基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2010年
1期
33-36
,共4页
张丹丹%徐占民%宋爱丽%赵全明%董小黎
張丹丹%徐佔民%宋愛麗%趙全明%董小黎
장단단%서점민%송애려%조전명%동소려
~(18)F-FDG PET/CT%不稳定斑块%巨噬细胞%平滑肌细胞
~(18)F-FDG PET/CT%不穩定斑塊%巨噬細胞%平滑肌細胞
~(18)F-FDG PET/CT%불은정반괴%거서세포%평활기세포
~(18)F-FDG PET/CT%unstable plaque%macrophage%smooth muscle cell
目的 探讨~(18)氟标记的脱氧葡萄糖(~(18)F-FDG)PET/CT无创检测不稳定斑块的可行性.方法 用新西兰雄性大白兔建立动脉粥样硬化模型,注射~(18)F-FDG后行腹主动脉PET/CT成像,离体动脉数码照相,测定动脉片段的放射强度,选取不稳定和稳定斑块各30块,比较其放射强度,采用免疫组化方法观察斑块中的巨噬细胞和平滑肌细胞并计数.结果 PET/CT活体成像可见实验组沿腹主动脉走行的不均匀的放射性分布,与离体动脉的斑块分布基本一致;不稳定斑块组的靶-非靶比值和巨噬细胞数明显高于稳定斑块组(P<0.01),而平滑肌细胞数明显降低(P<0.01);靶-非靶比值与斑块中的巨噬细胞数呈正相关(r=0.815,P<0.01),而与平滑肌细胞数呈负相关(r=-0.684,P<0.01).结论 ~(18)F-FDG PET/CT无创检测实验性不稳定斑块具有一定的可行性.
目的 探討~(18)氟標記的脫氧葡萄糖(~(18)F-FDG)PET/CT無創檢測不穩定斑塊的可行性.方法 用新西蘭雄性大白兔建立動脈粥樣硬化模型,註射~(18)F-FDG後行腹主動脈PET/CT成像,離體動脈數碼照相,測定動脈片段的放射彊度,選取不穩定和穩定斑塊各30塊,比較其放射彊度,採用免疫組化方法觀察斑塊中的巨噬細胞和平滑肌細胞併計數.結果 PET/CT活體成像可見實驗組沿腹主動脈走行的不均勻的放射性分佈,與離體動脈的斑塊分佈基本一緻;不穩定斑塊組的靶-非靶比值和巨噬細胞數明顯高于穩定斑塊組(P<0.01),而平滑肌細胞數明顯降低(P<0.01);靶-非靶比值與斑塊中的巨噬細胞數呈正相關(r=0.815,P<0.01),而與平滑肌細胞數呈負相關(r=-0.684,P<0.01).結論 ~(18)F-FDG PET/CT無創檢測實驗性不穩定斑塊具有一定的可行性.
목적 탐토~(18)불표기적탈양포도당(~(18)F-FDG)PET/CT무창검측불은정반괴적가행성.방법 용신서란웅성대백토건립동맥죽양경화모형,주사~(18)F-FDG후행복주동맥PET/CT성상,리체동맥수마조상,측정동맥편단적방사강도,선취불은정화은정반괴각30괴,비교기방사강도,채용면역조화방법관찰반괴중적거서세포화평활기세포병계수.결과 PET/CT활체성상가견실험조연복주동맥주행적불균균적방사성분포,여리체동맥적반괴분포기본일치;불은정반괴조적파-비파비치화거서세포수명현고우은정반괴조(P<0.01),이평활기세포수명현강저(P<0.01);파-비파비치여반괴중적거서세포수정정상관(r=0.815,P<0.01),이여평활기세포수정부상관(r=-0.684,P<0.01).결론 ~(18)F-FDG PET/CT무창검측실험성불은정반괴구유일정적가행성.
Objective To study the feasibility of noninvasive detection of unstable plaques with ~(18)F-Fluorodeoxyglu-cose (~(18)F-FDG) PET/CT imaging. Methods Atherosclerotic plaques were induced in male New Zealand white rabbits. Animals were injected with FDG labeled with ~(18)F, then examined with PET/CT. Aorta was explanted for photography with digital camera, and ~(18)F-FDG uptake analysis. Thirty unstable plaques and 30 stable plaques were choosed so as to compare the quantitativly ~(18)F-FDG uptake. The number of macrophages and smooth muscle cells was detected by immunohistochemical technique. Results Experimental group showed inconsistent uptake of ~(18)F-FDG in the abdominal aorta. The results were confirmed in the ex vivo digital photo of the explanted aorta. The target to non target ratio (T/NT) and macrophages of unstable plaques were higher than stable plaques (P<0.01) , but smooth muscle cells obviously reduced (P <0. 01). Correlation analysis showed that there was a positive correlation between T/NT and macrophage content (r=0. 815,P<0. 01), and a negative correlation between T/NT and SMC content(r=-0. 684,P <0. 01). Conclusion ~(18)F-FDG PET/CT can constitute an attractive imaging method for the noninvasive detection of experimental unstable plaques.
