中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2012年
1期
32-36
,共5页
邵贵强%曹秀琴%周玮丽%沈佳伟%史莉华%宋金波%陆海生
邵貴彊%曹秀琴%週瑋麗%瀋佳偉%史莉華%宋金波%陸海生
소귀강%조수금%주위려%침가위%사리화%송금파%륙해생
高压氧%2型糖尿病%葡萄糖转运蛋白%动态血糖监测
高壓氧%2型糖尿病%葡萄糖轉運蛋白%動態血糖鑑測
고압양%2형당뇨병%포도당전운단백%동태혈당감측
Hyperbaric oxygen%Type Ⅱ diabetes mellitus%Glucose transport protein%Dynamic glucose monitoring
目的 观察高压氧(hyperbaric oxygen,HBO)对2型糖尿病GK大鼠的降糖作用并探讨其作用机制.方法 选取5个月龄SPF级雄性GK大鼠72只(空腹血糖均在16.7 mmol/L以上),采用数字表法随机分为3组,即模型组、二甲双胍组、HBO组,每组再分成3个亚组,每组均为8只.另取24只Wistar大鼠为正常对照组,每次取8只用于3个不同的实验.HBO组每天行HBO治疗:0.15 MPa纯氧,稳压吸氧30 min;二甲双胍组按250 mg/(kg·d)用二甲双胍混悬液灌胃；正常对照组、模型组、HBO组每天按5 ml/(kg·d)灌服纯净水.1～3周后测定空腹血糖、葡萄糖转运蛋白含量.取1只Wistar大鼠、2只HBO组大鼠进行72 h血糖动态监测.所有大鼠隔日测定禁食4h后血糖,每组取2只大鼠电镜下观察胰腺组织中胰岛B细胞超微结构.结果 GK大鼠二甲双胍组第7天血糖为(9.71 ±2.07)mmol/L,HBO组第7天血糖为(9.79±2.12 )mmol/L,模型组血糖为(9.99±2.31) mmol/L,对照组血糖为(4.90±0.56) mmol/L,各组空腹血糖值比较差异无统计学意义(P＞0.05)；治疗第7天时,对照组血清葡萄糖转运蛋白浓度为(7.12 ±2.11) ng/L,模型组为(3.98±1.25) ng/L,二甲双胍组为(4.56±1.76) ng/L,HBO组为(4.37 ±0.68) ng/L,各组比较差异有统计学意义(P＜0.01)；治疗第14天时,对照组血清葡萄糖转运蛋白浓度为(7.06 ±0.54) ng/L,模型组为(3.99 ±0.85) ng/L,二甲双胍组为(5.06±0.77)ng/L,对照组与模型组、二甲双胍组比较差异有统计学意义(P＜0.01).结论 HBO可能通过提高葡萄糖转运蛋白水平、改善胰岛B细胞结构与功能而起到降低血糖的作用.
目的 觀察高壓氧(hyperbaric oxygen,HBO)對2型糖尿病GK大鼠的降糖作用併探討其作用機製.方法 選取5箇月齡SPF級雄性GK大鼠72隻(空腹血糖均在16.7 mmol/L以上),採用數字錶法隨機分為3組,即模型組、二甲雙胍組、HBO組,每組再分成3箇亞組,每組均為8隻.另取24隻Wistar大鼠為正常對照組,每次取8隻用于3箇不同的實驗.HBO組每天行HBO治療:0.15 MPa純氧,穩壓吸氧30 min;二甲雙胍組按250 mg/(kg·d)用二甲雙胍混懸液灌胃；正常對照組、模型組、HBO組每天按5 ml/(kg·d)灌服純淨水.1～3週後測定空腹血糖、葡萄糖轉運蛋白含量.取1隻Wistar大鼠、2隻HBO組大鼠進行72 h血糖動態鑑測.所有大鼠隔日測定禁食4h後血糖,每組取2隻大鼠電鏡下觀察胰腺組織中胰島B細胞超微結構.結果 GK大鼠二甲雙胍組第7天血糖為(9.71 ±2.07)mmol/L,HBO組第7天血糖為(9.79±2.12 )mmol/L,模型組血糖為(9.99±2.31) mmol/L,對照組血糖為(4.90±0.56) mmol/L,各組空腹血糖值比較差異無統計學意義(P＞0.05)；治療第7天時,對照組血清葡萄糖轉運蛋白濃度為(7.12 ±2.11) ng/L,模型組為(3.98±1.25) ng/L,二甲雙胍組為(4.56±1.76) ng/L,HBO組為(4.37 ±0.68) ng/L,各組比較差異有統計學意義(P＜0.01)；治療第14天時,對照組血清葡萄糖轉運蛋白濃度為(7.06 ±0.54) ng/L,模型組為(3.99 ±0.85) ng/L,二甲雙胍組為(5.06±0.77)ng/L,對照組與模型組、二甲雙胍組比較差異有統計學意義(P＜0.01).結論 HBO可能通過提高葡萄糖轉運蛋白水平、改善胰島B細胞結構與功能而起到降低血糖的作用.
