东北农业大学学报
東北農業大學學報
동북농업대학학보
JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY
2009年
10期
75-78
,共4页
野交杂种猪%NRAMP1基因%定量PCR
野交雜種豬%NRAMP1基因%定量PCR
야교잡충저%NRAMP1기인%정량PCR
wild boar hybrid%NRAWP1 gene%quantitative PCR
采用Real time PCR方法对野交杂种猪的NRA MP1基因进行了研究.结果表明,在大脑、肺脏、淋巴结、白细胞和脾脏组织出现强的扩增,在肝脏、回肠、肾脏、肌肉、心脏出现弱的扩增.Real time PCR的结果证实该基因在不同组织中的表达量都有差异,其中在肾脏中表达的最高,在肝脏中表达最低,两者间相差71倍,从高到低依次是脾脏、大脑、肺脏、回肠、肾脏、淋巴结、结肠、心脏、肌肉和肝脏.
採用Real time PCR方法對野交雜種豬的NRA MP1基因進行瞭研究.結果錶明,在大腦、肺髒、淋巴結、白細胞和脾髒組織齣現彊的擴增,在肝髒、迴腸、腎髒、肌肉、心髒齣現弱的擴增.Real time PCR的結果證實該基因在不同組織中的錶達量都有差異,其中在腎髒中錶達的最高,在肝髒中錶達最低,兩者間相差71倍,從高到低依次是脾髒、大腦、肺髒、迴腸、腎髒、淋巴結、結腸、心髒、肌肉和肝髒.
채용Real time PCR방법대야교잡충저적NRA MP1기인진행료연구.결과표명,재대뇌、폐장、림파결、백세포화비장조직출현강적확증,재간장、회장、신장、기육、심장출현약적확증.Real time PCR적결과증실해기인재불동조직중적표체량도유차이,기중재신장중표체적최고,재간장중표체최저,량자간상차71배,종고도저의차시비장、대뇌、폐장、회장、신장、림파결、결장、심장、기육화간장.
Using semi-quantitative and quantitative PCR methods on hybrid wild boar, such as NRAMP1 gene in 10 tissues were studied. The results of semi-quantitative showed that in brain, lung, lymph nodes, leukocytes and spleen tissue appeared strong amplification; in liver, ileum, kidney, muscle and heart appeared weak amplification. The results of quantitative PCR confirmed that the genes in different tissues vary the volume, the maximal expression was observed in spleen and the minimum in liver. The difference between the maximal and the minimum was more than 71 times. Which followed by that of low spleen, brain, lung, ileum, kidney, lymph nodes, colon, heart, muscle and liver.
