CAJ | 학술논문

目的本实验将微囊化肝细胞移植人急性肝衰竭大鼠腹腔内,动态观察大鼠肝功能和肝组织中高迁移率族蛋白B1(HMGB1)的表达情况和意义. 方法用D-氨基半乳糖制作大鼠急性肝衰竭模型,随机分成3组:Ⅰ组为生理盐水组,Ⅱ组为裸肝细胞移植组,Ⅲ组为微囊化肝细胞移植组.在6、12、24、48、72、120和168 h,检测各组血生化,用RT-PCR方法检测肝组织HMGB1 mRNA的表达.另取6只正常大鼠样本作为参考值. 结果各模型组ALT、AST和TBil在6 h就开始上升,24 h均已显著升高(P＜0.05),Ⅲ组ALT、AST和TBil显著下降,与Ⅰ、Ⅱ组比较差异有统计学意义(P＜0.05或P＜0.01).各模型组HMGB1mRNA在6 h显著升高(P＜0.01),12 h达高峰,Ⅱ组、Ⅲ组在24 h后表达量较Ⅰ组显著下降,72 h、120 h时Ⅲ组与Ⅱ组比较差异有统计学意义(P＜0.01). 结论 HMGB1可能参与了急性肝衰竭的病理生理过程,微囊化肝细胞移植组明显减少了肝衰竭大鼠肝组织中HMGB1 mRNA的表达,从而减轻肝组织炎症反应并改善肝衰竭大鼠的预后.
목적 본실험장미낭화간세포이식인급성간쇠갈대서복강내,동태관찰대서간공능화간조직중고천이솔족단백B1(HMGB1)적표체정황화의의. 방법 용D-안기반유당제작대서급성간쇠갈모형,수궤분성3조:Ⅰ조위생리염수조,Ⅱ조위라간세포이식조,Ⅲ조위미낭화간세포이식조.재6、12、24、48、72、120화168 h,검측각조혈생화,용RT-PCR방법검측간조직HMGB1 mRNA적표체.령취6지정상대서양본작위삼고치. 결과 각모형조ALT、AST화TBil재6 h취개시상승,24 h균이현저승고(P＜0.05),Ⅲ조ALT、AST화TBil현저하강,여Ⅰ、Ⅱ조비교차이유통계학의의(P＜0.05혹P＜0.01).각모형조HMGB1mRNA재6 h현저승고(P＜0.01),12 h체고봉,Ⅱ조、Ⅲ조재24 h후표체량교Ⅰ조현저하강,72 h、120 h시Ⅲ조여Ⅱ조비교차이유통계학의의(P＜0.01). 결론 HMGB1가능삼여료급성간쇠갈적병리생리과정,미낭화간세포이식조명현감소료간쇠갈대서간조직중HMGB1 mRNA적표체,종이감경간조직염증반응병개선간쇠갈대서적예후.
Objective To detect hepatic function and high mobility group box 1(HMGB1)in dynamic state after microencapsulated hepatocytes transplating into rats with acute liver failure(ALF). Methods We made the model of ALF with D-GalN and assigned the models to 3 groups randomly: group Ⅰ were made with NS; group Ⅱ were made with gymno-hepatocyte; group Ⅲ were made with microencapsulated hepatocyte. The changes of hepatic function and HMGB1mRNA were detected by semiquantitative RT-PCR at the time of 6 h, 12 h, 24 h, 48 h, 72 h, 120 h and 168 h.In addition, reference value came from 6 normal rats. Results ALT, AST and total bilirubin(Tbil)began to increase at 6 h in 3 model groups, and increased significantly at the time of 24 h( P ＜ 0.05). ALT, AST and Tbil in group Ⅲ were decreased obviously, and there was statistical significance with group Ⅰ and group Ⅱ ( P ＜ 0.05 or P ＜ 0.01). In 3model groups, the expression of HMGB1 mRNA increased obviously at 6 h( P ＜ 0.01)and reached to peak at 12 h. Compared with group Ⅰ ,the expression of HMGB1 mRNA in groupⅡ and groupⅢ descended significantly after 24 h. There was statistical significance between group Ⅲ and group Ⅱ at 72 h and 120 h( P ＜ 0.01). Conclusions HMGB1 are intimately associated with ALF. Hepatocellular transplantation can decrease the expression of HMGB1 mRNA in hepatic tissue of rats with ALF, reduce the inflammation of the liver and improve the prognosis of ALF rats.