中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
3期
225-227
,共3页
徐乃玉%张素萍%聂继华%丁建松%童建
徐迺玉%張素萍%聶繼華%丁建鬆%童建
서내옥%장소평%섭계화%정건송%동건
氡%肺%蛋白质组
氡%肺%蛋白質組
동%폐%단백질조
Radon%Lung%Proteome
目的 从蛋白质组学角度分析氡染毒后大鼠肺组织蛋白质表达谱的变化.方法 雄性Wismr大鼠氡吸入染毒累积剂量分别达100、200、400工作水平月(WLM)后,取大鼠肺组织,裂解提取其总蛋白;双向电泳(2-DE)分离总蛋白,lmageMaster 2D Platinum软件分析差异表达蛋白,切取差异蛋白点,胶内酶解并进行质谱(MALDI-TOF-MS)鉴定.结果 正常对照组和氡染毒组大鼠肺组织的2-DE图谱有明显差异,差异表达点中与氡染毒呈剂量变化关系的点有14个表达上调,9个表达下调,质谱鉴定出其中的15个差异蛋白点.结论 氡染毒组大鼠肺组织的蛋白表达谱发生了一定的差异性,氡吸入致靶器官肺损伤可能与多种蛋白相关
目的 從蛋白質組學角度分析氡染毒後大鼠肺組織蛋白質錶達譜的變化.方法 雄性Wismr大鼠氡吸入染毒纍積劑量分彆達100、200、400工作水平月(WLM)後,取大鼠肺組織,裂解提取其總蛋白;雙嚮電泳(2-DE)分離總蛋白,lmageMaster 2D Platinum軟件分析差異錶達蛋白,切取差異蛋白點,膠內酶解併進行質譜(MALDI-TOF-MS)鑒定.結果 正常對照組和氡染毒組大鼠肺組織的2-DE圖譜有明顯差異,差異錶達點中與氡染毒呈劑量變化關繫的點有14箇錶達上調,9箇錶達下調,質譜鑒定齣其中的15箇差異蛋白點.結論 氡染毒組大鼠肺組織的蛋白錶達譜髮生瞭一定的差異性,氡吸入緻靶器官肺損傷可能與多種蛋白相關
목적 종단백질조학각도분석동염독후대서폐조직단백질표체보적변화.방법 웅성Wismr대서동흡입염독루적제량분별체100、200、400공작수평월(WLM)후,취대서폐조직,렬해제취기총단백;쌍향전영(2-DE)분리총단백,lmageMaster 2D Platinum연건분석차이표체단백,절취차이단백점,효내매해병진행질보(MALDI-TOF-MS)감정.결과 정상대조조화동염독조대서폐조직적2-DE도보유명현차이,차이표체점중여동염독정제량변화관계적점유14개표체상조,9개표체하조,질보감정출기중적15개차이단백점.결론 동염독조대서폐조직적단백표체보발생료일정적차이성,동흡입치파기관폐손상가능여다충단백상관
Objective To detect the changes in protein profile of rats'lung inhaled radon using two- dimensional electrophoresis(2-DE)and MS.Methods Male Wistar rats were exposed to radon with the cumulative dose up to 100,200 and 400 working level months(WLM).The total proteins of rats'lung were extracted and isolated by 2-DE.The different protein expressions were analyzed with ImageMaster 2D Platinum software and obviously altered proteins were digested by trypsin and identified by MALDI-TOF-MS.Resuits Comparison of the 2-DE images between the control and radon-exposed groups indicated 14 up-regulated and 9 down-regulated protein spots,15 of which were identified by MALDI-TOF or MALDI-TOF/TOF MS.Conclusions The 2-DE image of radon groups'lung has changed,and the lung damage effect of radon is related to some proteins.