CAJ | 학술논문

目的本研究探讨了2型糖尿病患者外周血单个核细胞( PBMC)组蛋白乙酰化修饰状态.方法收集12例2型糖尿病患者及12名健康对照者的PBMC,H3/H4乙酰化检测试剂盒提取组蛋白,检测H3/H4乙酰化水平.实时定量PCR检测2型糖尿病PBMC组蛋白修饰基因mRNA表达水平.结果与健康对照组比较,2型糖尿病组PBMC组蛋白H3/H4乙酰化水平升高(H3:0.134±0.035对0.181±0.023,P＜0.05；H4:0.266±0.070对0.324 ±0.062,P＜0.01).2型糖尿病组PBMC的p300、CREBBP、HDAC2、HDAC7、SIRT1基因表达分别是健康对照组的3.11±0.38、2.78±0.45、3.55±0.30、0.77±0.37、0.40±0.25倍.结论 2型糖尿病患者外周血PBMC组蛋白乙酰化修饰呈现异常.
목적 본연구탐토료2형당뇨병환자외주혈단개핵세포( PBMC)조단백을선화수식상태.방법 수집12례2형당뇨병환자급12명건강대조자적PBMC,H3/H4을선화검측시제합제취조단백,검측H3/H4을선화수평.실시정량PCR검측2형당뇨병PBMC조단백수식기인mRNA표체수평.결과 여건강대조조비교,2형당뇨병조PBMC조단백H3/H4을선화수평승고(H3:0.134±0.035대0.181±0.023,P＜0.05；H4:0.266±0.070대0.324 ±0.062,P＜0.01).2형당뇨병조PBMC적p300、CREBBP、HDAC2、HDAC7、SIRT1기인표체분별시건강대조조적3.11±0.38、2.78±0.45、3.55±0.30、0.77±0.37、0.40±0.25배.결론 2형당뇨병환자외주혈PBMC조단백을선화수식정현이상.
Objective To study histone acetylation patterns in peripheral boiood mononuclear cells (PBMC) from type 2 diabetic patients PBMC form 12type 2 diabetic patients and 12 healthy contrcl subiects were collecyed The global histone H3/H4 acetylation was determined by assay kit The differetial expression of the histone acetyltransferases and histone deacetylases were measured by real time PCR.Results increased H3/H4 acetylation was observed in PBMC of type 2 diabetic patients compared with control subjects (H3:0.134±0.035vs0.181±0.032,p<0.05;H4:0.266±0.070VS0.324±0.062,P<0.01).the mRNA levels of p300,CREBBP,HDAC2,HDAC7,and STRTL in patients PBMC compared with healthy control subieects were 3.11±0.38,2.78±0.45,3.55±0.30,0.77±0.37 and 0.40±0.25 folds respectively conclusion histone acetylation appears abnormal in PBMC of type 2 diabetic patients.