CAJ | 학술논문

迄今,有关个体的疼痛程度和炎症程度之间的精确关系一直存在争论,主要原因是缺乏能够同时反映多种痛(尤其是可鉴别疼痛即早期和持续期)的炎症模型以及定量方法的合理应用.因此,本研究在啮齿类动物评价了外周皮下组织致炎后炎症水肿与伤害性反应以及痛敏之间的相关性.为了更好地认识炎症特异性特征在治疗中的价值,我们对非甾体类抗炎药的作用效果也进行了评价.将一个剂量的蜜蜂毒(0.05 mg/0.025 ml)注入12个近交系(129P3/J、A/J、AKR/J、BALB/cJ、C3H/HeJ、C57BL/6J、C57BL/10J、C58/J、CBA/J、DBA/2J、RIIIS/J和SM/J)小鼠或6个剂量的蜜蜂毒(0.001、0.005、0.01、0.05、0.1、0.2 mg/0.05 ml)注入远交系(Sprague-Dawley)大鼠的一侧足底皮下,分别检测自发伤害性反应、热和机械性痛敏,以及炎症的水肿和局部皮温,然后对组间和组内数据进行相关性分析.此外,观察非甾体类抗炎药吲哚美辛对痛和炎症的作用效果.结果显示:(1)炎症水肿程度与注射侧自发缩足反射次数、舔足抬足时间等伤害性反射程度呈高度正相关(P≤0.003),而与热或机械性痛敏的程度没有相关性;(2)吲哚美辛(0.5、2.5、25 mg/kg,i.p.,稀释于60%二甲基桠枫)可以剂量依赖性地抑制炎症水肿和自发伤害性反应,但是对热或机械性痛敏却只有在最高剂量下才有作用.这些结果提示,炎症水肿过程可能只参与动物受炎症刺激而引起的即早自发伤害性反应过程,而不参与与临床更加密切相关的痛敏过程.这个分析结果为确定抗炎治疗有益于缓解多种炎性痛中的哪个靶表证提供了一个有用的分析方法.
흘금,유관개체적동통정도화염증정도지간적정학관계일직존재쟁론,주요원인시결핍능구동시반영다충통(우기시가감별동통즉조기화지속기)적염증모형이급정량방법적합리응용.인차,본연구재교치류동물평개료외주피하조직치염후염증수종여상해성반응이급통민지간적상관성.위료경호지인식염증특이성특정재치료중적개치,아문대비치체류항염약적작용효과야진행료평개.장일개제량적밀봉독(0.05 mg/0.025 ml)주입12개근교계(129P3/J、A/J、AKR/J、BALB/cJ、C3H/HeJ、C57BL/6J、C57BL/10J、C58/J、CBA/J、DBA/2J、RIIIS/J화SM/J)소서혹6개제량적밀봉독(0.001、0.005、0.01、0.05、0.1、0.2 mg/0.05 ml)주입원교계(Sprague-Dawley)대서적일측족저피하,분별검측자발상해성반응、열화궤계성통민,이급염증적수종화국부피온,연후대조간화조내수거진행상관성분석.차외,관찰비치체류항염약신타미신대통화염증적작용효과.결과현시:(1)염증수종정도여주사측자발축족반사차수、첨족태족시간등상해성반사정도정고도정상관(P≤0.003),이여열혹궤계성통민적정도몰유상관성;(2)신타미신(0.5、2.5、25 mg/kg,i.p.,희석우60%이갑기아풍)가이제량의뢰성지억제염증수종화자발상해성반응,단시대열혹궤계성통민각지유재최고제량하재유작용.저사결과제시,염증수종과정가능지삼여동물수염증자격이인기적즉조자발상해성반응과정,이불삼여여림상경가밀절상관적통민과정.저개분석결과위학정항염치료유익우완해다충염성통중적나개파표증제공료일개유용적분석방법.
The preciserelationship between the degree of pain and the degree of inflammation in the individual remains debated. A quantitative analysis simultaneously applied to the immediate and prolonged painful consequences of inflammation has not yet been done. Thus, the correlations between edema, nociception and hypersensitivity following an inflammatory insult were assessed in rodents. To better understand the therapeutic value of modifying specific aspects of inflammation, the effects of an anti-inflammatory drug were compared to the results. Inbred strains of mice and outbred rats received an intraplantar injection of honeybee venom and the between-group and within-group correlations were calculated for spontaneous nociceptive measures, thermal and mechanical hypersensitivity, and edema and temperature. The effect of indomethacin on the pain and inflammation measures was examined.Edema correlated with spontaneous flinching, licking and lifting of the injected paw (P ≤ 0.003), and not with thermal or mechanical hypersensitivity. Indomethacin affected edema and spontaneous nociception dose-dependently, and affected hypersensitivity only at the highest dose tested (P ＜ 0.05). These results suggest that edemamay contribute only to immediate spontaneous nociceptive responses to an inflammatory insult, and not to the more clinically relevant prolonged hypersensitivity. This analysis represents a method for determining which inflammatory processes are the most promising therapeutic targets against the multiple painful consequences of inflammation.