中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
CHINESE JOURNAL OF RADIATION ONCOLOGY
2011年
2期
168-171
,共4页
过氧化物酶增殖体活化受体-γ配体%纤溶酶原抑制物-1基因%转化生长因子-β基因%L929细胞%照射
過氧化物酶增殖體活化受體-γ配體%纖溶酶原抑製物-1基因%轉化生長因子-β基因%L929細胞%照射
과양화물매증식체활화수체-γ배체%섬용매원억제물-1기인%전화생장인자-β기인%L929세포%조사
Peroxisome proliferator activated receptor-γligand%Plasminogen activator inhibitor-1 gene%Transforming growth factor-βgene%L929 cell%Irradiation
目的 分析过氧化物酶增殖体活化受体-γ(PPAR-γ)配体(匹格列酮)对X射线诱导纤溶酶原抑制物-1(PAI-1)、转化生长因子-β(TGF-β)在小鼠肺成纤维细胞(L929细胞)中的mRNA表达和L929细胞增殖活性的影响.方法 用RT-PCR证实L929细胞表达PPAR-γmRNA.分别采用10、4、2 GyX射线照射L929细胞,观察照射前后PAI-1、TGF-βmRNA的表达情况.照射+匹格列酮处理L929细胞,用RT-PCR方法观察对PAI-1、TGF-βmRNA的表达影响,用MTT法观察对细胞增殖活性影响.结果 RT-PCR检测结果显示10 Gy照射48 h后L929细胞中PAI-1、TGF-βmRNA表达明显升高[0.18∶0.78、0.22∶0.76(F=3.70,P=0.010)],2 Gy和4 Gy有类似趋势[0.18∶0.43、0.18∶0.44和0.22∶0.39、0.22∶0.40(F=3.40,P=0.090)];10 Gy照射+匹格列酮48 h后明显降低[0.78∶0.45、0.76∶0.54(F=3.90,P=0.010)],2 Gy和4 Gy的也有类似趋势[0.43∶0.37、0.44∶0.35和0.39∶0.32、0.40∶0.32(F=2.40,P=0.210)].MTT结果显示与空白对照比较2、4、10 Gy照射和单用匹格列酮均会抑制L929细胞增殖[0.44∶0.39、0.36、0.34和0.32(F=3.90,P=0.040)],2、4、10 Gy照射+匹格列酮更能抑制L929细胞增殖[0.44∶0.27、0.26、0.25(F=2.50,P=0.005)].结论 X射线照射L929细胞后出现促纤维化因子PM-1、TGF-β表达升高.匹格列酮使PAI-1、TGF-β高表达降低,也会抑制成纤维细胞的增殖.
目的 分析過氧化物酶增殖體活化受體-γ(PPAR-γ)配體(匹格列酮)對X射線誘導纖溶酶原抑製物-1(PAI-1)、轉化生長因子-β(TGF-β)在小鼠肺成纖維細胞(L929細胞)中的mRNA錶達和L929細胞增殖活性的影響.方法 用RT-PCR證實L929細胞錶達PPAR-γmRNA.分彆採用10、4、2 GyX射線照射L929細胞,觀察照射前後PAI-1、TGF-βmRNA的錶達情況.照射+匹格列酮處理L929細胞,用RT-PCR方法觀察對PAI-1、TGF-βmRNA的錶達影響,用MTT法觀察對細胞增殖活性影響.結果 RT-PCR檢測結果顯示10 Gy照射48 h後L929細胞中PAI-1、TGF-βmRNA錶達明顯升高[0.18∶0.78、0.22∶0.76(F=3.70,P=0.010)],2 Gy和4 Gy有類似趨勢[0.18∶0.43、0.18∶0.44和0.22∶0.39、0.22∶0.40(F=3.40,P=0.090)];10 Gy照射+匹格列酮48 h後明顯降低[0.78∶0.45、0.76∶0.54(F=3.90,P=0.010)],2 Gy和4 Gy的也有類似趨勢[0.43∶0.37、0.44∶0.35和0.39∶0.32、0.40∶0.32(F=2.40,P=0.210)].MTT結果顯示與空白對照比較2、4、10 Gy照射和單用匹格列酮均會抑製L929細胞增殖[0.44∶0.39、0.36、0.34和0.32(F=3.90,P=0.040)],2、4、10 Gy照射+匹格列酮更能抑製L929細胞增殖[0.44∶0.27、0.26、0.25(F=2.50,P=0.005)].結論 X射線照射L929細胞後齣現促纖維化因子PM-1、TGF-β錶達升高.匹格列酮使PAI-1、TGF-β高錶達降低,也會抑製成纖維細胞的增殖.
