中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
4期
446-450
,共5页
俞秋兴%唐军%颜茹红%朱雪明%顾爱萍
俞鞦興%唐軍%顏茹紅%硃雪明%顧愛萍
유추흥%당군%안여홍%주설명%고애평
细胞因子类%T淋巴细胞,辅助诱导%T淋巴细胞,调节性
細胞因子類%T淋巴細胞,輔助誘導%T淋巴細胞,調節性
세포인자류%T림파세포,보조유도%T림파세포,조절성
Cytokines%T-lymphocytes,helper-inducer%T-lymphocytes,regulatory
目的 探讨多种细胞因子包括转化生长因子β(TGF-β)、白细胞介素6(IL-6)、白细胞介素2(IL-2)对CD+4T淋巴细胞的分化调节作用.方法 将人外周血T淋巴细胞、鼠脾脏T淋巴细胞在不同的刺激条件下进行体外培养,采用流式细胞仪分析活化T淋巴细胞中CD+4IL-17+T辅助17细胞(Th17细胞)、C+4IL-17+T淋巴细胞、CD+4CD+25FOXP+3T调节性细胞(Treg细胞)的表达情况.结果 鼠脾脏T淋巴细胞培养液中有TGF-β存在时可促进Treg细胞、rh17细胞和CD+8;IL-17+T淋巴细胞分化,表达水平分别为(7.8±2.2)%、(12.6±3.1)%、(10.1±2.6)%.无细胞因子培养的同类细胞为实验对照组,表达水平分别为(4.8±0.6)%、(1.7±0.5)%、(1.0±0.4)%,差异均有统计学意义(q=4.09、8.80、9.61,P<0.05或P<0.01).TGF-β与IL-6细胞因子共同存在时可促进Th17和CD+8IL-17+T淋巴细胞分化,表达水平分别为(17.8±5.3)%、(15.0±4.2)%,而分化受抑制的Treg细胞的表达水平为(4.I±1.2)%,与TGF-β组同类细胞表达水平比较差异均有统计学意义(q=5.03、5.17、5.04,P均<0.01).在TGF-β刺激的T淋巴细胞中加入IL-2细胞因子可使Th17细胞、CD+8;IL-17+T淋巴细胞表达减少,同时伴有Treg细胞表达量的增加;在加入抗IL-2后结果相反,即Th17细胞、CD+8IL-17+T淋巴细胞表达水平增加,Treg细胞表达水平减少.人外周血T淋巴细胞上Th17、CD+8IL-17+、Treg细胞表达水平变化趋势与鼠脾脏淋巴细胞相似.结论 不同细胞因子对于调控Th17细胞和Treg细胞之间的平衡起着十分重要的作用.
目的 探討多種細胞因子包括轉化生長因子β(TGF-β)、白細胞介素6(IL-6)、白細胞介素2(IL-2)對CD+4T淋巴細胞的分化調節作用.方法 將人外週血T淋巴細胞、鼠脾髒T淋巴細胞在不同的刺激條件下進行體外培養,採用流式細胞儀分析活化T淋巴細胞中CD+4IL-17+T輔助17細胞(Th17細胞)、C+4IL-17+T淋巴細胞、CD+4CD+25FOXP+3T調節性細胞(Treg細胞)的錶達情況.結果 鼠脾髒T淋巴細胞培養液中有TGF-β存在時可促進Treg細胞、rh17細胞和CD+8;IL-17+T淋巴細胞分化,錶達水平分彆為(7.8±2.2)%、(12.6±3.1)%、(10.1±2.6)%.無細胞因子培養的同類細胞為實驗對照組,錶達水平分彆為(4.8±0.6)%、(1.7±0.5)%、(1.0±0.4)%,差異均有統計學意義(q=4.09、8.80、9.61,P<0.05或P<0.01).TGF-β與IL-6細胞因子共同存在時可促進Th17和CD+8IL-17+T淋巴細胞分化,錶達水平分彆為(17.8±5.3)%、(15.0±4.2)%,而分化受抑製的Treg細胞的錶達水平為(4.I±1.2)%,與TGF-β組同類細胞錶達水平比較差異均有統計學意義(q=5.03、5.17、5.04,P均<0.01).在TGF-β刺激的T淋巴細胞中加入IL-2細胞因子可使Th17細胞、CD+8;IL-17+T淋巴細胞錶達減少,同時伴有Treg細胞錶達量的增加;在加入抗IL-2後結果相反,即Th17細胞、CD+8IL-17+T淋巴細胞錶達水平增加,Treg細胞錶達水平減少.人外週血T淋巴細胞上Th17、CD+8IL-17+、Treg細胞錶達水平變化趨勢與鼠脾髒淋巴細胞相似.結論 不同細胞因子對于調控Th17細胞和Treg細胞之間的平衡起著十分重要的作用.
