农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2009年
10期
2185-2188
,共4页
张洪生%梁军锋%张克强%陈秀为%赵润%张金凤
張洪生%樑軍鋒%張剋彊%陳秀為%趙潤%張金鳳
장홍생%량군봉%장극강%진수위%조윤%장금봉
侧耳属%固态发酵%小麦秸秆%细胞壁化学组分%粗蛋白%瘤胃消化率
側耳屬%固態髮酵%小麥秸稈%細胞壁化學組分%粗蛋白%瘤胃消化率
측이속%고태발효%소맥갈간%세포벽화학조분%조단백%류위소화솔
Pleurotus%solid state fermentation%wheat straw%cell wall constituent%crude proteins%rumen digestibility
小麦秸秆木质素含量高,蛋白质等营养物质含量低,作为反刍家畜的粗饲料营养价值低,饲料化利用受到限制.通过室内小麦秸秆固态发酵试验,研究了两株侧耳属真菌Tf1(Pleurotus sajor-caju)和JG1(Pleurotus Cornucopiae Roll)对小麦秸秆细胞壁化学组分的降解、瘤胃消化率和粗蛋白含量的影响.结果表明,经菌株JG1、Tf1和两菌复合发酵21 d的小麦秸秆,木质素降解率分别为28.20%、30.78%和38.41%,纤维素降解率分别为19.26%、19.28%和26.65%;48 h干物质瘤胃消化率分别比未发酵秸秆提高了38.62%、44.81%和55.89%,中性洗涤纤维(NDF)消化率分别提高了38.91%、49.00%和63.08%;粗蛋白含量分别比未发酵秸秆提高了58.60%、69.53%和72.22%.表明菌株JG1和Tf1在选择性降解木质素,改善瘤胃消化率,提高粗蛋白含量方面的优势,而且两菌株复合发酵具有协同作用,与单菌发酵相比.木质素降解率、瘤胃消化率和粗蛋白含量都明显提高.
小麥秸稈木質素含量高,蛋白質等營養物質含量低,作為反芻傢畜的粗飼料營養價值低,飼料化利用受到限製.通過室內小麥秸稈固態髮酵試驗,研究瞭兩株側耳屬真菌Tf1(Pleurotus sajor-caju)和JG1(Pleurotus Cornucopiae Roll)對小麥秸稈細胞壁化學組分的降解、瘤胃消化率和粗蛋白含量的影響.結果錶明,經菌株JG1、Tf1和兩菌複閤髮酵21 d的小麥秸稈,木質素降解率分彆為28.20%、30.78%和38.41%,纖維素降解率分彆為19.26%、19.28%和26.65%;48 h榦物質瘤胃消化率分彆比未髮酵秸稈提高瞭38.62%、44.81%和55.89%,中性洗滌纖維(NDF)消化率分彆提高瞭38.91%、49.00%和63.08%;粗蛋白含量分彆比未髮酵秸稈提高瞭58.60%、69.53%和72.22%.錶明菌株JG1和Tf1在選擇性降解木質素,改善瘤胃消化率,提高粗蛋白含量方麵的優勢,而且兩菌株複閤髮酵具有協同作用,與單菌髮酵相比.木質素降解率、瘤胃消化率和粗蛋白含量都明顯提高.
소맥갈간목질소함량고,단백질등영양물질함량저,작위반추가축적조사료영양개치저,사료화이용수도한제.통과실내소맥갈간고태발효시험,연구료량주측이속진균Tf1(Pleurotus sajor-caju)화JG1(Pleurotus Cornucopiae Roll)대소맥갈간세포벽화학조분적강해、류위소화솔화조단백함량적영향.결과표명,경균주JG1、Tf1화량균복합발효21 d적소맥갈간,목질소강해솔분별위28.20%、30.78%화38.41%,섬유소강해솔분별위19.26%、19.28%화26.65%;48 h간물질류위소화솔분별비미발효갈간제고료38.62%、44.81%화55.89%,중성세조섬유(NDF)소화솔분별제고료38.91%、49.00%화63.08%;조단백함량분별비미발효갈간제고료58.60%、69.53%화72.22%.표명균주JG1화Tf1재선택성강해목질소,개선류위소화솔,제고조단백함량방면적우세,이차량균주복합발효구유협동작용,여단균발효상비.목질소강해솔、류위소화솔화조단백함량도명현제고.
Wheat straw is characterized by its contents of high lignin and low crude proteins, suggesting that the straw limit its utilization as animal feedstuff. The present study was conducted to investigate effects of two fungal strains, JG1( Pleurotus Cornucopias Roll) and Tf1(Pleu-rotus sajor-caju), on cell wall constituent, rumen digestibility and crude protein contents of the wheat straw after the substrate had been treated by solid state fermentation. The fermentation of the straw was carried out for 21 days in the presence of either JG1, Tf1 solely, or com-bination of Tf1+JG1. It has been discovered that the degradation rates of lignin increased by 28.20%, 30.78% and 38.41%, respectively, while the degradation rates of cellulose enhanced by 19.26%, 19.28% and 26.65%, respectively, for the 3 treatments, as compared with the controls. In addition, the 48 h rumen dry matter digestibilities were found to be 38.62%, 44.81% and 55.89% greater than the controls; the rumen neutral detergent fiber digestibilities were discovered to be 38.91%, 49.00% and 63.08% higher than the controls, while the crude pro-tein contents were 58.60%, 69.53% and 72.22% more than the controls, respectively, for the three corresponding treatments. The results mentioned above showed that both species of Pleurotus, JG1, and Tf1 are capable of degradating selectively lignin, improving rumen di-gestibility and increasing crude protein contents in the wheat straw studied. Due to the fact that treatment 3 with a mixture of two species of Pleurotus was obviously characterized by its stronger degradation rates of lignin, higher rumen digestibility and contents of crude proteins than other two treatments, it was likely concluded that there was a synergistic effect on lignin degradation, rumen digestibility and crude protein for the treatment with a combination of JG1+Tf1.