CAJ | 학술논문

目的探讨凋亡相关基因在骨肉瘤细胞中的表达及其临床意义.方法采用3个公认的骨肉瘤细胞株及1个成骨细胞株,提取总RNA,合成生物素标记的cRNA与Affymetrix(R)GeneChip(R) U133A芯片杂交,筛选差异表达的凋亡相关基因.利用MATLAB软件进行细胞凋亡通路的KEGG分析.结果以表达差异≥2.0倍为限,在Affymetrix(R)HG-U133A芯片包含的所有基因中,3个骨肉瘤细胞株与成骨细胞株比较,一共筛选出7个差异表达的凋亡相关基因:3个上调基因(IKBKB、IL1R1和FAS),4个下调基因(TP53、PPP3CB、PPP3CC和APAF1).利用MATLAB软件将7个差异表达的凋亡相关基因映射到KEGG的凋亡通路上.结论骨肉瘤足细胞增殖和细胞凋亡间平衡失调的结果,通过调控细胞凋亡相关基因的表达可以诱导肿瘤细胞凋亡,达到抗肿瘤目的 .
목적 탐토조망상관기인재골육류세포중적표체급기림상의의.방법 채용3개공인적골육류세포주급1개성골세포주,제취총RNA,합성생물소표기적cRNA여Affymetrix(R)GeneChip(R) U133A심편잡교,사선차이표체적조망상관기인.이용MATLAB연건진행세포조망통로적KEGG분석.결과 이표체차이≥2.0배위한,재Affymetrix(R)HG-U133A심편포함적소유기인중,3개골육류세포주여성골세포주비교,일공사선출7개차이표체적조망상관기인:3개상조기인(IKBKB、IL1R1화FAS),4개하조기인(TP53、PPP3CB、PPP3CC화APAF1).이용MATLAB연건장7개차이표체적조망상관기인영사도KEGG적조망통로상.결론 골육류족세포증식화세포조망간평형실조적결과,통과조공세포조망상관기인적표체가이유도종류세포조망,체도항종류목적 .
Objective To explore the expression of apoptosis related genes in osteosarcoma cells,and study their clinical significance in osteoflarcoma.Methods Three well-known ATCC osteosarcoma cell lines and an osteoblastic cell line were collected.After the total RNA extractionmthe finally synthesized biotinylated cRNAS were hybridized to Affymetrix(R) GeneChip(R) U133A arrays.Bioinformatics method was used to screen the apoptosis related genes which were differentially expressed.Results Using an entrance limit of≥2.0,there were 3 up-regulated(IKBKB,IL1R1 and FAS)and 4 dowm-regulated apoptosis related genes(TP53,PPP3CB,PPP3CC and APAF1)identified by mieroarray which were commonly differentially expressed among all the three osteosarcoma cell lines in comparison with the osteoblastie cell line hfobl.19.Conclusion Osteosarcoma iS a result of disequilibrium between cell proliferation and apoptosis.Regulating the expression of apoptosis related genes may induce apoptosis of tumor cells.and achieve the purpose of anti-tumor.