中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
3期
218-221
,共4页
涂文强%孙秀敏%秦丽娜%时燕薇%成建定%赵虎
塗文彊%孫秀敏%秦麗娜%時燕薇%成建定%趙虎
도문강%손수민%진려나%시연미%성건정%조호
母爱剥夺%海马%神经发育%PP1Cγ
母愛剝奪%海馬%神經髮育%PP1Cγ
모애박탈%해마%신경발육%PP1Cγ
Maternal deprivation%Hippocampus%Neurobehavior%PP1Cγ
目的 探讨母爱剥夺(MD)对PP1Cγ的表达改变和神经发育的影响.方法 新生雄性仔鼠随机分为MD组(35只)和正常对照组(24只),MD组在出生后1～21 d每天接受3h的MD.通过早期神经行为实验观察神经发育情况,水迷宫实验观察学习记忆的影响,实时荧光定量PCR分析PP1Cγ的基因表达改变.结果 MD能改变大鼠体质量、上肢运动协调能力和大鼠痛觉敏感性[(26.23±2.81)g,(30.38±3.85)g;(19.37±11.89)s,(22.39±17.62)s;(4.36±1.76)s,(5.26±2.55)s;P＜0.05],对神经反射影响较小[(0.83±0.30)s,(0.83±0.34)s;(3.68±1.63)s,(5.61±3.01)s;(3.00±0.00),(3.00±0.00);P＞0.05],对学习记忆能力影响不明显(P＞0.05).在出生后第22天MD可致海马PP1Cγ表达下降[(2.19±0.62),(3.52±0.86);P＜0.05],在出生后60d大鼠PP1Cγ表达无明显改变[(1.73±0.78),(1.33±0.34);P＞0.05],而在出生后第90天海马PP1Cγ表达升高[(2.85±0.34),(1.34±0.93);P＜0.05].结论 MD可致大鼠早期神经行为和海马PP1Cγ的表达发生改变,但对学习记忆的影响却很小.同时PP1Cγ表达随生长发育时期的不同而改变.
目的 探討母愛剝奪(MD)對PP1Cγ的錶達改變和神經髮育的影響.方法 新生雄性仔鼠隨機分為MD組(35隻)和正常對照組(24隻),MD組在齣生後1～21 d每天接受3h的MD.通過早期神經行為實驗觀察神經髮育情況,水迷宮實驗觀察學習記憶的影響,實時熒光定量PCR分析PP1Cγ的基因錶達改變.結果 MD能改變大鼠體質量、上肢運動協調能力和大鼠痛覺敏感性[(26.23±2.81)g,(30.38±3.85)g;(19.37±11.89)s,(22.39±17.62)s;(4.36±1.76)s,(5.26±2.55)s;P＜0.05],對神經反射影響較小[(0.83±0.30)s,(0.83±0.34)s;(3.68±1.63)s,(5.61±3.01)s;(3.00±0.00),(3.00±0.00);P＞0.05],對學習記憶能力影響不明顯(P＞0.05).在齣生後第22天MD可緻海馬PP1Cγ錶達下降[(2.19±0.62),(3.52±0.86);P＜0.05],在齣生後60d大鼠PP1Cγ錶達無明顯改變[(1.73±0.78),(1.33±0.34);P＞0.05],而在齣生後第90天海馬PP1Cγ錶達升高[(2.85±0.34),(1.34±0.93);P＜0.05].結論 MD可緻大鼠早期神經行為和海馬PP1Cγ的錶達髮生改變,但對學習記憶的影響卻很小.同時PP1Cγ錶達隨生長髮育時期的不同而改變.
목적 탐토모애박탈(MD)대PP1Cγ적표체개변화신경발육적영향.방법 신생웅성자서수궤분위MD조(35지)화정상대조조(24지),MD조재출생후1～21 d매천접수3h적MD.통과조기신경행위실험관찰신경발육정황,수미궁실험관찰학습기억적영향,실시형광정량PCR분석PP1Cγ적기인표체개변.결과 MD능개변대서체질량、상지운동협조능력화대서통각민감성[(26.23±2.81)g,(30.38±3.85)g;(19.37±11.89)s,(22.39±17.62)s;(4.36±1.76)s,(5.26±2.55)s;P＜0.05],대신경반사영향교소[(0.83±0.30)s,(0.83±0.34)s;(3.68±1.63)s,(5.61±3.01)s;(3.00±0.00),(3.00±0.00);P＞0.05],대학습기억능력영향불명현(P＞0.05).재출생후제22천MD가치해마PP1Cγ표체하강[(2.19±0.62),(3.52±0.86);P＜0.05],재출생후60d대서PP1Cγ표체무명현개변[(1.73±0.78),(1.33±0.34);P＞0.05],이재출생후제90천해마PP1Cγ표체승고[(2.85±0.34),(1.34±0.93);P＜0.05].결론 MD가치대서조기신경행위화해마PP1Cγ적표체발생개변,단대학습기억적영향각흔소.동시PP1Cγ표체수생장발육시기적불동이개변.
Objective To investigate the effect of maternal deprivation (MD) on neurobehavior and PP1Cγgene expression in hippocampus. Methods Male pups were randomly divided into MD group(thirty-five)and control group(twenty-four). From PND 1 to PND 21 ,pups in the MD groups underwent daily maternal deprivation for 3 h ( Postnatal day). Neurobehavior was observed to investigate neurodevelopment, Morris water maze was used to measure spatial learning and memory,and Real-Time quantitative PCR was employed to analyze PP1Cγ gene expression. Results Several significant deficiencies were observed in bodyweight and grasping reflex while a great enhancement in hot-plate test in rat pups suffering from MD( (26.23 ± 2.81 )g vs. (30. 38 ± 3.85 )g;( 19.37 ± 11.89) s vs. (22.39 ± 17.62 ) s; (4.36 ± 1.76 ) s vs. ( 5.26 ± 2.55 ) s; P ＜ 0. 05 ), but deficiencies in neurological reflexes were subtle ( ( 0.83 ±- 0.30 ) s vs. ( 0. 83 ± 0. 34 ) s; ( 3.68 ± 1.63 ) s vs. ( 5.61 ± 3. 01 ) s;( 3.00 ± 0.00 ) vs. ( 3.00 ± 0. 00); P ＞ 0. 05 ). MD had a subtle influence on spatial learning and memory (P ＞0.05). Meanwhile,MD could lead to PP1Cγ expression down-regulation on PND 22 ( (2.19 ±0.62) vs. (3.52 ±0.86), P＜0. 05)which was in line with early neurobehavior results. No difference was found compared with MD group and control group on PND60 ( ( 1.73 ± 0. 78 ) vs. ( 1.33 ± 0. 34); P ＞ 0.05 ). However, there was the up-regulation of PP1Cγexpression on PND 90 ( (2.85 ± 0. 34) vs. ( 1.34 ± 0.93 ); P ＜ 0.05 ). Conclusion MD alters early neurobehavior and hippocampal PP1Cγgene expression in the Wistar rats,but has a subtle effect on learning and memory. At the same time,MD can make PP1Cγexpression in the hippocampus varying with the age.
