CAJ | 학술논문

目的研究高压氧(HBO)预处理诱导脊髓神经元氧化耐受的机制.方法取胚胎小鼠脊髓行原代神经元培养,分为正常对照组和HBO预处理组(HBO组).利用Westem Blot、RT-PCR等方法,观察HBO预处理对脊髓神经元抗氧化酶水平的影响.结果 HBO预处理前后超氧化物歧化酶(SOD)表达无统计学差异,血红素氧合酶(HO-1)在处理后表达增多.行RT-PCR检测HBO处理前后HO-1 mRAN水平,发现在预处理后4 h开始明显增多,处理后8 h表达达到高峰,直至处理后24 h其表达量仍比预处理前高.结论 HBO预处理对脊髓神经元氧化损伤的保护作用可能与诱导HO-1表达有关.
목적 연구고압양(HBO)예처리유도척수신경원양화내수적궤제.방법 취배태소서척수행원대신경원배양,분위정상대조조화HBO예처리조(HBO조).이용Westem Blot、RT-PCR등방법,관찰HBO예처리대척수신경원항양화매수평적영향.결과 HBO예처리전후초양화물기화매(SOD)표체무통계학차이,혈홍소양합매(HO-1)재처리후표체증다.행RT-PCR검측HBO처리전후HO-1 mRAN수평,발현재예처리후4 h개시명현증다,처리후8 h표체체도고봉,직지처리후24 h기표체량잉비예처리전고.결론 HBO예처리대척수신경원양화손상적보호작용가능여유도HO-1표체유관.
Objective To investigate mechanism of hyperbaric oxygen (HBO) preconditioninginduced tolerance to oxidative injury in primary cultured spinal cord neurons.Methods Spinal cord was obtained from fetal mice.Primary cultured neurons were divided into two groups:the control group and the HBO preconditioning group.Western Blot and reverse transcriptase polymerase chain reaction (RT-PCR) were used to observe levels of antioxidant enzyme in primary cultured spinal cord neurons.Results obtained from Western Blot showed that the level of antioxidant enzyme,heme oxygenase-1 (HO-1) in spinal cord neurons increased evidently after a single HBO exposure,reached peak at hour 8,and its expression 24 hours after HBO exposure was still higher than that before preconditioning.However,no significant differences could be noted in the expression of SOD between groups with or without HBO treatments.Conclusions The protective effect of HBO on oxidative injury in primary cultured spinal cord neurons might be involved in the expression of HO-1.