목적 관찰고압양(hyperbaric oxygen,HBO)대2형당뇨병GK대서적강당작용병탐토기작용궤제.방법 선취5개월령SPF급웅성GK대서72지(공복혈당균재16.7 mmol/L이상),채용수자표법수궤분위3조,즉모형조、이갑쌍고조、HBO조,매조재분성3개아조,매조균위8지.령취24지Wistar대서위정상대조조,매차취8지용우3개불동적실험.HBO조매천행HBO치료:0.15 MPa순양,은압흡양30 min;이갑쌍고조안250 mg/(kg·d)용이갑쌍고혼현액관위；정상대조조、모형조、HBO조매천안5 ml/(kg·d)관복순정수.1～3주후측정공복혈당、포도당전운단백함량.취1지Wistar대서、2지HBO조대서진행72 h혈당동태감측.소유대서격일측정금식4h후혈당,매조취2지대서전경하관찰이선조직중이도B세포초미결구.결과 GK대서이갑쌍고조제7천혈당위(9.71 ±2.07)mmol/L,HBO조제7천혈당위(9.79±2.12 )mmol/L,모형조혈당위(9.99±2.31) mmol/L,대조조혈당위(4.90±0.56) mmol/L,각조공복혈당치비교차이무통계학의의(P＞0.05)；치료제7천시,대조조혈청포도당전운단백농도위(7.12 ±2.11) ng/L,모형조위(3.98±1.25) ng/L,이갑쌍고조위(4.56±1.76) ng/L,HBO조위(4.37 ±0.68) ng/L,각조비교차이유통계학의의(P＜0.01)；치료제14천시,대조조혈청포도당전운단백농도위(7.06 ±0.54) ng/L,모형조위(3.99 ±0.85) ng/L,이갑쌍고조위(5.06±0.77)ng/L,대조조여모형조、이갑쌍고조비교차이유통계학의의(P＜0.01).결론 HBO가능통과제고포도당전운단백수평、개선이도B세포결구여공능이기도강저혈당적작용.
Objective To observe the effect of hyperbaric oxygen (HBO) on the reduction of glucose in Goto-Kakizaki (GK) rats with type Ⅱ diabetes mellitus,and to further explore its mechanism involved.Methods Seventy-two 5-month SPF male GK rats were randomly assigned to 3 groups:the model group,the metformin hydrochloride group and the HBO group,with each group further divided into 3 subgroups.Another 24 male healthy 5-month Wistar rats were chosen as the normal control group.The rats in the HBO group received HBO treatment by inhaling pure oxygen under a constant pressure of 0.15 MPa for 30 minutes,while the rats in group 2 were treated with gastric feeding of metformin hydrochloride 250 mg/( kg · d) once a day.At the same time,all the rats in the control group,the model group and the HBO group had gastric feeding of purified water 5 ml/(kg · d) once a day.The levels of fasting blood glucose and glucose transport protein ( GLUT-1 ) of all the rats were measured respectively after 1,2 and 3 weeks of treatment.Moreover,one Wistar rat in the control group and two in the HBO group were chosen to have dynamic glucose monitoring for 72 hours.Glucose level of all the animals in the 4 groups were measured 4 hours after fasting,every other day,and 2 rats were chosen from each group for observation of the changes in the ultrastructure of islet B cells in the pancreas under the electron microscope.Results Blood glucose level of the GK rats in the metformin hydrochloride group on day 7 was (9.71 ± 2.07 )mmol/L,that of the rats in the HBO group on day 7 was(9.79 ± 2.12) mmol/L,that of the rats in the model group on day 7 was (9.99 ± 2.31 ) mmol/L,and that of the rats in control group was (4.90 ± 0.56) mmol/L.No statistical significance could be noted,when comparisons were made between the groups (P ＞ 0.05 ).Following treatment on d 7,the level of serum glucose transport protein of the control group was (7.12 ± 2.11 )ng/L,that of the model group was (3.98 ± 1.25 )ng/L,that of the metformin hydrochloride group was (4.56 ± 1.76) ng/L and that of the HBO group was (4.37 ±0.68)ng/L.Significant statistical differences could be noted,when comparisons were made between the groups(P ＜ 0.01 ).Following treatment on day 14,the level of serum glucose transport protein for the control group was (7.06 ± 0.54) ng/L,that of the model group was (3.99 ± 0.85 ) ng/L,that of the metformin hydrochloride group was (5.06 ± 0.77) ng/L.Statistical differences could be detected in the level of glucose transport protein,when comparison were made between the control group,the model group and the metformin hydrochloride group (P ＜ 0.01 ).Conclusions HBO seemed to have the capacity of lowering glucose level of GK rats through enhancement in the level of glucose transport protein and improvement of the structure/function of the islet B cells.
