CAJ | 학술논문

[目的]研究不同杀菌剂对莲瓣兰杂交种子原生地共生萌发突破种皮的胚培养过程中真菌污染控制及胚培养成活情况的影响.[方法]以莲瓣兰杂交种子原生地共生萌发突破种皮的胚为外植体,分别用1.0 g/L代森锰锌、1.0 g/L多菌灵、1.0 g/L甲霜灵和1.0 mg/L培福朗预处理外植体5 min,再按常规灭菌后将外植体接种到改良RM培养基中在适宜条件下培养,观察胚培养过程中真菌污染控制及胚培养成活情况.[结果]莲瓣兰杂交种子原生地共生萌发突破种皮的胚经1.0 g/L代森锰锌和1.0 mg/L培福朗预处理5 min,胚培养过程中真菌污染率控制在10.0%以内,显著优于无处理的对照组(CK);代森锰锌处理胚培养成活率较低,胚培养成活率仅为67.5%,培福朗处理胚培养成活率显著高于代森锰锌,胚培养成活率高达84.8%.[结论]培福朗是莲瓣兰胚培养理想的杀菌剂.
[목적]연구불동살균제대련판란잡교충자원생지공생맹발돌파충피적배배양과정중진균오염공제급배배양성활정황적영향.[방법]이련판란잡교충자원생지공생맹발돌파충피적배위외식체,분별용1.0 g/L대삼맹자、1.0 g/L다균령、1.0 g/L갑상령화1.0 mg/L배복랑예처리외식체5 min,재안상규멸균후장외식체접충도개량RM배양기중재괄의조건하배양,관찰배배양과정중진균오염공제급배배양성활정황.[결과]련판란잡교충자원생지공생맹발돌파충피적배경1.0 g/L대삼맹자화1.0 mg/L배복랑예처리5 min,배배양과정중진균오염솔공제재10.0%이내,현저우우무처리적대조조(CK);대삼맹자처리배배양성활솔교저,배배양성활솔부위67.5%,배복랑처리배배양성활솔현저고우대삼맹자,배배양성활솔고체84.8%.[결론]배복랑시련판란배배양이상적살균제.
[Objective] The research aimed to study the effects of different fungicides on the fungus contamination control and the embryo culture survival in the process of symbiotic germination embryo cultuture of hybrid seeds of Cymbidium tortisepalum in situ. [Method] Using the symbiotic germination embryo hybrid seeds of Cymbidium tortisepalum in situ as explants, the explants were pretreated with 1.0 g/ml Mancozeb, 1.0 g/ml carbendazim, 1.0 g/ml etalaxyl and 1.0 g/ml uazatine for 5 min respectively. After conventional sterilization, the explants were inoculated in improved RM culture medium and cultured under suitable conditions to observe the fungus contamination control and embryo survival ratio in the course of embryo culture. [Result] The fungus contamination percent of embryo form of Cymbidium tortisepalum's hybrid seeds in situ symbiotic germination was controlled within 10% by using 1.0 g/L Mancozeb and 1.0 mg/L uazatine for 5 minuts, significantly superior to control group. The survival ratio of embryo culture with Mancozeb was lower, only 67.5%. The survival ratio of embryo culture with uazatine was remarkably higher than Mancozeb and the survival ratio of embryo culture was 84.8%. [Conclusion] Uazatine was the perfect fungicide of Cymbidium tortisepalum's embryo culture.