CAJ | 학술논문

目的对~(18)F-FHBG体外摄取、体内分布及荷瘤裸鼠PET-CT显像等方面进行研究.方法利用γ井型计数器测定体外肿瘤细胞T47D和T47D-tk对~(18)F-FHBG的摄取、正常昆明小鼠和移植瘤裸鼠~(18)F-FHBG体内分布;并进行荷皮下移植瘤裸鼠~(18)F-FHBG PET-CT显像.结果体外肿瘤细胞摄取实验显示T47D-tk细胞摄取~(18)F-FHBG的程度明显高于正常的T47D细胞,120 min时T47D-tk细胞对~(18)F-FHBG的摄取为T47D细胞的64倍(P<0.001).正常小鼠注射~(18)F-FHBG后,主要分布于肝脏、肠道、肾脏和膀胱,而脑部未见明显放射性分布.荷皮下移植瘤裸鼠PET-CT显像显示,T47D-tk肿瘤浓聚~(18)F-FHBG的程度明显高于T47D肿瘤,且2 h显像效果较好.结论体外T47D-tk细胞摄取~(18)F-FHBG的程度明显高于正常的T47D细胞;在小鼠体内~(18)F-FHBG主要经肠道和泌尿系统排泄.~(18)F-FHBG报告基因探针可以有效的定位于HSV1-tk基因表达的部位且与移植瘤裸鼠体内分布研究结果一致.这可为进一步开展~(18)F-FHBG报告基因显像与肿瘤基因治疗的监测提供有效手段和科学依据.
목적 대~(18)F-FHBG체외섭취、체내분포급하류라서PET-CT현상등방면진행연구.방법 이용γ정형계수기측정체외종류세포T47D화T47D-tk대~(18)F-FHBG적섭취、정상곤명소서화이식류라서~(18)F-FHBG체내분포;병진행하피하이식류라서~(18)F-FHBG PET-CT현상.결과 체외종류세포섭취실험현시T47D-tk세포섭취~(18)F-FHBG적정도명현고우정상적T47D세포,120 min시T47D-tk세포대~(18)F-FHBG적섭취위T47D세포적64배(P<0.001).정상소서주사~(18)F-FHBG후,주요분포우간장、장도、신장화방광,이뇌부미견명현방사성분포.하피하이식류라서PET-CT현상현시,T47D-tk종류농취~(18)F-FHBG적정도명현고우T47D종류,차2 h현상효과교호.결론 체외T47D-tk세포섭취~(18)F-FHBG적정도명현고우정상적T47D세포;재소서체내~(18)F-FHBG주요경장도화비뇨계통배설.~(18)F-FHBG보고기인탐침가이유효적정위우HSV1-tk기인표체적부위차여이식류라서체내분포연구결과일치.저가위진일보개전~(18)F-FHBG보고기인현상여종류기인치료적감측제공유효수단화과학의거.
Objective To study the in vitro accumulation of ~(18)F-FHBG, its in vivo distribution and ~(18)F-FHBG PET-CT imaging for reporter gene (HSVl-tk) in nude mice with a xenograft of breast adenocarcinoma Methods The in vitro uptake of ~(18)F-FHBG in tumor cells of T47D and T47D-tk and the distribution of ~(18)F-FHBG in normal Kunming mice and nude mice with breast adenocarcinoma xenograft were detected by well-type γ counter. Reporter gene PET-CT imaging with ~(18)F-FHBG was performed in nude mice with a xenograft of breast adenocarcinoma. And the expression location of HSVl-tk gene could be monitored by observing the in vitro and in vivo accumulation of ~(18)F-FHBG. Results The in vitro uptake of ~(18)F-FHBG in T47D-tk cells (143. 67 dpm/10~4 ± 5. 82 dpm/10~4 cells) was significantly higher than that in T47D cells (2.23 dpm/10~4 ± 0.23 dpm/10~4 cells) at 60 and 120 min post-injection ( P < 0. 001 ) and reaches a plateau at 60 min. In normal Kunming mice, ~(l8)F-FHBG was mainly distributed in liver, intestine, kidney and bladder while there was no obvious radioactive accumulation in brain. F-FHBG accumulated at a significantly higher level in T47D-tk tumors than in T47D tumors and its accumulation yielded the best image effect at 2 h by PET-CT imaging in nude mice. Conclusion The in vitro uptake of ~(18)F-FHBG in T47D-tk cells is significantly higher than that in T47D cells. ~(18)F-FHBG is mainly excreted by digestive tract and urinary tract in mice. It agrees with the expression pattern of HSVl-tk gene. ~(18)F-FHBG can determine the localization of HSVl-tk gene expression in an efficient way. This study will offer a monitoring method and scientific base for ~(18)F-FHBG reporter gene imaging and HSV1 -tk gene therapy in tumors.