CAJ | 학술논문

背景:单纯的神经干细胞移植对受损脊髓组织的修复作用并不理想,研究证实神经生长因子兼有神经元营养和促突起生长双重作用,可以有效的促进脊髓损伤后神经功能的恢复.目的:观察神经干细胞移植联合应用神经生长因子对脊髓损伤后大鼠运动功能恢复的影响.方法:SD大鼠42只,建立急性脊髓损伤模型后随机分成3组,伤后1周于损伤处分别注入培养液、单纯神经干细胞或神经干细胞联合神经生长因子.于伤后1,2,4,6,8周进行BBB评分和斜板实验等运动功能检测.伤后4周取材行病理切片苏木精-伊红染色及BrdU免疫组化染色,伤后8周取材行辣根过氧化物酶示踪观察及体感诱发电位观察神经电生理恢复情况.结果与结论:伤后4周单纯神经干细胞组、神经于细胞联合神经生长因子组大鼠后肢运动功能均有较明显恢复,神经干细胞联合神经生长因子组较单纯神经干细胞组快,差异有显著性意义(P＜0.05).培养液组亦有所恢复,但程度较轻.病理切片显示培养液组未见神经轴索通过.单纯神经干细胞组可见少量神经轴索样结构,神经干细胞联合神经生长因子组可见较多神经轴索样结构.BrdU的阳性细胞数及HRP阳性神经纤维数:神经干细胞联合神经生长因子组＞单纯神经干细胞组＞培养液组且各组之间差异有显著性意义(P＜0.01).神经干细胞联合神经生长因子组大鼠体感诱发电位的潜伏期、波幅优于单纯神经干细胞组(P＜0.05),明显优于培养液组(P＜0.01). 结果提示神经干细胞移植对于后肢功能的恢复有促进作用,联合应用神经生长因子有协同效果.
배경:단순적신경간세포이식대수손척수조직적수복작용병불이상,연구증실신경생장인자겸유신경원영양화촉돌기생장쌍중작용,가이유효적촉진척수손상후신경공능적회복.목적:관찰신경간세포이식연합응용신경생장인자대척수손상후대서운동공능회복적영향.방법:SD대서42지,건립급성척수손상모형후수궤분성3조,상후1주우손상처분별주입배양액、단순신경간세포혹신경간세포연합신경생장인자.우상후1,2,4,6,8주진행BBB평분화사판실험등운동공능검측.상후4주취재행병리절편소목정-이홍염색급BrdU면역조화염색,상후8주취재행랄근과양화물매시종관찰급체감유발전위관찰신경전생리회복정황.결과여결론:상후4주단순신경간세포조、신경우세포연합신경생장인자조대서후지운동공능균유교명현회복,신경간세포연합신경생장인자조교단순신경간세포조쾌,차이유현저성의의(P＜0.05).배양액조역유소회복,단정도교경.병리절편현시배양액조미견신경축색통과.단순신경간세포조가견소량신경축색양결구,신경간세포연합신경생장인자조가견교다신경축색양결구.BrdU적양성세포수급HRP양성신경섬유수:신경간세포연합신경생장인자조＞단순신경간세포조＞배양액조차각조지간차이유현저성의의(P＜0.01).신경간세포연합신경생장인자조대서체감유발전위적잠복기、파폭우우단순신경간세포조(P＜0.05),명현우우배양액조(P＜0.01). 결과제시신경간세포이식대우후지공능적회복유촉진작용,연합응용신경생장인자유협동효과.
BACKGROUND:The effects of simple neural stem cell(NSC)transplantation on repair of damaged spinal cord are not ideal.Previous studies have shown that newa growth factor(NGF)has both effects of neuron nutrition and process growth promotion,can effectively contribute to the recovery of neurofunction following spinal cord injury(SCI).OBJECTIVE:To investigate the effect of neuraI sterR cell transplantation combined with NGF application on the recovery of motor function of ratswith SCI.METHODS:A total of 42 Sprague Dawley rats were used to establish SPI models.and then divided randomly into thtee groups:medium,simple NSCs or NSCs+NGF.At 1 week foliowing injury,medium,simple NSCs or NSCs+NGF were separately injected into damaged sites.At 1,2,4,6.8 weeks post-injury.all animals were evaluated on the hind limb behavior with Basso.Beattie and Bresnahan(BBB)locomotor rating scale and inclined plane test.At 4 weeks post-transplantation,histopathology hematoxylin-eosin staining and BrdU immunohistochemistry were performed.At 8 weeks post-transplantation.horseradish peroxidase(HRP)nerve trace and somatosensory evoked potential testing were performed to observe the recovery of nerve electrophysiology.RESULTS AND CONCLUSION:At 4 weeks fo,owing injury.motor function of the rat hindlimb was significantly improved in the simple NSCs group and NSCs+NGF group.The recovery was rapid in the NSCs+NGF group than the simple NSCs group(P＜0.05).The recovery was slight in the medium group.Pathological sections demonstrated that neurite was not found in the medium group.A few neurites were seen in the simple NSCs group.and many neurites were observed in the NSCs+NGF group.Numbers of BrdU-positive cells and HRP-positive nerve fibers:NSCs+NGF group＞simple NSCs group＞medium group there was significant difference among groups(P＜0.01).Latent period and amplitude of somatosensory evoked potentials in the NSCs+NGF group were superior to the simple NSCs group(P＜0.05),and significantly better than the medium group(P＜0.01).Results suggested that NSC transplantation can promote the recovery of hindlimb function,and combined with NGF presents synergic effect.