中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2009年
1期
29-32
,共4页
邵泽叶%唐萌%陈宝安%夏国华%张林%朱怀刚
邵澤葉%唐萌%陳寶安%夏國華%張林%硃懷剛
소택협%당맹%진보안%하국화%장림%주부강
纳米粒%砷剂%骨髓增生异常综合征%MUTZ-1细胞%细胞凋亡
納米粒%砷劑%骨髓增生異常綜閤徵%MUTZ-1細胞%細胞凋亡
납미립%신제%골수증생이상종합정%MUTZ-1세포%세포조망
Nanoparticles%Arsenicals%Myelodysplastic syndrome%MUTZ-1 cell line%Cell apoptosis
目的 探讨纳米三硫化三砷(As2S3)与传统剂型As2S3对人骨髓增生异常综合征(MDS)细胞株MUTZ-1的生长抑制作用及其可能的机制.方法 用MTT法比较两种剂型的As2S3对MUTZ-1细胞的生长抑制作用;透射电子显微镜技术分析细胞形态和超微结构的变化;流式细胞术分析细胞凋亡和周期的改变;发光比色法分析caspase-3活性.结果 2、4、8、16 μmol/L两种剂型As2S3分别处理MUTZ-1细胞48 h后,纳米As2S3对细胞的抑制率分别为48.9%、75.9%、89.4%和96.5%,而传统剂型As2S3组分别为14.5%、25.4%、34.7%和51.5%,两组相比差异有统计学意义(P值均<0.01);纳米As2S3组细胞凋亡率分别为(12.9±1.9)%、(19.2±2.2)%、(30.1±2.5)%和(45.9±2.3)%,而传统剂型As2S3组分别为(5.3±1.8)%、(11.1±2.6)%、(19.3±2.3)%和(25.5±2.5)%,两组相比差异也有统计学意义(P值均<0.01);纳米As2S3组G2/M期细胞比例、caspase-3活性明显高于传统剂型As2S3组(P<0.01),且以上各指标均呈浓度-时间依赖性.结论 两种剂型As2S3对MUTZ-1细胞均有生长抑制和诱导凋亡作用,其机制可能与G2/M期细胞阻滞以及caspase-3活化有关;与传统剂型的As2S3相比,纳米As2S3有更强的抗肿瘤作用.
目的 探討納米三硫化三砷(As2S3)與傳統劑型As2S3對人骨髓增生異常綜閤徵(MDS)細胞株MUTZ-1的生長抑製作用及其可能的機製.方法 用MTT法比較兩種劑型的As2S3對MUTZ-1細胞的生長抑製作用;透射電子顯微鏡技術分析細胞形態和超微結構的變化;流式細胞術分析細胞凋亡和週期的改變;髮光比色法分析caspase-3活性.結果 2、4、8、16 μmol/L兩種劑型As2S3分彆處理MUTZ-1細胞48 h後,納米As2S3對細胞的抑製率分彆為48.9%、75.9%、89.4%和96.5%,而傳統劑型As2S3組分彆為14.5%、25.4%、34.7%和51.5%,兩組相比差異有統計學意義(P值均<0.01);納米As2S3組細胞凋亡率分彆為(12.9±1.9)%、(19.2±2.2)%、(30.1±2.5)%和(45.9±2.3)%,而傳統劑型As2S3組分彆為(5.3±1.8)%、(11.1±2.6)%、(19.3±2.3)%和(25.5±2.5)%,兩組相比差異也有統計學意義(P值均<0.01);納米As2S3組G2/M期細胞比例、caspase-3活性明顯高于傳統劑型As2S3組(P<0.01),且以上各指標均呈濃度-時間依賴性.結論 兩種劑型As2S3對MUTZ-1細胞均有生長抑製和誘導凋亡作用,其機製可能與G2/M期細胞阻滯以及caspase-3活化有關;與傳統劑型的As2S3相比,納米As2S3有更彊的抗腫瘤作用.
목적 탐토납미삼류화삼신(As2S3)여전통제형As2S3대인골수증생이상종합정(MDS)세포주MUTZ-1적생장억제작용급기가능적궤제.방법 용MTT법비교량충제형적As2S3대MUTZ-1세포적생장억제작용;투사전자현미경기술분석세포형태화초미결구적변화;류식세포술분석세포조망화주기적개변;발광비색법분석caspase-3활성.결과 2、4、8、16 μmol/L량충제형As2S3분별처리MUTZ-1세포48 h후,납미As2S3대세포적억제솔분별위48.9%、75.9%、89.4%화96.5%,이전통제형As2S3조분별위14.5%、25.4%、34.7%화51.5%,량조상비차이유통계학의의(P치균<0.01);납미As2S3조세포조망솔분별위(12.9±1.9)%、(19.2±2.2)%、(30.1±2.5)%화(45.9±2.3)%,이전통제형As2S3조분별위(5.3±1.8)%、(11.1±2.6)%、(19.3±2.3)%화(25.5±2.5)%,량조상비차이야유통계학의의(P치균<0.01);납미As2S3조G2/M기세포비례、caspase-3활성명현고우전통제형As2S3조(P<0.01),차이상각지표균정농도-시간의뢰성.결론 량충제형As2S3대MUTZ-1세포균유생장억제화유도조망작용,기궤제가능여G2/M기세포조체이급caspase-3활화유관;여전통제형적As2S3상비,납미As2S3유경강적항종류작용.
Objective To in vitro study the inhibition effect and possible mechanism of As2S3 nanop-articles(As2S3 nano) on human MDS cell line MUTZ-1 and to compare with that of traditional As2S3. Meth-od MUTZ-1 cells were treated with As2S3 nano and tranditional regular-sized particles (TRSP) at different concentrations. The cell growth inhibition rate was determined by MTT assay, cell apoptosis by morphplogy and flow cytometry (FCM), cell cycle by FCM and the activity of caspase-3 by chemiluminescence assay. Results Treatment of As2S3 nano and TRSP at concentrations of 2, 4, 8 and 16 μmol/L for 48 h could lead to a significant dose-dependent decrease of MUTZ-1 cells and induce apoptosis. The percentages of inhibition were 48.9%, 75.9%, 89.4% and 96.5% in As2S3 nano vs 14.5%, 25.4%, 34.7% and 51.5% in TRSP and apoptosis rates were (12.9±1.9)% ,(19.2±2.2)% ,(30.1±2.5)% and (45.9±2.3)% in As2S3 nanovs (5.3±1.8%)%,(11.1±2.6)%, (19.3±2.3)% and (25.5±2.5)% in TRSP respectively.There was statistically significant difference in these two groups(P <0.01). The proportion of cell in G2/M phase and the activity of caspase-3 of MUTZ-1 cells treated with As2S3 nano were significantly higher than those treated with control group and As2S3 TRSP groups (P <0.01). Conclusions As2S3 nanoparticles and TRSP can inhibit the proliferation of MUTZ-1 cells and induce apoptosis, which maybe through activating caspase-3 pathways and increasing the proportion of G2/M phase. As2S3 nanoparticles can produce a much better antitumor effect than As2S3 TRSP do.
