CAJ | 학술논문

目的观察神经干细胞、雪旺细胞和组织工程材料乙交酯-丙交酯共聚物共移植后对大鼠损伤脊髓形态和功能的修复作用.方法 36只Wistar大鼠,随机数字法分为乙交酯-丙交酯共聚物移植组、神经干细胞/乙交酯-丙交酯共聚物绀和神经干细胞+雪旺细胞/乙交酯-丙交酯共聚物组.体外培养、鉴定胚胎脊髓源神经干细胞和雪旺细胞,制备和构建乙交酯-丙交酯共聚物支架细胞复合体并移植到大鼠脊髓T9半横断损伤部位,应用BBB行为评分和电生理技术在术后4、12周评价大鼠脊髓功能的恢复情况;应用透射电镜、HE染色和免疫组织化学染色方法在形态结构上观察轴突和髓鞘再生情况,以及神经干细胞在脊髓内的存活、迁移和分化情况.结果术后4、12周,细胞移植组的BBB评分较对照组明显提高(P＜0.05);细胞移植组的体感诱发电位和运动诱发电位波幅较对照组都有所好转.术后12周移植材料正中横断面透射电镜可见新生的无髓及有髓神经纤维;脊髓标本免疫组织化学染色显示移植的神经十细胞呵以在宿主脊髓内存活、迁移并分化成神经元和少枝胶质细胞,未分化成星形胶质细胞.结论神经干细胞、雪旺细胞和组织工程材料乙交酯-丙交酯共聚物共移植可以促进半横断损伤的大鼠脊髓轴突再生,改善肢体的运动功能.
목적 관찰신경간세포、설왕세포화조직공정재료을교지-병교지공취물공이식후대대서손상척수형태화공능적수복작용.방법 36지Wistar대서,수궤수자법분위을교지-병교지공취물이식조、신경간세포/을교지-병교지공취물감화신경간세포+설왕세포/을교지-병교지공취물조.체외배양、감정배태척수원신경간세포화설왕세포,제비화구건을교지-병교지공취물지가세포복합체병이식도대서척수T9반횡단손상부위,응용BBB행위평분화전생리기술재술후4、12주평개대서척수공능적회복정황;응용투사전경、HE염색화면역조직화학염색방법재형태결구상관찰축돌화수초재생정황,이급신경간세포재척수내적존활、천이화분화정황.결과술후4、12주,세포이식조적BBB평분교대조조명현제고(P＜0.05);세포이식조적체감유발전위화운동유발전위파폭교대조조도유소호전.술후12주이식재료정중횡단면투사전경가견신생적무수급유수신경섬유;척수표본면역조직화학염색현시이식적신경십세포가이재숙주척수내존활、천이병분화성신경원화소지효질세포,미분화성성형효질세포.결론 신경간세포、설왕세포화조직공정재료을교지-병교지공취물공이식가이촉진반횡단손상적대서척수축돌재생,개선지체적운동공능.
Objective To observe the morphological and functional repairing effects of co-transplantation of neural stem cell (NSC), Schwann cell (SC) and a polylactide-co-glycolide acid (PLGA) scaffold on the spinal cord injury (SCI) in rats. Methods Thirty-six wistar rats were divided into 3 equal groups randomly according to the transplantation materials: PLGA (A), NSC/PLGA (B)and NSC+SC/PLGA(C).The complex of PLGA scaffold/cells Was constructed and implanted into the injury site of T9 in SCI models developed from wistar rats. BBB locomotion scores, motor evoked potentials (MEP) and somatosensory evoked potentials (SEP) of lower limbs were examined to learn the rehabilitation of sensory and motor funotions at 4 and 12 weeks after injury in difierent groups. Al the SCI tissues were observed with transelectronmicroscopy. HE and immunohistochemistry to identify the regeneration of neural fibers and the survival, migration and differentiation of the NSC at 4 and 12 weeks after injury. Results From 4 to 12 weeks, the BBB locomotion scores in all the transplantation groups increased, especially in the CO-transplantation groups(P＜0.05).The amplitudes of SEP and MEP at 4 weeks became lower than at 12 weeks after injury in groups B and C, but they were not observed at 4 and 12 weeks after injury in group A. The regenerated axons were observed in the scaffold/cells complex with transelectronmicroseopy. Immunohistochemistry staining showed the transplanted NSCs could survive. Migrate and difierentiate into neuron and oligodendrocyte at 12 weeks. Conclusion Co-transplantation of NSC+SC/PLGA can promote the functional recovery of the injured spinal cord.