CAJ | 학술논문

目的探讨肺移植无心跳供者气管上皮耐受热缺血的时限.方法将SD大鼠分为4组.对照组在大鼠有心跳的情况下取出气管,热缺血30 min组、45 min组和60 min组分别在处死大鼠后30、45和60 min时取出气管,然后将供者气管固定后分别植入相应受者的大网膜内.14 d后,取出气管,进行病理学检查,并对上皮再生情况进行评分.结果热缺血30min组和对照组比较,无论是上皮厚度还是黏膜上皮细胞层数的差异均无统计学意义(P>0.05);虽然45 min组与对照组相比,上皮厚度的差异有统计学意义(P<0.05),但45 min组的气管上皮有正常黏膜结构,并且与对照组相比,上皮细胞层数差异无统计学意义(P<0.05);而热缺血60min组,看不到正常的上皮结构,上皮也仅由1～2层黏膜上皮细胞覆盖.结论气管耐受热缺血的时限可能为45 min.
목적 탐토폐이식무심도공자기관상피내수열결혈적시한.방법 장SD대서분위4조.대조조재대서유심도적정황하취출기관,열결혈30 min조、45 min조화60 min조분별재처사대서후30、45화60 min시취출기관,연후장공자기관고정후분별식입상응수자적대망막내.14 d후,취출기관,진행병이학검사,병대상피재생정황진행평분.결과 열결혈30min조화대조조비교,무론시상피후도환시점막상피세포층수적차이균무통계학의의(P>0.05);수연45 min조여대조조상비,상피후도적차이유통계학의의(P<0.05),단45 min조적기관상피유정상점막결구,병차여대조조상비,상피세포층수차이무통계학의의(P<0.05);이열결혈60min조,간불도정상적상피결구,상피야부유1～2층점막상피세포복개.결론 기관내수열결혈적시한가능위45 min.
Objective To determine the length of warm ischemic (WI) tolerance in bronchial graft from non-heart-beating donors. Methods Forty-eight rats were randomly divided into 4 groups (each group having 12 rats) according to different WI durations including WI-0 min (group A), WI-30 min (group B), WI-45 min (group C) and WI-60 min (group D). In each group, the tracheae from 6 rats were respectively imbedded in greater omentum of other 6 rats, and 14 days later, the transplanted tracheae were taken from recipients to evaluate epithelial thickness and regeneration. Results Epithelial thickness and the degree of epithelial regeneration had no significant difference (P >0. 05) between the syngeneic control group and the WI-30 minutes group. All of the grafts with WI duration of 45 min were viable, but the epithelium was significantly thinner than that in the syngeneic control group (P<0. 05). However all of the grafts with WI duration of 60 min showed lower viability rate. Conclusion The time limits of tolerance to WI of tracheal grafts from NHBDs may be 45 min.