CAJ | 학술논문

目的了解云南省人群中流行的乙型肝炎病毒(Hepatitis B virus,HBV)基因型和血清型分布情况.方法从参加健康体检的人群中筛查HBsAg阳性的血清标本,利用巢式PCR扩增HBV S基因片段,并对扩增产物进行序列测定.从GenBank中查获A～I基因型共27株HBV参考序列,构建HBV S基因系统发生树,以此确定其样本的基因型及血清型,并对结果进行统计分析.结果从2216名体检人员血清标本中筛查出 39份HBsAg阳性的样本,HBV的S基因序列分型结果表明有4种基因型:C型占76.9%,B型占15.4%;D型占5.1%;I型占2.5%.血清分型结果为:adw2型占71.8%;adrq+型占17.9%;ayt型占10.3%.所有adw2血清型标本均为C基因型.HBsAg、HBeAg双阳性标本中75%为C基因型/adw2血清亚型.结论云南省HBV感染人群中HBV基因型的分布以C型为主,血清型以adw2型为主.
목적 료해운남성인군중류행적을형간염병독(Hepatitis B virus,HBV)기인형화혈청형분포정황.방법 종삼가건강체검적인군중사사HBsAg양성적혈청표본,이용소식PCR확증HBV S기인편단,병대확증산물진행서렬측정.종GenBank중사획A～I기인형공27주HBV삼고서렬,구건HBV S기인계통발생수,이차학정기양본적기인형급혈청형,병대결과진행통계분석.결과 종2216명체검인원혈청표본중사사출 39빈HBsAg양성적양본,HBV적S기인서렬분형결과표명유4충기인형:C형점76.9%,B형점15.4%;D형점5.1%;I형점2.5%.혈청분형결과위:adw2형점71.8%;adrq+형점17.9%;ayt형점10.3%.소유adw2혈청형표본균위C기인형.HBsAg、HBeAg쌍양성표본중75%위C기인형/adw2혈청아형.결론 운남성HBV감염인군중HBV기인형적분포이C형위주,혈청형이adw2형위주.
Objective To know genotypes and serotypes of hepatitis B virus ( HBV ) detected from hepatitis B infected people in Yunnan Province. Methods Serum samples were collected from HBsAg carriers detected from people who had a physical examination at Yunnan Provincial Center for Disease Control and Prevention. The S genes of HBV were amplified by nested PCR and the PCR products were sequenced.The viral genotype was identified by phylogenetic analysis. 27 reference sequences corresponding to HBV genotype A to I were obtained from GenBank. According to the amino acid sequences deduced from the nucleotide sequences of S gene, the dominant serotype of HBV detected from these people were confirmed.Results 39 HBsAg positive serum samples were detected from 2216 people who had a physical examination. The results shows that 76.9% were C genotype; 15.4% were B genotype;5.1% were D genotype;2. 5% were I genotype. Three serotypes were found. The rates of adw2, adrq+ and ayr serotypes are 71.8% ,17.9% and 10. 3% respectively. All of adw2 subtype specimens are C genotype. Among the serum specimens in which both HBsAg and HBeAg are positive, 75% were C genotype and adw2 subtype.Conclusion It is determined that the main genotype and subtype of HBV prevailed in Yunnan province is C genotype and adw2 subtype.