CAJ | 학술논문

用SDS-PAGE技术研究了不同时期条斑紫菜样品、高温下培养的丝状体以及不同季节收获的叶状体的总可溶性蛋白.结果表明,这些样品总可溶性蛋白的表达数量和种类相差很大.壳孢子中总可溶性蛋白的表达数量和种类明显比丝状体中多,但比膨大丝状体中少;高温条件下培养的丝状体比正常条件下培养的丝状体表达更多数量和种类的可溶性蛋白;所有样品中,以叶状体表达的溶性蛋白的表达数量和种类最多;上年收获的与本年度不同季节收获的叶状体在蛋白质表达图谱上差别很小.
용SDS-PAGE기술연구료불동시기조반자채양품、고온하배양적사상체이급불동계절수획적협상체적총가용성단백.결과표명,저사양품총가용성단백적표체수량화충류상차흔대.각포자중총가용성단백적표체수량화충류명현비사상체중다,단비팽대사상체중소;고온조건하배양적사상체비정상조건하배양적사상체표체경다수량화충류적가용성단백;소유양품중,이협상체표체적용성단백적표체수량화충류최다;상년수획적여본년도불동계절수획적협상체재단백질표체도보상차별흔소.
Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.