中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
6期
467-469
,共3页
周文波%袁方均%邹灿%朱正鹏%马建波%戴宗晴%张有顺
週文波%袁方均%鄒燦%硃正鵬%馬建波%戴宗晴%張有順
주문파%원방균%추찬%주정붕%마건파%대종청%장유순
癌,肝细胞%RNA干扰%Oct4%Wnt/β-catenin通路
癌,肝細胞%RNA榦擾%Oct4%Wnt/β-catenin通路
암,간세포%RNA간우%Oct4%Wnt/β-catenin통로
Carcinoma,hepatocellular%RNA interference%Oct4%Wnt/p-catenin signal pathway
目的 探讨干细胞相关基因Oct4与Wnt/β-catenin在人肝癌组织及肝癌细胞系中的相互作用.方法 PCR法检测Oct4、Wnt/β-catenin及其他相关基因在肝癌组织、癌旁组织及正常肝组织的表达差异.使用SiRNA-Oct4沉默人肝癌HepG2细胞Oct4的表达,实时荧光定量PCR法检测Wnt/β-catenin基因表达.检测RNAi后细胞迁移以及克隆形成能力.结果 肝癌患者的上述组织中,Oct4和β-catenin在癌内表达最高,癌旁次之,正常肝最低.使用SiRNA-Oct4沉默人肝癌HepG2细胞Oct4后,Oct4表达明显下调,β-catenin、Wnt10b表现为与Oct4表达呈正相关,TCF3表达与Oct4呈负相关.RNAi后HepG2细胞的迁移能力、克隆形成能力均下降.结论 Oct4在肝癌组织内高表达而在正常肝细胞中的表达极低.RNAi后HepG2细胞的迁移能力、克隆形成能力均下降可能与Wnt/β-catenin通路功能减弱有关.
目的 探討榦細胞相關基因Oct4與Wnt/β-catenin在人肝癌組織及肝癌細胞繫中的相互作用.方法 PCR法檢測Oct4、Wnt/β-catenin及其他相關基因在肝癌組織、癌徬組織及正常肝組織的錶達差異.使用SiRNA-Oct4沉默人肝癌HepG2細胞Oct4的錶達,實時熒光定量PCR法檢測Wnt/β-catenin基因錶達.檢測RNAi後細胞遷移以及剋隆形成能力.結果 肝癌患者的上述組織中,Oct4和β-catenin在癌內錶達最高,癌徬次之,正常肝最低.使用SiRNA-Oct4沉默人肝癌HepG2細胞Oct4後,Oct4錶達明顯下調,β-catenin、Wnt10b錶現為與Oct4錶達呈正相關,TCF3錶達與Oct4呈負相關.RNAi後HepG2細胞的遷移能力、剋隆形成能力均下降.結論 Oct4在肝癌組織內高錶達而在正常肝細胞中的錶達極低.RNAi後HepG2細胞的遷移能力、剋隆形成能力均下降可能與Wnt/β-catenin通路功能減弱有關.
목적 탐토간세포상관기인Oct4여Wnt/β-catenin재인간암조직급간암세포계중적상호작용.방법 PCR법검측Oct4、Wnt/β-catenin급기타상관기인재간암조직、암방조직급정상간조직적표체차이.사용SiRNA-Oct4침묵인간암HepG2세포Oct4적표체,실시형광정량PCR법검측Wnt/β-catenin기인표체.검측RNAi후세포천이이급극륭형성능력.결과 간암환자적상술조직중,Oct4화β-catenin재암내표체최고,암방차지,정상간최저.사용SiRNA-Oct4침묵인간암HepG2세포Oct4후,Oct4표체명현하조,β-catenin、Wnt10b표현위여Oct4표체정정상관,TCF3표체여Oct4정부상관.RNAi후HepG2세포적천이능력、극륭형성능력균하강.결론 Oct4재간암조직내고표체이재정상간세포중적표체겁저.RNAi후HepG2세포적천이능력、극륭형성능력균하강가능여Wnt/β-catenin통로공능감약유관.
Objective To investigate the expression of Oct4 in liver cancer, and the interrelation of the Oct4 and Wnt/β-catenin genes in hepatocellular carcinoma( HCC) cell line HepG2. Methods RTPCR technique was used to detect the expression of Oct4 and β-Catenin in HCC specimens; RNAi was used to knock-down the expression of Oct4 in HepG2, and the change of Wnt/β-catenin related genes were detected by Real time-PCR. Results In HCC specimens, the expression of Oct4 and β-Catenin in tumor and cirrhotic liver tissues were stronger than normal liver tissues. In SiRNA Oct4 HepG2 cells, the expression of Oct4 was downregulated, and β-catenin as well as Wnt10b were in a positive correlation with Oct4, TCF3 was in negative correlation with Oct4. Clone formation and move ability of the HepG2 were downregulated. Conclusions The expression of Oct4 was higher in tumor tissues than in normal liver tissues. Silencing Oct4 by SiRNA-0ct4 in HepG2 resulted in decreased ability of clone formation and cell movement.
