食品科学
食品科學
식품과학
FOOD SCIENCE
2009年
15期
120-123
,共4页
卢惠妮%潘迎捷%孙晓红%赵勇
盧惠妮%潘迎捷%孫曉紅%趙勇
로혜니%반영첩%손효홍%조용
棘托竹荪%子实体%抑菌作用%最低抑菌浓度
棘託竹蓀%子實體%抑菌作用%最低抑菌濃度
극탁죽손%자실체%억균작용%최저억균농도
Dicryophora echinovolvata%fruitbody%bacteriostatic effect%minimum inhibitory concentration (MIC)
以水为提取剂,对棘托竹荪子实体进行浸提,利用琼脂甲板打孔法测定棘托竹荪子实体对大肠杆菌O157:H7、副溶血弧菌、单增李斯特菌、肠炎沙门氏菌和金黄色葡萄球菌的抑菌效果,并研究其对5种病原菌的最低抑菌浓度(MIC)、热稳定性以及抑菌pH值范围.结果表明,棘托竹荪子实体的浸提液对副溶血弧菌抑制作用最好,其次是单增李斯特菌、大肠杆菌O157,对肠炎沙门氏菌、金黄色葡萄球菌抑制效果最差;棘托竹荪子实体的浸提液最低抑菌浓度小,抑菌pH值范围广而且热稳定性强.
以水為提取劑,對棘託竹蓀子實體進行浸提,利用瓊脂甲闆打孔法測定棘託竹蓀子實體對大腸桿菌O157:H7、副溶血弧菌、單增李斯特菌、腸炎沙門氏菌和金黃色葡萄毬菌的抑菌效果,併研究其對5種病原菌的最低抑菌濃度(MIC)、熱穩定性以及抑菌pH值範圍.結果錶明,棘託竹蓀子實體的浸提液對副溶血弧菌抑製作用最好,其次是單增李斯特菌、大腸桿菌O157,對腸炎沙門氏菌、金黃色葡萄毬菌抑製效果最差;棘託竹蓀子實體的浸提液最低抑菌濃度小,抑菌pH值範圍廣而且熱穩定性彊.
이수위제취제,대극탁죽손자실체진행침제,이용경지갑판타공법측정극탁죽손자실체대대장간균O157:H7、부용혈호균、단증리사특균、장염사문씨균화금황색포도구균적억균효과,병연구기대5충병원균적최저억균농도(MIC)、열은정성이급억균pH치범위.결과표명,극탁죽손자실체적침제액대부용혈호균억제작용최호,기차시단증리사특균、대장간균O157,대장염사문씨균、금황색포도구균억제효과최차;극탁죽손자실체적침제액최저억균농도소,억균pH치범위엄이차열은정성강.
Aqueous extract of Dictyophora echinovolvata fruitbody was prepared by boiling water extraction and its antibacterial activity against 5 species of common food-borne pathogenic bacteria was investigated by agar diffusion plate method. Additionally, the minimum inhibitory concentration (MIC) and thermal stabifity as well as antibacterial pH range of this extract were also analyzed. The extract of Dictyophora echinovolvata fruithody displayed the highest inhibitory effect against Vibrio parahaemolyticus, followed by Listeria monocytogenes and Escherichia coli O157:H7 while no significant bacteriostatic effects were observed on Salmonella enteritidis and Staphylococcus aureus. Tiffs extract possessed quite lower MIC values, wide antibacterial pH range and the strong thermal stability.