CAJ | 학술논문

采用浸泡发芽培养方法改变水稻分子内部构象,测定其变性淀粉果胶酶活力的变化,选择最佳浸泡发芽条件并测定变性后淀粉灰度.结果表明:最佳培养条件为浸泡温度25℃,浸泡方式为浸泡4 h断水10 h,加碱量为0.03%Ca(OH)2,培养时间为2.5 d,在25℃条件下发芽6 d,变性淀粉果胶酶活性最高,灰度较好.从而成功实现对发芽水稻变性淀粉果胶酶活性及其灰度分布的测定.
채용침포발아배양방법개변수도분자내부구상,측정기변성정분과효매활력적변화,선택최가침포발아조건병측정변성후정분회도.결과표명:최가배양조건위침포온도25℃,침포방식위침포4 h단수10 h,가감량위0.03%Ca(OH)2,배양시간위2.5 d,재25℃조건하발아6 d,변성정분과효매활성최고,회도교호.종이성공실현대발아수도변성정분과효매활성급기회도분포적측정.
By soaking the rice for the cultivation the molecular conformation changed,and determinated the change of the pectinase activity,then determinated the best immersion and germination conditions and the gray-scale.The results showed that the best conditions for cultivate was that immersion temperature is 25℃,immersion mode is 4 hours immersion and 10 hours un-immersion,immersion alkali content is 0.03% Ca(OH)2, immersion time is 2.5 days, germination temperature is 25℃,germination time is 6 days, the pectinase activity and the gray-scale of the modified starch were best at this time.The pectinase activity and the gray-scale of rice modified starch were determinated successfully.