中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2008年
3期
276-279
,共4页
耿明%尹迎春%曹永成%付质杰%邰艳红
耿明%尹迎春%曹永成%付質傑%邰豔紅
경명%윤영춘%조영성%부질걸%태염홍
胃肿瘤%基因,Bcl-2%化疗药物%敏感性%免疫组织化学
胃腫瘤%基因,Bcl-2%化療藥物%敏感性%免疫組織化學
위종류%기인,Bcl-2%화료약물%민감성%면역조직화학
Stomach neoplasms%Genes,Bcl-2%Chemotherapeutic%Sensitivity%Immunohistochemistry
目的 观察化疗药物对原代胃癌细胞的体外杀伤作用,探讨其与胃癌组织中Bcl-2蛋白表达的关系.方法 新鲜胃癌组织制备单细胞悬液,分别加入紫杉醇(Tax)、顺铂(DDP)、阿霉素(ADM)、氟尿嘧啶(5-Fu)和丝裂霉素(MMC)培养48 h.四氮唑盐还原法(MTr)观察药物作用后癌细胞活力及代谢活性变化.免疫组织化学技术检测Bcl-2的表达.结果 5种化疗药物平均抑制率依次为Tax(40.6±6.9)%、5-FU(38.9±9.2)%、CDDP(38.4±7.8)%、ADM(31.6±8.5)%、MMC(28.9±9.8)%.不同类型胃癌对5种化疗药物的敏感性由强到弱依次为印戒细胞癌、管状腺癌、黏液腺癌和乳头状腺癌.全组Bcl-2阳性率为80%,阳性者对5-Fu、MMC和ADM有较强的耐药性.结论 MTY比色法体外药敏试验,有助于筛选个体化有效治疗药物.Bcl-2的高表达可能是胃癌多药耐药的原因之一.
目的 觀察化療藥物對原代胃癌細胞的體外殺傷作用,探討其與胃癌組織中Bcl-2蛋白錶達的關繫.方法 新鮮胃癌組織製備單細胞懸液,分彆加入紫杉醇(Tax)、順鉑(DDP)、阿黴素(ADM)、氟尿嘧啶(5-Fu)和絲裂黴素(MMC)培養48 h.四氮唑鹽還原法(MTr)觀察藥物作用後癌細胞活力及代謝活性變化.免疫組織化學技術檢測Bcl-2的錶達.結果 5種化療藥物平均抑製率依次為Tax(40.6±6.9)%、5-FU(38.9±9.2)%、CDDP(38.4±7.8)%、ADM(31.6±8.5)%、MMC(28.9±9.8)%.不同類型胃癌對5種化療藥物的敏感性由彊到弱依次為印戒細胞癌、管狀腺癌、黏液腺癌和乳頭狀腺癌.全組Bcl-2暘性率為80%,暘性者對5-Fu、MMC和ADM有較彊的耐藥性.結論 MTY比色法體外藥敏試驗,有助于篩選箇體化有效治療藥物.Bcl-2的高錶達可能是胃癌多藥耐藥的原因之一.
목적 관찰화료약물대원대위암세포적체외살상작용,탐토기여위암조직중Bcl-2단백표체적관계.방법 신선위암조직제비단세포현액,분별가입자삼순(Tax)、순박(DDP)、아매소(ADM)、불뇨밀정(5-Fu)화사렬매소(MMC)배양48 h.사담서염환원법(MTr)관찰약물작용후암세포활력급대사활성변화.면역조직화학기술검측Bcl-2적표체.결과 5충화료약물평균억제솔의차위Tax(40.6±6.9)%、5-FU(38.9±9.2)%、CDDP(38.4±7.8)%、ADM(31.6±8.5)%、MMC(28.9±9.8)%.불동류형위암대5충화료약물적민감성유강도약의차위인계세포암、관상선암、점액선암화유두상선암.전조Bcl-2양성솔위80%,양성자대5-Fu、MMC화ADM유교강적내약성.결론 MTY비색법체외약민시험,유조우사선개체화유효치료약물.Bcl-2적고표체가능시위암다약내약적원인지일.
Objective To evaluate in vitro anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells, and investigate the relationship with Bcl-2 expression. Methods Single cell suspension was prepared from fresh gastric cancer tissue and exposed to taxol (Tax), 5-fluorouracil (5-FU), eisplatin (CDDP), adriamyein (ADM), mitomycin (MMC) respectively for 48 hours. Metabolic activity and inhibitory rate of cells were detected by MTT assay. Expression of Bcl-2 was examined with immunohistoehemistry. Results The inhibitory rates of cancer cells exposed to chemotherapeutic drugs were different and Tax, 5-FU, CDDP had remarkahely higher rates than ADM and MMC. The lower differentiated gastric cancer cells were more sensitive than the higher ones. Positive expression rate of Bcl-2 was 80% and the positive cells showed resistance to 5-FU, ADM and MMC. Conclusions Chemosensitive testing by MTT assay can constitute the prediction for the application of chemotherapeutic drugs individually. Overexpression of Bcl-2 may contribute to multiple drug-resistance of tumors.