목적 탐토다충세포인자포괄전화생장인자β(TGF-β)、백세포개소6(IL-6)、백세포개소2(IL-2)대CD+4T림파세포적분화조절작용.방법 장인외주혈T림파세포、서비장T림파세포재불동적자격조건하진행체외배양,채용류식세포의분석활화T림파세포중CD+4IL-17+T보조17세포(Th17세포)、C+4IL-17+T림파세포、CD+4CD+25FOXP+3T조절성세포(Treg세포)적표체정황.결과 서비장T림파세포배양액중유TGF-β존재시가촉진Treg세포、rh17세포화CD+8;IL-17+T림파세포분화,표체수평분별위(7.8±2.2)%、(12.6±3.1)%、(10.1±2.6)%.무세포인자배양적동류세포위실험대조조,표체수평분별위(4.8±0.6)%、(1.7±0.5)%、(1.0±0.4)%,차이균유통계학의의(q=4.09、8.80、9.61,P<0.05혹P<0.01).TGF-β여IL-6세포인자공동존재시가촉진Th17화CD+8IL-17+T림파세포분화,표체수평분별위(17.8±5.3)%、(15.0±4.2)%,이분화수억제적Treg세포적표체수평위(4.I±1.2)%,여TGF-β조동류세포표체수평비교차이균유통계학의의(q=5.03、5.17、5.04,P균<0.01).재TGF-β자격적T림파세포중가입IL-2세포인자가사Th17세포、CD+8;IL-17+T림파세포표체감소,동시반유Treg세포표체량적증가;재가입항IL-2후결과상반,즉Th17세포、CD+8IL-17+T림파세포표체수평증가,Treg세포표체수평감소.인외주혈T림파세포상Th17、CD+8IL-17+、Treg세포표체수평변화추세여서비장림파세포상사.결론 불동세포인자대우조공Th17세포화Treg세포지간적평형기착십분중요적작용.
Objective To investigate the roles of a variety of cytokines including transforming growth factor beta (TGF-β),interleukin-6 (IL-6) and interleukin-2 (IL-2) in the differentiation of CD+4 Tlymphocyte cells.Methods T lymphocyte cells either in human peripheral blood or routine spleen were cultured in vitro under different stimulation conditions.Flow cytometry was used to analyze the percentages of CD+4IL-17+ T-helper 17(Th17) cells,CD+8 IL-17+ T cells,CD+4 CD+25 FOXP+3 T regulatory (Treg) cells among activated T cells.Results Differentiation of Treg cells,Th17 cells and CD+8 IL-17+ T lymphocyte cells was enhanced when murine splenic T cells were cultured with TGF-β.The levels of expression were (7.8±2.2)%,(12.6±3.1)%,(10.1±2.6)% ,respectively.Experimental control group was severally same type of T cells without cytokine treatment.The levels of expression were (4.8±0.6) %,(1.7±0.5) %,(1.0±0.4) %,respectively.There were statistically significant differences among them (q=4.09,8.80,9.61.P<0.05 or P<0.01).Under combination treatment with IL-6 and TGF-β,(17.8±5.3) % Th17 cells and (15.0±4.2)% CDCD+8 IL-17CD+ T cells were induced,whereas the levels of Treg cells whose differentiation were restrained were (4.1±1.2) %.The differences were statistically significant compared with the level of same type of T cells in TGF-β group (q=5.03,5.17,5.04,P<0.01).Moreover,combination treatment with IL-2 and TGF-β decreased percentages of Th17 and CDCD+8 IL-17CD+ T cells and increased percentages of Treg cells in T cell population.There was an opposite effect when anti-IL-2 was apphed.The percentages of Th17 and CD+8 IL-17+ T cells were increased and the percentages of Treg cells were reduced The regulation trend of T lymphocyte cells in human peripheral blood was similar with those in routine spleen.Conclusion Various cytokines are of great importance in the regulation of the balance between Th17 and Treg cell.
