中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
11期
767-771
,共5页
薛旭红%吴宏斌%郑启新%李鹏%邹立学
薛旭紅%吳宏斌%鄭啟新%李鵬%鄒立學
설욱홍%오굉빈%정계신%리붕%추립학
创伤和损伤%软骨,关节%寡核苷酸序列分析%绵羊
創傷和損傷%軟骨,關節%寡覈苷痠序列分析%綿羊
창상화손상%연골,관절%과핵감산서렬분석%면양
Wounds and injuries%Cartilage,articular%Oligonucleotide array sequence analysis%Sheep
目的 探讨成年及幼年羊关节软骨对急性机械性损伤在分子水平的反应.方法 建立羊的不同发育阶段关节软骨组织机械性损伤体外模型,运用羊的全基因组表达谱芯片检测分析基因表达谱,并将所筛选的差异基因通过实时定量聚合酶链反应(PCR)验证.统计学分析采用方差分析.结果 创伤后成年羊及幼年羊组均有基因表达差异调节:幼年羊组有86个基因而成年羊组有83个基因(P<0.05);其中有25个基因为功能注释基因;显著差异功能基因包括过氧化物酶体增殖激活受体γ(PPARγ)、生长相关致癌基因(GRO)、腱糖蛋白C(TNC)及乳酸脱氢酶A(LDHA).结论 机械损伤后成幼年羊关节软骨均有明显的基因表达差异,显著差异功能基因包括与伤口愈合、发育、细胞外基质稳定和能量代谢等相关.
目的 探討成年及幼年羊關節軟骨對急性機械性損傷在分子水平的反應.方法 建立羊的不同髮育階段關節軟骨組織機械性損傷體外模型,運用羊的全基因組錶達譜芯片檢測分析基因錶達譜,併將所篩選的差異基因通過實時定量聚閤酶鏈反應(PCR)驗證.統計學分析採用方差分析.結果 創傷後成年羊及幼年羊組均有基因錶達差異調節:幼年羊組有86箇基因而成年羊組有83箇基因(P<0.05);其中有25箇基因為功能註釋基因;顯著差異功能基因包括過氧化物酶體增殖激活受體γ(PPARγ)、生長相關緻癌基因(GRO)、腱糖蛋白C(TNC)及乳痠脫氫酶A(LDHA).結論 機械損傷後成幼年羊關節軟骨均有明顯的基因錶達差異,顯著差異功能基因包括與傷口愈閤、髮育、細胞外基質穩定和能量代謝等相關.
목적 탐토성년급유년양관절연골대급성궤계성손상재분자수평적반응.방법 건립양적불동발육계단관절연골조직궤계성손상체외모형,운용양적전기인조표체보심편검측분석기인표체보,병장소사선적차이기인통과실시정량취합매련반응(PCR)험증.통계학분석채용방차분석.결과 창상후성년양급유년양조균유기인표체차이조절:유년양조유86개기인이성년양조유83개기인(P<0.05);기중유25개기인위공능주석기인;현저차이공능기인포괄과양화물매체증식격활수체γ(PPARγ)、생장상관치암기인(GRO)、건당단백C(TNC)급유산탈경매A(LDHA).결론 궤계손상후성유년양관절연골균유명현적기인표체차이,현저차이공능기인포괄여상구유합、발육、세포외기질은정화능량대사등상관.
Objective To investigate the molecular response of adult and neonate ovine articular cartilage to acute mechanical injury.Methods An established in vitro model was used to compare gene expression difference of ovine articular cartilage explants at different developmental stages 24 hours after mechanical injury and the gene expression was compared between these models and that of the uninjured controls by microarray analysis.Total RNA was isolated from the tissue samples,linearly amplified,and applied to a 15 208 Ovine probes cDNA microarray (Agilent).Validation for selected genes (PPARγ,GRO TNC and LDHA) was obtained by real-time polymerase chain reaction.Comparisons between groups were performed by variance analysis.Results There was significant difference in gene expression in adult and neonatal ovine articular cartilage after mechanical injury.Eighty-six genes were significantly manipulated at least 2-fold following mechanical injury for neonate sheep and 83 genes for adult sheep (P<0.05).Conclusion Our findings indicate that mechanical injury to adult and neonatal ovine articular cartilage results in the activation of a series of signaling responses.We could identify four significant genes that are up or downregulated in response to acute mechanical injury.Significant functional clusters including genes associated with wound healing,articular protection,repair integration,and energy metabolism.Of these,PPARG could be specifically identified as novel target molecules and potential chondroprotective agent involved in traumatic cartilage injury and cartilage integrated repair.