CAJ | 학술논문

目的探讨AP3B型接合蛋白复合体μ亚基基因敲除小鼠(AP3M2KO小鼠)的(癎)性发作特征及机制.方法建立和繁殖AP3M2KO小鼠,利用远隔摄像和无线脑电图监测系统对小鼠的(癎)性发作表现及脑电图变化进行观测.利用脑内微小透析法对小鼠脑内神经递质的变化进行探讨.结果 AP3M2KO小鼠在生后8周开始出现自发性痉挛症状.发作时小鼠脑颞叶脑电图出现典型的(癎)性波群.与野生小鼠相比,AP3M2KO小鼠海马神经细胞谷氨酸的基础及钾离子刺激性释放[分别为(0.35±0.08)pmol/20μl和(0.72±0.25)pmol/20μl],γ-氨基丁酸(GABA)的基础释放[(2.94±1.69)fmol/20μl]皆未见明显差异;GABA的钾离子刺激性释放[(63.5±11.8)fmol/20μl]明显减少(t=4.405,P＜0.05).结论 AP3M2KO小鼠的(癎)性发作与人类癫(癎)发作的临床表现相类似,其(癎)性发作机制可能与GABA抑制性神经传递系统的功能低下有关.
목적 탐토AP3B형접합단백복합체μ아기기인고제소서(AP3M2KO소서)적(간)성발작특정급궤제.방법 건립화번식AP3M2KO소서,이용원격섭상화무선뇌전도감측계통대소서적(간)성발작표현급뇌전도변화진행관측.이용뇌내미소투석법대소서뇌내신경체질적변화진행탐토.결과 AP3M2KO소서재생후8주개시출현자발성경련증상.발작시소서뇌섭협뇌전도출현전형적(간)성파군.여야생소서상비,AP3M2KO소서해마신경세포곡안산적기출급갑리자자격성석방[분별위(0.35±0.08)pmol/20μl화(0.72±0.25)pmol/20μl],γ-안기정산(GABA)적기출석방[(2.94±1.69)fmol/20μl]개미견명현차이;GABA적갑리자자격성석방[(63.5±11.8)fmol/20μl]명현감소(t=4.405,P＜0.05).결론 AP3M2KO소서적(간)성발작여인류전(간)발작적림상표현상유사,기(간)성발작궤제가능여GABA억제성신경전체계통적공능저하유관.
Objective To explore the mechanism of spontaneous seizures in adaptor protein complex type 3B knockout mice(AP3M2KO mice).Methods AP3M2KO mice were generated.Seizures and electroencephalogram(EEG)were monitored using video camera and telemetry system.Glutamate and GABA releases were determined using in vivo microdialysis method.Results AP3M2KO mice began to suffer from spontaneous seizures 8 weeks after the birth,but did not show any other behavior abnormality.The onset of ictal discharge over the temporal region was synchronized with seizures.There were no significant differences in basal glutamate and GABA releases in hippocampus between AP3M2KO((0.35±0.08)pmol/20μl and(2.94±1.69)fmol/20μl,respectively)and wild-type mice.However,the 50 mmol/L K+-evoked GABA release was impaired in AP3M2KO mice((63.5±11.8)fmol/20μl vs(209.2±63.7)fmol/20 μl,t=4.405,P＜0.05),whereas no significant difference was found in K+-evoked glutamate release.Conclusions AP3M2KO mice suffer from epileptic seizures similar to the clinical features of human epilepsy.The impairment of inhibitory GABAergic transmission iS involved in the mechanism of spontaneous seizures in AP3M2KO mice.