CAJ | 학술논문

目的观察钛合金颗粒对体外培养小鼠成骨细胞增殖以及核因子(NF) -κB受体活化因子配体(RANKL)的影响,探讨假体周围骨溶解机制.方法体外培养小鼠成骨细胞,随机分为空白对照组和钛合金颗粒处理A(0.01％)、B(0.10％)、C(1.00％)组.采用细胞计数试剂盒(CCK)-8法检测培养24、48、96、168 h成骨细胞增殖活性.分别采用逆转录-聚合酶链反应(RT-PCR)、酶联免疫吸附试验(ELISA)检测培养24、48 h后成骨细胞的RANKL mRNA表达及培养上清液的RANKL含量.结果培养48 h,A组、B组与对照组比较细胞增殖活性差异无统计学意义(P＞0.05),C组与其他各组比较细胞增殖活性明显降低(P＜0.01).培养168 h,B组细胞增殖活性下降,和对照组比较差异有统计学意义(P＜0.01).培养24、48 h,和对照组比较,各实验组RANKL mRNA表达及分泌量均明显升高(P＜0.01),各实验组之间差异有统计学意义(P＜0.01).结论钛合金颗粒可降低成骨细胞增殖活性,促进成骨细胞RANKL表达与分泌,是假体周围骨溶解机制之一.
목적 관찰태합금과립대체외배양소서성골세포증식이급핵인자(NF) -κB수체활화인자배체(RANKL)적영향,탐토가체주위골용해궤제.방법 체외배양소서성골세포,수궤분위공백대조조화태합금과립처리A(0.01％)、B(0.10％)、C(1.00％)조.채용세포계수시제합(CCK)-8법검측배양24、48、96、168 h성골세포증식활성.분별채용역전록-취합매련반응(RT-PCR)、매련면역흡부시험(ELISA)검측배양24、48 h후성골세포적RANKL mRNA표체급배양상청액적RANKL함량.결과 배양48 h,A조、B조여대조조비교세포증식활성차이무통계학의의(P＞0.05),C조여기타각조비교세포증식활성명현강저(P＜0.01).배양168 h,B조세포증식활성하강,화대조조비교차이유통계학의의(P＜0.01).배양24、48 h,화대조조비교,각실험조RANKL mRNA표체급분비량균명현승고(P＜0.01),각실험조지간차이유통계학의의(P＜0.01).결론 태합금과립가강저성골세포증식활성,촉진성골세포RANKL표체여분비,시가체주위골용해궤제지일.
Objective To observe the effect of titanium alloy particles on the cell proliferation and the expression of gene receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) in osteoblasts of mice,and to investigate the mechanism of periprosthetic osteolysis.Methods Osteoblasts were cultured in 10％ serum medium containing different concentrations of titanium alloy particles.The cells were randomly divided into four groups.In the experimental groups A,B and C,osteoblasts were cultured with 0.01％,0.10％ and 1.00％ concentrations of titanium alloy particles,respectively; in the control group,no titanium alloy particles were given.Cell Counting Kit-8 (CCK-8) test was used to assess the cell proliferation at 24,48,96,168 h,respectively.At 24 and 48 h,reverse transcription-polymerase chain reaction (RTPCR) and enzyme linked immunosorbent assay (ELISA) were performed to detect the RANKL expression in osteoblasts and the intensity of the concentrations of RANKL in the supernatant of medium,respectively.Results The proliferation of osteoblasts had no differences between experimental groups A or B and control group at 48 h (P ＞ 0.05).The proliferation of osteoblasts in the experimental group C was significantly reduced as compared with other groups at 48 h (P ＜ 0.01 ).Meanwhile the titanium alloy particles up-regulated the mRNA and protein expression of RANKL ( P ＜ 0.01 ).Conclusion The proliferation of osteoblasts is decreased with exposure to titanium particles and different concentrations of titanium alloy particles could up-regulate the mRNA and protein expression of RANKL,which may be one of the mechamisms for the periprosthetic osteolysis.