CAJ | 학술논문

目的研究不同强度的磁性附着体模拟静磁场对成骨细胞转化生长因子β1(TGF-β1)基因表达的影响.方法利用细胞静磁场加载装置,对体外培养的SD大鼠成骨细胞分别进行12.5、125和250 mT的静磁场加载,连续加载0.5、1、3、5和7d,采用反转录聚合酶链反应(RT-PCR)技术检测成骨细胞的TGF-β1 mRNA表达的.结果 12.5 mT磁场加载组在加载0.5、1和3d,细胞的TGF-βl mRNA的表达比对照组相应时段明显增强(P＜0.05).125和250 mT磁场组加载静磁场0.5、1、3、5和7d后,细胞的TGF-β1 mRNA的表达升高更加明显,与对照组和12.5mT磁场加载组细胞相比差异有统计学意义(P＜0.05).静磁场对细胞的TGF-β1 mRNA表达的影响有一定的剂量-效应关系.结论在本试验条件下,磁性附着体模拟静磁场可以抑制TGF-β1 mRNA基因表达的下降,促进成骨细胞的分化.
목적 연구불동강도적자성부착체모의정자장대성골세포전화생장인자β1(TGF-β1)기인표체적영향.방법 이용세포정자장가재장치,대체외배양적SD대서성골세포분별진행12.5、125화250 mT적정자장가재,련속가재0.5、1、3、5화7d,채용반전록취합매련반응(RT-PCR)기술검측성골세포적TGF-β1 mRNA표체적.결과 12.5 mT자장가재조재가재0.5、1화3d,세포적TGF-βl mRNA적표체비대조조상응시단명현증강(P＜0.05).125화250 mT자장조가재정자장0.5、1、3、5화7d후,세포적TGF-β1 mRNA적표체승고경가명현,여대조조화12.5mT자장가재조세포상비차이유통계학의의(P＜0.05).정자장대세포적TGF-β1 mRNA표체적영향유일정적제량-효응관계.결론 재본시험조건하,자성부착체모의정자장가이억제TGF-β1 mRNA기인표체적하강,촉진성골세포적분화.
Objective To explore the effects of static magnetic fields (SMF) of dental magnetic attachment system with different intensities on the expression of TGF-β1 mRNA of rat osteoblasts.Methods The cultured rat osteoblasts were exposed continuously to 12.5 mT, 125 mT,and 250 mT SMF for 0.5,1,3,5,and 7 days,respectively.While,controls were also set up free of SMF.Realtime quantitative PCR was employed to detect the gene expression of cellular TGF-β1 mRNA and the statistical analysis was performed.Results Osteoblasts exposed to 12.5 mT SMF for 0.5,1,and 3 days exhibited an increase on cellular TGF-β1 mRNA expression,as compared to those of the control group (P ＜ 0.05).Moreover,after 0.5,1,3,5,and 7 days SMF exposure the cellular TGF-β1 mRNA expression in 125 mT and 250 mT groups were significantly higher than those of the control group and the 12.5 mT group (P ＜ 0.05).It showed a dose-effect relationship between the magnetic intensity and the expression of cellular TGF-β1 mRNA.Conclusions Under this experimental condition,the static magnetic fields of magnetic attachments can stimulate the expression of cellular TGF-β1 mRNA in rat osteoblasts,as to enchance the differentiation of osteoblasts.