CAJ | 학술논문

目的了解分离自烧伤患者创面的铜绿假单胞菌(PA)耐药性与菌株中质粒、转座子、整合子3种可移动遗传元件的关系.方法 32株PA分离自宁波市第二医院烧伤患者创面分泌物标本.采用GNS-448药敏卡及K-B纸片扩散法测定PA的药物敏感性.采用PCR扩增及序列分析法,检测PA中质粒、转座子、整合子遗传标记基因traA、traF、tnpA、tnpU、merA和jnt I 1.结果 32株PA对庆大霉素、阿米卡星、头孢哌酮/舒巴坦、环丙沙星的耐药率分别为43.7%、32.0%、46.8%、49.9%,对哌拉西林、头孢噻肟、头孢他啶、头孢吡肟、氨曲南、哌拉西林/他唑巴坦、左氧氟沙星、亚胺培南、美罗培南的耐药率均在56.0%以上.32株PA中17株携带了转座子和(或)整合子遗传标记,其中tnpA与merA同时阳性1株,merA与int I 1同时阳性8株;单独tnpA阳性1株,单独merA阳性2株,单独int I 1阳性5株.结论分离自宁波市第二医院烧伤患者创面的PA耐药情况严重,可能与其转座子和(或)整合子携带率较高有关.
목적 료해분리자소상환자창면적동록가단포균(PA)내약성여균주중질립、전좌자、정합자3충가이동유전원건적관계.방법 32주PA분리자저파시제이의원소상환자창면분비물표본.채용GNS-448약민잡급K-B지편확산법측정PA적약물민감성.채용PCR확증급서렬분석법,검측PA중질립、전좌자、정합자유전표기기인traA、traF、tnpA、tnpU、merA화jnt I 1.결과 32주PA대경대매소、아미잡성、두포고동/서파탄、배병사성적내약솔분별위43.7%、32.0%、46.8%、49.9%,대고랍서림、두포새우、두포타정、두포필우、안곡남、고랍서림/타서파탄、좌양불사성、아알배남、미라배남적내약솔균재56.0%이상.32주PA중17주휴대료전좌자화(혹)정합자유전표기,기중tnpA여merA동시양성1주,merA여int I 1동시양성8주;단독tnpA양성1주,단독merA양성2주,단독int I 1양성5주.결론 분리자저파시제이의원소상환자창면적PA내약정황엄중,가능여기전좌자화(혹)정합자휴대솔교고유관.
Objective To investigate the relationship between the drug resistance of Pseudomonas aeruginosa (PA) isolated from burn patients wounds and its mobile genetic elements, including plasmid, transposon, and integron. Methods Thirty-two strains of PA were isolated from wounds exudate of hospi-talized burn patients in Ningbo No. 2 Hospital. PA drug sensitivity was determined using GNS-448 drug sen-sitivity card and K-B tests. The genetic markers of plasmid, transposon and integron including traA, traF, tnpA, tnpU, merA, int I 1 were amplified by PCR and verified by gene sequencing. Results Drug resist-ant rate of 32 PA strains to gentamicin, amikaein, eefoperazone/sulbaetam, ciprofloxaein was 43. 7%, 32. 0%, 46. 8%, 49.9%, respectively. PA drug resistant rates to piperacillin, cefotaxime, ecflazidime, cefepime, aztreonam, piperaeillin/tazobactam, levofloxacin, imipenem and meropenem were all above 56. 0%. Seventeen out of 32 PA strains were found to carry transposon and (or) integron genetic markers, One strain was positive for both tnpA and mcrA, 8 strains were positive for both merA and int I 1, 1 strain was only positive for tnpA, 2 strains were only positive for merA, and 5 strains were positive for int I 1 only. Conclusions PA isolated from burn wounds of hospitalized patients in Ningbo No. 2 Hospital is seriously drug resistant, which may relate with its high positive rate of mobile genetic elements of transposon and (or) integron.