中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
6期
737-738
,共2页
王楚彬%蔡培伟%伦镜盛%郭川
王楚彬%蔡培偉%倫鏡盛%郭川
왕초빈%채배위%륜경성%곽천
去甲斑蝥素%内皮细胞%毒性作用%羟苯磺酸钙%拮抗作用
去甲斑蝥素%內皮細胞%毒性作用%羥苯磺痠鈣%拮抗作用
거갑반모소%내피세포%독성작용%간분광산개%길항작용
Nor cantharidin%Endothelial cell%Toxic actions%Calcium dobesilate%Antagonism
目的 探讨羟苯磺酸钙对去甲斑蝥素致血管内皮细胞损伤的影响,并测定羟苯磺酸钙的有效拮抗作用浓度.方法 将体外培养人脐静脉内皮细胞系完全随机分为对照组、毒性组和干预组.对照组用DMEM培养基培养48 h；毒性组用含去甲斑蝥素浓度为50μmol/L的DEME培养基培养48 h；干预组分为7组,分别加入含羟苯磺酸钙5、10、20、60、80、100和200 mmoL/L的DMEM培养基中再培养46h.测定细胞存活率.结果 干预组细胞存活率随着羟苯磺酸钙剂量的增加而升高,羟苯磺酸钙剂量达80 mmol/L时,细胞存活率明显升高.四甲基偶氮唑盐微量酶反应比色法测定的干预组(5 mmol/L)细胞存活率与毒性组相近,差异无统计学意义(P＞0.05),干预组(200 mmol/L)细胞存活率高于毒性组,组间比较差异有统计学意义[(69.4±3.8)％比(49.5±1.0)％,P＜0.05].结论 去甲斑瞀素抑制内皮细胞增殖,降低细胞存活率,当羟苯磺酸钙的浓度为80 mmol/L时,对去甲斑蝥素的毒性具有明显拮抗作用,能有效保护血管内皮细胞.
目的 探討羥苯磺痠鈣對去甲斑蝥素緻血管內皮細胞損傷的影響,併測定羥苯磺痠鈣的有效拮抗作用濃度.方法 將體外培養人臍靜脈內皮細胞繫完全隨機分為對照組、毒性組和榦預組.對照組用DMEM培養基培養48 h；毒性組用含去甲斑蝥素濃度為50μmol/L的DEME培養基培養48 h；榦預組分為7組,分彆加入含羥苯磺痠鈣5、10、20、60、80、100和200 mmoL/L的DMEM培養基中再培養46h.測定細胞存活率.結果 榦預組細胞存活率隨著羥苯磺痠鈣劑量的增加而升高,羥苯磺痠鈣劑量達80 mmol/L時,細胞存活率明顯升高.四甲基偶氮唑鹽微量酶反應比色法測定的榦預組(5 mmol/L)細胞存活率與毒性組相近,差異無統計學意義(P＞0.05),榦預組(200 mmol/L)細胞存活率高于毒性組,組間比較差異有統計學意義[(69.4±3.8)％比(49.5±1.0)％,P＜0.05].結論 去甲斑瞀素抑製內皮細胞增殖,降低細胞存活率,噹羥苯磺痠鈣的濃度為80 mmol/L時,對去甲斑蝥素的毒性具有明顯拮抗作用,能有效保護血管內皮細胞.
목적 탐토간분광산개대거갑반모소치혈관내피세포손상적영향,병측정간분광산개적유효길항작용농도.방법 장체외배양인제정맥내피세포계완전수궤분위대조조、독성조화간예조.대조조용DMEM배양기배양48 h；독성조용함거갑반모소농도위50μmol/L적DEME배양기배양48 h；간예조분위7조,분별가입함간분광산개5、10、20、60、80、100화200 mmoL/L적DMEM배양기중재배양46h.측정세포존활솔.결과 간예조세포존활솔수착간분광산개제량적증가이승고,간분광산개제량체80 mmol/L시,세포존활솔명현승고.사갑기우담서염미량매반응비색법측정적간예조(5 mmol/L)세포존활솔여독성조상근,차이무통계학의의(P＞0.05),간예조(200 mmol/L)세포존활솔고우독성조,조간비교차이유통계학의의[(69.4±3.8)％비(49.5±1.0)％,P＜0.05].결론 거갑반무소억제내피세포증식,강저세포존활솔,당간분광산개적농도위80 mmol/L시,대거갑반모소적독성구유명현길항작용,능유효보호혈관내피세포.
Objective To investigate the antagonism of calcium dobesilate in vascular endothelial cells (VEC) injury by Nor cantharidin and to determine effective concentration of calcium dobesilate in the antagonism.Methods Human Umbilical Vein Endothelial Cells (HUVEC) were cultured in vitro and randomly divided into three groups:Control group,Toxic group,and Intervention groups.The 3-( 4,5-dimethylthiazol-2-yl) -2,5-dipheny-ltetrazolium bromide (MTT) assay and Trypan Blue staining were applied to detect the living cells percentage.Results Four methyl azo thiazole blue (MTT) assay showed the living cells percentage of the Intervention groups arised from (48.3 ± 2.0) ％ to ( 69.4 ± 3.8 ) ％,while the living cells percentage of intervention group with 80 mmol/L calcium dobesilate was obviously higher than that of the toxic group.Conclusions Nor cantharidin can effectively inhibit the proliferation of VEC and lower the living cells percentage of the cells.While the calcium dobesilate with a concentration of 80 mmol/L shows an antagonism of the toxicity of Nor cantharidin as well as an effect of protecting the VEC.
