CAJ | 학술논문

目的观察延胡索碱预处理对大鼠心肌是否有缩小心肌梗死范围的干预作用.方法将清洁级成年SD大鼠30只随机分为延胡索碱高、中、低剂量预处理组和经典缺血预处理组、模型组、假手术组6组(n=5),冠状动脉原位结扎,经主动脉逆行灌注2％伊文蓝,分离非缺血区和缺血区及梗死区和未梗死区,比较6组大鼠心肌缺血面积和心肌梗死面积.结果 ①心肌缺血面积:假手术组未见心肌缺血发生;与模型组比较,经典预处理组和延胡索碱中剂量组、高剂量组心肌缺血面积均有减少[(44.6±5.7)％、(46.4±7.2)％、(45.7±5.7)％比(50.4±3.3)％],差异均有统计学意义(P＜0.05或P＜0.01);与经典预处理组比较,延胡索低剂量组心肌缺血面积大[(48.9±4.4)％比(44.6±5.7)％],差异有统计学意义(P＜0.05),中剂量组、高剂量组差异无统计学意义(P＞0.05).②心肌梗死面积:假手术组未见心肌梗死发生.与模型组比较,经典预处理组和延胡索碱高、中、低剂量组梗死面积均缩小[(8.9±3.4)％、(8.5±1.8)％、( 10.1±2.7)％、(14.2±5.2)％比(50.4±3.3)％],差异均有统计学意义(均P＜0.01);与经典预处理组比较,延胡索碱低剂量组心肌梗死面积较大[(14.2±5.2)％比(8.9±3.4)％,P＜0.01],高、中剂量组心肌梗死面积与其相当,差异无统计学意义(P＞0.05).结论延胡索碱预处理对缺血再灌注心肌的保护效应与缺血预处理极为相似,这一保护效应的作用机制很可能类似于缺血预处理,即延胡索碱可能模拟缺血预处理,激活机体内源性保护机制,产生预处理效应.
목적 관찰연호색감예처리대대서심기시부유축소심기경사범위적간예작용.방법 장청길급성년SD대서30지수궤분위연호색감고、중、저제량예처리조화경전결혈예처리조、모형조、가수술조6조(n=5),관상동맥원위결찰,경주동맥역행관주2％이문람,분리비결혈구화결혈구급경사구화미경사구,비교6조대서심기결혈면적화심기경사면적.결과 ①심기결혈면적:가수술조미견심기결혈발생;여모형조비교,경전예처리조화연호색감중제량조、고제량조심기결혈면적균유감소[(44.6±5.7)％、(46.4±7.2)％、(45.7±5.7)％비(50.4±3.3)％],차이균유통계학의의(P＜0.05혹P＜0.01);여경전예처리조비교,연호색저제량조심기결혈면적대[(48.9±4.4)％비(44.6±5.7)％],차이유통계학의의(P＜0.05),중제량조、고제량조차이무통계학의의(P＞0.05).②심기경사면적:가수술조미견심기경사발생.여모형조비교,경전예처리조화연호색감고、중、저제량조경사면적균축소[(8.9±3.4)％、(8.5±1.8)％、( 10.1±2.7)％、(14.2±5.2)％비(50.4±3.3)％],차이균유통계학의의(균P＜0.01);여경전예처리조비교,연호색감저제량조심기경사면적교대[(14.2±5.2)％비(8.9±3.4)％,P＜0.01],고、중제량조심기경사면적여기상당,차이무통계학의의(P＞0.05).결론 연호색감예처리대결혈재관주심기적보호효응여결혈예처리겁위상사,저일보호효응적작용궤제흔가능유사우결혈예처리,즉연호색감가능모의결혈예처리,격활궤체내원성보호궤제,산생예처리효응.
Objective To observe whether corydalis alkaloid preprocessing can narrow myocardial infarction range of rat's myocardium.Methods All 30 adult rats with clean level were randomly divided into six groups(high,middle and low doses of corydalis alkaloid groups,classical ischemia pre-processing group,model group and sham operation group,n =5 each group).The heart was separated from body,and was ligated and retrogradely poured into 2％ Evan's blue through aorta,and then myocardial non-ischemic area and ischemic area,infracted area and ischemia non-infracted area were separated.The myocardial ischemia area and myocardial infarction area were compared among six groups.Results ①There was no myocardial ischemia in the sham operation group.Compared with the model group,myocardial ischemia area in classical ischemia pre-processing group,and middle,high doses of corydalis alkaloid groups all reduced [(44.6±5.7),(46.4±7.2),(45.7±5.7)％ vs(50.4±3.3)％ ](P ＜0.01or P ＜ 0.05).Compared with classical isehemia pre-processing group,there were no significant differences(P ＞0.05) on myocardial ischemia area in the high and middle doses groups except the low dose of corydalis alkaloid group[(48.9±4.4)％ vs(44.6±5.7)％,P＜0.05].②There was no myocardial infarction in sham operation group.Compared with the model group,the infarction size of both classical ischemia pre-processing group and high,middle and low doses levels of corydalis alkaloid groups reduced [(8.9±3.4),(8.5±1.8)(10.1±2.7),(14.2±5.2) ％ vs(50.4±3.3) ％ ](all P ＜ 0.01).Compared with the classical ischemia pre-processing group,there was no significant difference on myocardial infarct size in high and middle doses groups except the low dose of corydalis alkaloid group[(14.2±5.2)％ vs(8.9±3.4)％](P＜0.01).Conclusions There are great similarities between the protection effect of corydalis alkaloid pre-processing and ischemia preconditioning.Corydalis alkaloid may simulate ischemia preconditioning,motivate endogenous protection system and produce the pre-processing effect.