CAJ | 학술논문

目的检测Bcl-w在大肠癌中的表达,了解Bcl-w与临床重要病理因子的关系;研究Bcl-w与Bcl-2基因及其P53基因的关系;探讨Bcl-w表达对肿瘤细胞凋亡的影响.方法免疫组化法检测Bcl-w,Bcl-2及其P53在大肠癌中的表达;Western blot进一步定量检测Bcl-w在大肠癌中的表达;TUNEL检测大肠癌中的细胞凋亡情况.结果全组52例病人中,39例Bcl-w表达阳性,占75%.随肿瘤浸润深度增加,淋巴转移,肝转移,Bcl-w表达亦增加.3年随访中,Bcl-w表达阳性大肠癌患者均死亡.与Bcl-2相反,Bcl-w在大肠腺瘤中并不表达.Bcl-w与P53成显著正相关,Bcl-w与肿瘤细胞凋亡呈显著负相关.结论 Bcl-w与大肠癌的浸润与转移密切相关.它可能是大肠癌预后相关因素之一.与Bcl-2不同,Bcl-w在肿瘤进展阶段起重要作用.此外,Bcl-w在大肠癌细胞中起到抗凋亡作用,P53基因下调Bcl-w的表达.
목적 검측Bcl-w재대장암중적표체,료해Bcl-w여림상중요병리인자적관계;연구Bcl-w여Bcl-2기인급기P53기인적관계;탐토Bcl-w표체대종류세포조망적영향.방법 면역조화법검측Bcl-w,Bcl-2급기P53재대장암중적표체;Western blot진일보정량검측Bcl-w재대장암중적표체;TUNEL검측대장암중적세포조망정황.결과 전조52례병인중,39례Bcl-w표체양성,점75%.수종류침윤심도증가,림파전이,간전이,Bcl-w표체역증가.3년수방중,Bcl-w표체양성대장암환자균사망.여Bcl-2상반,Bcl-w재대장선류중병불표체.Bcl-w여P53성현저정상관,Bcl-w여종류세포조망정현저부상관.결론 Bcl-w여대장암적침윤여전이밀절상관.타가능시대장암예후상관인소지일.여Bcl-2불동,Bcl-w재종류진전계단기중요작용.차외,Bcl-w재대장암세포중기도항조망작용,P53기인하조Bcl-w적표체.
[Objective] To investigate Bcl-w expression and its relationship with clinical pathologic factors in colorectal carcinoma. The effect of Bcl-w expression on the apoptosis and the relationship with Bcl-2 and P53 expression were also detected in these tumors.[Methods] The expression of Bel-w, Bel-2 and P53 was studied in 52 colorectal carcinoma tissue samples by using immunohistochemistry. Bel-w expression was further assessed with Western blot. The function of Bcl-w expression on the apoptosis of colorectal carcinoma was detected by TUNEL. [Results] Positive immunoreactivity was identified of Bcl-w in 39 of 52(75%) colorectal cancer specimens. The expression level of Bcl-w was increased with tumor's invasion, lymph node and liver metastasis.Patients with tumor expressed Bcl-w were all died within 3 years after surgery. In contrast to Bcl-2, colorectal adenoma had not expressed Bcl-w, the statistics analysis revealed a significant negative correlation between the Bcl-w and apoptosis and a clear inverse relationship between P53 and Bcl-w expression.[Conclutions] The expression of Bcl-w is significantly related to the progression of the colorectal cancer. It could be regarded as a prognostic factor for this cancer. In contrast to Bcl-2, Bcl-w play an important role in the advanced tumor stage. Furthermore, Bcl-w is a potent suppressor for apoptosis in colorectal carcinoma. An effect of P53 on down-regulating Bel-w expression is also demonstrated.