生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
32-34
,共3页
张宏%李佳%库尔班·吐松%苏力坦·阿巴白克力
張宏%李佳%庫爾班·吐鬆%囌力坦·阿巴白剋力
장굉%리가%고이반·토송%소력탄·아파백극력
短毛柽柳%黄酮化合物%超声波法%稳定性%正交试验
短毛檉柳%黃酮化閤物%超聲波法%穩定性%正交試驗
단모정류%황동화합물%초성파법%은정성%정교시험
Tamarix karelini Bge.%flavonoids%ultrasonic wave treatment%stability%orthogonal experiment
目的:比较短毛柽柳中不同部位总黄酮的含量差异,研究黄酮化合物保存的条件,方法:采用超声波乙醇浸提法,在正交优先的最佳条件(料液比为1:15,超声处理时间为40min,乙醇浓度为45%,超声温度50℃,提取3次)下提取不同部位总黄酮,并测定不同条件下其吸光度的变化.结果:柽柳花、叶、茎中总黄酮得率分别为24.585mg/g、25.623mg/g、41.726mg/g,柽柳中提取的黄酮溶液在光照、高温、高浓度蔗糖溶液和Na~+存在条件下不稳定;pH 4~7条件下较稳定;氧化剂对其稳定性的影响不大.结论:柽柳茎中总黄酮含量明显高于其他两个部位.柽柳黄酮保存时应避免接触碳水化合物和盐离子,在避光、常温、中性条件下保存.
目的:比較短毛檉柳中不同部位總黃酮的含量差異,研究黃酮化閤物保存的條件,方法:採用超聲波乙醇浸提法,在正交優先的最佳條件(料液比為1:15,超聲處理時間為40min,乙醇濃度為45%,超聲溫度50℃,提取3次)下提取不同部位總黃酮,併測定不同條件下其吸光度的變化.結果:檉柳花、葉、莖中總黃酮得率分彆為24.585mg/g、25.623mg/g、41.726mg/g,檉柳中提取的黃酮溶液在光照、高溫、高濃度蔗糖溶液和Na~+存在條件下不穩定;pH 4~7條件下較穩定;氧化劑對其穩定性的影響不大.結論:檉柳莖中總黃酮含量明顯高于其他兩箇部位.檉柳黃酮保存時應避免接觸碳水化閤物和鹽離子,在避光、常溫、中性條件下保存.
목적:비교단모정류중불동부위총황동적함량차이,연구황동화합물보존적조건,방법:채용초성파을순침제법,재정교우선적최가조건(료액비위1:15,초성처리시간위40min,을순농도위45%,초성온도50℃,제취3차)하제취불동부위총황동,병측정불동조건하기흡광도적변화.결과:정류화、협、경중총황동득솔분별위24.585mg/g、25.623mg/g、41.726mg/g,정류중제취적황동용액재광조、고온、고농도자당용액화Na~+존재조건하불은정;pH 4~7조건하교은정;양화제대기은정성적영향불대.결론:정류경중총황동함량명현고우기타량개부위.정류황동보존시응피면접촉탄수화합물화염리자,재피광、상온、중성조건하보존.
Objective: To compare the content of total flavones in different parts of short-haired Tamarix karelini Bge, and study the preservative conditions of flavonoid compounds. Method: To apply ethanol extraction with Ultrasonic to extract total flavonoids of different parts, under the best priority orthogonal conditions (material to liquid's ratio 1: 15, ultrasonic time 40 mins, ethanol concentration 45%, ultrasound temperature 50℃, extracted 3 times). and measured its absorbance under different conditions. Result: The yield of flower, browse and caudex isoflavones in the extracts respectively were 24.585mg/g, 25.623 mg/g, 41.726mg/g. The flavanone solution will be decompose action by high temperature and natural light irradiate directly; it is unstable around metalion of Na~+ and higher sucrose condition; the pH value of flavanone solution are stable well between pH 4 and 7; and that is stable in oxidant of H_2O_2 . Conclusion: The flavonoids content in caudex is apparently higher than other two regions, This method is a purely physical process, and has not any pollution; it is an ideal way to extract the flavanone of Tamarix karelini Bge.