CAJ | 학술논문

目的:分析人肺腺癌干细胞(lung adenocarcinoma stem cell, LASC)对顺铂(cisplatin,DDP)和卡铂(carboplatin,CBP)的药物敏感度.方法:人肺腺癌细胞SPC-A1、AG及CPA-Y2经DDP和CBP作用后,应用CCK-8(cell counting kit-8)比色法检测细胞存活率,应用免疫荧光检测法测定药物生存细胞(drug surviving cell,DSC)的表型特征.通过磁性细胞分选技术分离肺腺癌干细胞,应用绿色荧光蛋白(green fluorescent protein,GFP)示踪技术检测DSC中的肿瘤干细胞,分析DSC对DDP和CBP的药物敏感度.结果:LASC具有细支气管肺泡干细胞(bronchioalveolar stem cell, BASC)表型(OCT4~+CCSP~+SP-C~+).将此类癌干细胞(CD221~+)与肺腺癌分化细胞(CD221~-)混合后检测DSC发现,后者具有OCT4~+BASC表型.DSC对铂类药物具有显著抗性.结论:肺腺癌干细胞对DDP和CBP具有显著耐药性,可能是化疗后肿瘤复发的根源.
목적:분석인폐선암간세포(lung adenocarcinoma stem cell, LASC)대순박(cisplatin,DDP)화잡박(carboplatin,CBP)적약물민감도.방법:인폐선암세포SPC-A1、AG급CPA-Y2경DDP화CBP작용후,응용CCK-8(cell counting kit-8)비색법검측세포존활솔,응용면역형광검측법측정약물생존세포(drug surviving cell,DSC)적표형특정.통과자성세포분선기술분리폐선암간세포,응용록색형광단백(green fluorescent protein,GFP)시종기술검측DSC중적종류간세포,분석DSC대DDP화CBP적약물민감도.결과:LASC구유세지기관폐포간세포(bronchioalveolar stem cell, BASC)표형(OCT4~+CCSP~+SP-C~+).장차유암간세포(CD221~+)여폐선암분화세포(CD221~-)혼합후검측DSC발현,후자구유OCT4~+BASC표형.DSC대박류약물구유현저항성.결론:폐선암간세포대DDP화CBP구유현저내약성,가능시화료후종류복발적근원.
Objective:To analyze the drug sensitivity of human lung adenocarcinoma stem cells (LASC) to cisplatin (DDP) and carboplatin (CBP). Methods:Human lung adenocarcinomaic cells SPC-A1,AG,and CPA-Y2 were treated with DDP and CBP. The cell viability of cells was detected by CCK-8 assay. The phenotypic characteristics of drug surviving cells(DSCs)were determined by immunofluorescence staining. The LASC population was then separated by magnetic-activated cell sorting method. The LASC in DSCs was traced by using green fluorescence protein (GFP). The drug sensitivity of DSCs to DDP and CBP was analyzed.Results:The LASC exhibited the phenotypes of bronchioalveolar stem cells (BASC, OCT4~+CCSP~+SP-C~+). After mixture of CD221~+LASC with CD221~-lung adenocarcinoma differentiated cells, the DSC population showed OCT4~+BASC phenotypes. These DSCs were significantly resistant to DDP and CBP.Conclusion:LASC has a high resistance to DDP and CBP. This may be the reason for tumor recurrence after chemotherapy.