CAJ | 학술논문

目的评价异氟醚预处理和异丙酚预处理对大鼠心肌缺血再灌注损伤的影响.方法雄性Wistar大鼠36只,体重250～300 g,随机分为4组(n=9):缺血再灌注组(I/R组)、异氟醚预处理组(Ⅰ组)、异丙酚预处理组(P组)和异氟醚预处理联合异丙酚预处理组(I+P组).Ⅰ组异氟醚预处理方法:吸入1.6%异氟醚10 min,停止吸入5 min,共重复2次;P组异丙酚预处理方法:静脉输注异丙酚37.5 mg·kg~(-1)·h~(-1) 10 min,停止输注5 min,共重复2次;I+P组同时进行异氟醚预处理和异丙酚预处理.预处理后立即结扎左冠状动脉前降支60 min,随后松开进行再灌注,I/R组只进行缺血再灌注.再灌注120 min时每组取1只大鼠,取心肌组织,透射电镜下观察心肌细胞超微结构;各组其余大鼠处死后,取左心室,采用TUNEL法测定心肌细胞凋亡情况,计算凋亡指数,并测定心肌细胞线粒体活性氧(ROS)水平.结果各组均可见凋亡小体,I组、P组和I+P组心肌损伤程度轻于I/R组.与I/R组比较,I组、P组和I+P组心肌细胞凋亡指数和ROS水平降低(P＜0.05),而I组、P组和I+P组间上述指标比较差异无统计学意义(P＞0.05).结论异氟醚预处理或异丙酚预处理及两种方法联合应用时减轻大鼠心肌缺血再灌注损伤的效应相似.
목적 평개이불미예처리화이병분예처리대대서심기결혈재관주손상적영향.방법 웅성Wistar대서36지,체중250～300 g,수궤분위4조(n=9):결혈재관주조(I/R조)、이불미예처리조(Ⅰ조)、이병분예처리조(P조)화이불미예처리연합이병분예처리조(I+P조).Ⅰ조이불미예처리방법:흡입1.6%이불미10 min,정지흡입5 min,공중복2차;P조이병분예처리방법:정맥수주이병분37.5 mg·kg~(-1)·h~(-1) 10 min,정지수주5 min,공중복2차;I+P조동시진행이불미예처리화이병분예처리.예처리후립즉결찰좌관상동맥전강지60 min,수후송개진행재관주,I/R조지진행결혈재관주.재관주120 min시매조취1지대서,취심기조직,투사전경하관찰심기세포초미결구;각조기여대서처사후,취좌심실,채용TUNEL법측정심기세포조망정황,계산조망지수,병측정심기세포선립체활성양(ROS)수평.결과 각조균가견조망소체,I조、P조화I+P조심기손상정도경우I/R조.여I/R조비교,I조、P조화I+P조심기세포조망지수화ROS수평강저(P＜0.05),이I조、P조화I+P조간상술지표비교차이무통계학의의(P＞0.05).결론 이불미예처리혹이병분예처리급량충방법연합응용시감경대서심기결혈재관주손상적효응상사.
Objective To evaluate the effects of preconditioning with isoflurane and propofol on myocardial ischemia-reperfusion(I/R)injury in rots.Methods Thirty-six male Wistar rats weighing 250-300 g were randomly divided into 4 groups(n=9 each)： group Ⅰ I/R; group Ⅱ isoflurane preconditioning(group Ⅰ);group Ⅲ propofol preconditioning(group P)and group Ⅳ preconditioning with isoflurane and propofol(group I+P).The animals were anesthetized with intraperitoneal urethane.The trachea was intubated.The animals were mechanically ventilated.In group Ⅱ and Ⅳ the animals received 2 episodes of 10 min 1.6% isoflurane inhalation at 5 min intervals before I/R; in group Ⅲ and Ⅳ the animals received 2 episodes of 10 rain iv propefol infusion at 37.5 mg·kg~(-1)·h~(-1) at 5 min intervals before I/R.Preconditioning with isoflurane and propefol was performed simultaneously in group I+P(Ⅳ).Myocardial I/R was produced by 60 min occlusion of left anterior descending coronary artery followed by 120 min reperfusion.The animals were sacrificed at the end of 120 min reperfusion and the hearts were removed for microscopic examination with transmission electron microscope，detection of myocyte apoptosis by TUNEL staining and determination of mitochondrial ROS level by fluorescence staining.Results Cardiomyocyte apoptosis was detected by transmission electron microscopy.Apoptosis index and mitochondrial ROS level were significantly lower in group I，group P and group I+P than in group I/R，but no difference was found among the 3 preconditioning groups.Conclusion The protection provided by preconditioning with isoflurane or propofol or isoflurane+propofol against myocardial I/R is comparable.