组间肾组织Caspase-3的表达以及TNF-α mRNA和iNOS mRNA的表达尢显著差异[(0.029±0.002)vs.(0.030±0.003),(0.029±0.002)vs.(0.030±0.003),P>0.05].结论 lipo-PGEl预先给药可以下调出血性休克复苏后大鼠肾组织Caspase-3的表达水平,其机制可能与在基因转录水平抑制TNF-α和I-NOS的表达有关.
目的 探討PGE1預先給藥對急性齣血性休剋複囌後大鼠腎組織活化Caspase-3以及TNF-α mRNA,iNOS mRNA錶達的影響.方法 32隻雄性SD健康大鼠隨機分成4組(n=8),A:正常對照組,不進行任何處理;B:手術對照組,0.5 mL生理鹽水尾靜脈註射,1 h後切開左側腹股溝皮膚暴露股動靜脈;C:齣血性休剋複囌組,大鼠硫噴妥納痳醉後,經股靜脈穿刺置管放血,平均動脈血壓降至25～35 mmHg,維持1 h,自體溫血複囌30 min,維持平均動脈血壓80～100 mmHg,製作失血性休剋複囌模型;D:齣血性休剋複囌+lipo-PGEl預先給藥組,lipo-PGEl按10 μg/kg溶于0.5 mL生理鹽水尾靜脈汴射,1 h後製作齣血性休剋複囌模型.各組分彆于複囌後6 h時間點用Northern blotting法檢測腎組織TNF-α mRNA和iNOS mRNA的錶達;免疫組化檢測腎髒組織活化Caspase-3的錶達.數據採用單因數方差分析(SNK-q檢驗),以P<0.05為差異具有統計學意義.結果 齣血性休剋複囌模型組腎組織齣現Caspase-3暘性錶達細胞;TNF-α mRNA和iNOS mRNA的錶達也明顯高于正常對照組[(0.651±0.028)vs.(0.030±0.003),(0.524±0.022)vs.(0.026±0.003),P<0.05]和手術對照組[(0.651±0.028)vs.(0.029±0.002),(0.524±0.022)vs.(0.025±0.003),P<0.05];預先給藥組腎組織Caspase-3暘性細胞數量顯著減少;TNF-αmRNA和iNOS mRNA的錶達明顯低于模型組[(0.250±0.019)vs.(0.651±0.028),(0.203±0.020)vs.(0.524±0.022),P<0.05],正常對照組和手術對照組間腎組織Caspase-3的錶達以及TNF-α mRNA和iNOS mRNA的錶達尢顯著差異[(0.029±0.002)vs.(0.030±0.003),(0.029±0.002)vs.(0.030±0.003),P>0.05].結論 lipo-PGEl預先給藥可以下調齣血性休剋複囌後大鼠腎組織Caspase-3的錶達水平,其機製可能與在基因轉錄水平抑製TNF-α和I-NOS的錶達有關.
목적 탐토PGE1예선급약대급성출혈성휴극복소후대서신조직활화Caspase-3이급TNF-α mRNA,iNOS mRNA표체적영향.방법 32지웅성SD건강대서수궤분성4조(n=8),A:정상대조조,불진행임하처리;B:수술대조조,0.5 mL생리염수미정맥주사,1 h후절개좌측복고구피부폭로고동정맥;C:출혈성휴극복소조,대서류분타납마취후,경고정맥천자치관방혈,평균동맥혈압강지25～35 mmHg,유지1 h,자체온혈복소30 min,유지평균동맥혈압80～100 mmHg,제작실혈성휴극복소모형;D:출혈성휴극복소+lipo-PGEl예선급약조,lipo-PGEl안10 μg/kg용우0.5 mL생리염수미정맥변사,1 h후제작출혈성휴극복소모형.각조분별우복소후6 h시간점용Northern blotting법검측신조직TNF-α mRNA화iNOS mRNA적표체;면역조화검측신장조직활화Caspase-3적표체.수거채용단인수방차분석(SNK-q검험),이P<0.05위차이구유통계학의의.결과 출혈성휴극복소모형조신조직출현Caspase-3양성표체세포;TNF-α mRNA화iNOS mRNA적표체야명현고우정상대조조[(0.651±0.028)vs.(0.030±0.003),(0.524±0.022)vs.(0.026±0.003),P<0.05]화수술대조조[(0.651±0.028)vs.(0.029±0.002),(0.524±0.022)vs.(0.025±0.003),P<0.05];예선급약조신조직Caspase-3양성세포수량현저감소;TNF-αmRNA화iNOS mRNA적표체명현저우모형조[(0.250±0.019)vs.(0.651±0.028),(0.203±0.020)vs.(0.524±0.022),P<0.05],정상대조조화수술대조조간신조직Caspase-3적표체이급TNF-α mRNA화iNOS mRNA적표체왕현저차이[(0.029±0.002)vs.(0.030±0.003),(0.029±0.002)vs.(0.030±0.003),P>0.05].결론 lipo-PGEl예선급약가이하조출혈성휴극복소후대서신조직Caspase-3적표체수평,기궤제가능여재기인전록수평억제TNF-α화I-NOS적표체유관.
Objective To observe the effects of pretreatment in rats with prostaglandin E1 on expression of kidney caspase-3, and TNF-α mRNA and iNOS mRNA after hemorrhagic shock and resuscitation(HSR). Method Totally 32 male S-D rots were randomly divided into four groups: group A (normal group, n=8), rats didn't receive any treatment; group B(sham, n=8), rats were pretreated with normal saline and underwent all experi-mentation procedures except bleeding; group C (HSR, n=8): rats were anesthetized with intraperitoneal sodium pentobarbital(30 mg/kg) and then subjected to hemorrhagic shock followed by resuscitation; group D (lipo-PGEl +HSR, n=8): rats were pretreated with lipo-PGEl one hour before HSR. The expressions of TNF-α mRNA and iNOS mRNA was measured by Northern blotting analysis and the expression of caspase-3 at 6 hours was determined by immunohistochemistry after HSR. Data were analyzed by ANOVA (SNK-q test), and P<0.05 was considered as significantly different. Results There were no differences in TNF-α mRNA and iNOS mRNA between normal group and sham group [(0.029±0.002) vs. (0.030±0.003),(0.029±0.002) vs. (0.030±0.003), P> 0.05]. HSR led to overexpression of caspase-3. The expression of kidney TNF-α mRNA and iNOS mRNA also increased in HSR group, compared with normal group [(0.651±0.028) vs. (0.030±0.003), (0.524±0.022) vs. (0.026±0.003), P<0.05] and sham group[(0.651±0.028) vs. (0.029±0.002), (0.524±0.022) vs. (0.025±0.003), P<0.05]. Pre-treatment with lipo-PGEl markedly reduced the expression of Caspase-3, which was consitent with decrease in expressions of TNF-α mRNA and iNOS mRNA 6 hours after HSR[(0.250± 0.019) vs. (0.651±0.028), (0.203±0.020) vs. (0.524±0.022), P<0.050]. Conclusions Lipo-PGEl could reduce the expressions of kidney Caspase-3 after HSR. The mechanisms might be attributed to inhibiting the expression of TNF-α mRNA and iNOS mRNA.
