CAJ | 학술논문

目的研究鞘内注射星形胶质细胞抑制剂氟代柠檬酸(fluorocitrate,FC)和/或小胶质细胞特异性抑制剂米诺四环素(minocycline,MI)对C3H/He小鼠跟骨癌性疼痛及肿瘤的影响.方法将雄性C3H/He小鼠按配伍组设计随机法分为6组(n=20),包括:正常组、假手术组、癌痛+人工脑脊液组(artificial cerebrospinal fluid,ACSF)、癌痛+FC组、癌痛+MI组、癌痛+FC+MI组.术后每天给药1次,持续21 d,并于术前当天、术后3、5、7、10、14、21 d采用机械性痛觉过敏行为学及冷痛敏测定小鼠行为学变化,天平秤称量小鼠体重变化及组织切片HE染色观察肿瘤骨的骨质破坏程度.结果术前当天各组小鼠的疼痛刺激缩足反应差异无统计学意义(P＞0.05);与正常组及基础值相比,术后3 d假手术组小鼠疼痛刺激缩足反应显著变化(P＞0.05);而癌痛组小鼠疼痛刺激缩足反应显著增加(P＜0.05).其中癌痛组内,与ACSF组相比,术后5 d MI组小鼠疼痛刺激缩足反应显著降低(P＜0.05);MI+FC组小鼠刺激性缩足反应也显著降低(P＜0.01);而FC组术后10 d小鼠刺激性缩足反应显著降低(P＜0.05).术前当天各组组间基础体重差异无统计学意义(P＞0.05).与正常组相比,假手术组和癌痛组小鼠的体重在术后3 d虽有所下降,但差异无统计学意义(P＞0.05).与正常组相比,术后21 d癌痛组小鼠体重显著降低(P＜0.05);与ACSF组相比,MI组、FC组及MI+FC组体重降低较少(P<0.05).同时间点内,癌痛各组间组织切片HE染色发现肿瘤的大小及骨的破坏程度差异无统计学意义.结论鞘内同时注射氟代柠檬酸和/或米诺四环素明显降低癌痛小鼠的缩足反应,抑制其痛觉过敏或痛觉超敏的产生,改善骨癌痛小鼠体重的变化,但对肿瘤本身的生长及组织的破坏程度无影响.
목적 연구초내주사성형효질세포억제제불대저몽산(fluorocitrate,FC)화/혹소효질세포특이성억제제미낙사배소(minocycline,MI)대C3H/He소서근골암성동통급종류적영향.방법 장웅성C3H/He소서안배오조설계수궤법분위6조(n=20),포괄:정상조、가수술조、암통+인공뇌척액조(artificial cerebrospinal fluid,ACSF)、암통+FC조、암통+MI조、암통+FC+MI조.술후매천급약1차,지속21 d,병우술전당천、술후3、5、7、10、14、21 d채용궤계성통각과민행위학급랭통민측정소서행위학변화,천평칭칭량소서체중변화급조직절편HE염색관찰종류골적골질파배정도.결과 술전당천각조소서적동통자격축족반응차이무통계학의의(P＞0.05);여정상조급기출치상비,술후3 d가수술조소서동통자격축족반응현저변화(P＞0.05);이암통조소서동통자격축족반응현저증가(P＜0.05).기중암통조내,여ACSF조상비,술후5 d MI조소서동통자격축족반응현저강저(P＜0.05);MI+FC조소서자격성축족반응야현저강저(P＜0.01);이FC조술후10 d소서자격성축족반응현저강저(P＜0.05).술전당천각조조간기출체중차이무통계학의의(P＞0.05).여정상조상비,가수술조화암통조소서적체중재술후3 d수유소하강,단차이무통계학의의(P＞0.05).여정상조상비,술후21 d암통조소서체중현저강저(P＜0.05);여ACSF조상비,MI조、FC조급MI+FC조체중강저교소(P<0.05).동시간점내,암통각조간조직절편HE염색발현종류적대소급골적파배정도차이무통계학의의.결론 초내동시주사불대저몽산화/혹미낙사배소명현강저암통소서적축족반응,억제기통각과민혹통각초민적산생,개선골암통소서체중적변화,단대종류본신적생장급조직적파배정도무영향.
Objective To study the influences of intrathecal injection of astrocytes inhibitor fluorocitrate (FC) and/or microglia inhibitor minocycline (MI) on on tumour growth and pain in bone cancer mouse model. Methods C3H/He mice suffering from bone cancer were intrathecally injected with nothing, artificial CSF, FC, MI, or FC+MI. Behaviour, body weight, bone tumor biopsy was observed or detected. Results Results On the preoperative day, paw withdrawal response of mice in each group was no significant different (P＞0.05); compared with the normal group and the baseline, on the 3rd day after operation, the paw withdrawal response of mice in sham-operated group was no significant changed (P＞0.05); compared with normal group, on the 3rd day after operation, the paw withdrawal response was significantly increased(P＜0.05 ). And compared with ACSF group, on the 5th day after operation, the paw withdrawal response ofmic in the group FC+MI, was significantly decreased (P＜0.01), and was also significantly decreased in the group MI (P＜0.05 ). On the 10th day after operation, the paw withdrawal response was significantly decreased in the group FC(P＜0.05 ). On the preoperative day, the basis of body weight in each group was no significant different (P＞0.05). Compared with normal group, the body weight of mice on the 3rd day after operation in sham-operated group and the cancer pain groups reduced somewhat, but there were no statistical significances (P＞0.05). Compared with normal group, body weight of mice on the 21th day after operation in the cancer pain groups decreased significantly (P＜0.05); compared with the ACSF group, MI group, FC group and MI + FC group, the body weight of mice was less reduced, there were statistical significances (P＜0.05). At the same time point, compared with each other, there was no significance within the cancer pain groups, through the tumor size and bone mass of the extent of the damage. Conclusion Intrathecal fluorocitrate and/or minocycline injection can increase the pain threshold, thereby inhibiting hyperalgesia and pain high-sensitivity, without influence on tumor growth and bone damage extent in bone cancer mouse model.