목적 분석과양화물매증식체활화수체-γ(PPAR-γ)배체(필격렬동)대X사선유도섬용매원억제물-1(PAI-1)、전화생장인자-β(TGF-β)재소서폐성섬유세포(L929세포)중적mRNA표체화L929세포증식활성적영향.방법 용RT-PCR증실L929세포표체PPAR-γmRNA.분별채용10、4、2 GyX사선조사L929세포,관찰조사전후PAI-1、TGF-βmRNA적표체정황.조사+필격렬동처리L929세포,용RT-PCR방법관찰대PAI-1、TGF-βmRNA적표체영향,용MTT법관찰대세포증식활성영향.결과 RT-PCR검측결과현시10 Gy조사48 h후L929세포중PAI-1、TGF-βmRNA표체명현승고[0.18∶0.78、0.22∶0.76(F=3.70,P=0.010)],2 Gy화4 Gy유유사추세[0.18∶0.43、0.18∶0.44화0.22∶0.39、0.22∶0.40(F=3.40,P=0.090)];10 Gy조사+필격렬동48 h후명현강저[0.78∶0.45、0.76∶0.54(F=3.90,P=0.010)],2 Gy화4 Gy적야유유사추세[0.43∶0.37、0.44∶0.35화0.39∶0.32、0.40∶0.32(F=2.40,P=0.210)].MTT결과현시여공백대조비교2、4、10 Gy조사화단용필격렬동균회억제L929세포증식[0.44∶0.39、0.36、0.34화0.32(F=3.90,P=0.040)],2、4、10 Gy조사+필격렬동경능억제L929세포증식[0.44∶0.27、0.26、0.25(F=2.50,P=0.005)].결론 X사선조사L929세포후출현촉섬유화인자PM-1、TGF-β표체승고.필격렬동사PAI-1、TGF-β고표체강저,야회억제성섬유세포적증식.
Objective To observe the influence of peroxisome proliferator activated receptor-γligand (PPAR-γ, pioglatazone) on expression of PAI-1 and TGF-β mRNA and proliferation in fibroblast cells before and after X-ray radiation, and to study the effect of PPAR-γon normal cells during radiation induced fibrosis process. Methods RT-PCR method was used to measure PPAR-γgene expression in L929 cells.After X-ray irradiation of 10 Gy,4 Gy or 2 Gy, the expressions of PAI-1 and TGF-β mRNA in mouse lung fibroblast cells (L929) were measured using RT-PCR. After X-ray irradiation and pioglatazone treatment,the influence of pioglatazone on PAI-1 and TGF-β was measured using RT-PCR method. MTT method was used to test cell proliferation after the treatment of irradiation and pioglatazone. Results PPAR-γ mRNA expression was observed in L929 cells. Expression of PAI-1 and TGF-β mRNA reached the highest level 483.40,P =0. 090) ). At 48 h after the treatment of pioglatazone and 10 Gy radiation, pioglatazone decreased 0. 36, 0. 34 and 0. 32( F = 3.90, P = 0. 040) ). The inhibitory effect was significantly increased when L9292. 50,P =0. 005)). Conclusions X-ray irradiation can increase the expression of PAI-1 and TGF-β in L929 cells. Pioglatazone can decrease the expression of radiation-induced PAI-1 and TGF-β, and restrain the fibroblast proliferation.
