CAJ | 학술논문

从采集到的污水样品中分离得到1株高效降解2-甲基异茨醇(2-methylisoborneol,2-MIB)的菌株Z5,通过16S rDNA序列分析,鉴定为阴沟肠杆菌(Enterobacter cloacae).该菌在以2-MIB为唯一碳源的培养基中能正常生长代谢.经气相色谱-质谱分析,发现该菌对2-MIB的降解率高达89.7%.同时,测定了Z5在不同质量浓度2-MIB中的生长情况,结果表明:当2-MIB质量浓度为16μg/L时,其生长状况与在LB中最为接近.为了克隆降解2-MIB关键酶的编码基因并进一步研究其降解途径,本研究构建了E.cloacae Z5的全基因组BAC文库,该文库覆盖了大约8倍的基因组,共计810个转化子,外源片段平均大小为40 kb.
종채집도적오수양품중분리득도1주고효강해2-갑기이자순(2-methylisoborneol,2-MIB)적균주Z5,통과16S rDNA서렬분석,감정위음구장간균(Enterobacter cloacae).해균재이2-MIB위유일탄원적배양기중능정상생장대사.경기상색보-질보분석,발현해균대2-MIB적강해솔고체89.7%.동시,측정료Z5재불동질량농도2-MIB중적생장정황,결과표명:당2-MIB질량농도위16μg/L시,기생장상황여재LB중최위접근.위료극륭강해2-MIB관건매적편마기인병진일보연구기강해도경,본연구구건료E.cloacae Z5적전기인조BAC문고,해문고복개료대약8배적기인조,공계810개전화자,외원편단평균대소위40 kb.
A bacterium strain Z5,which can degrade 2-methylisoborneol efficiently,was isolated from sewage samples collected from the sewage treatment plant of Wuhan.This strain was identified as Enterobacter cloacae via the analysis of 16S rDNA.The results of the sole carbon source assay demonstrated that this stain was able to metabolize 2-MIB and the degradation rate was up to 89.7% determined by GC-MS.The growth of E.cloacae Z5 in 2-MIB at different concentrations were also determined and the growth curve in 16 μg/L 2-MIB was found to be as good as that in LB.To clone the gene coding for the key enzyme catalyzing the degradation of 2-MIB and to further investigate the degradation pathway,the global genome BAC library of E.cloacae Z5 was constructed.There were 810 clones in the library.The average size of inserts and the genome coverage was 40 kb and about 8-folds,respectively.