CAJ | 학술논문

目的探讨SD大鼠结核杆菌感染模型的建立方法,比较不同感染途径对动物模型的影响.方法 60只SD大鼠随机分成4组,使用标准人型H37Rv结核杆菌菌株0.01 mg,分别从右侧胸腔、腹腔、尾静脉、尾部皮内注入,注菌8周后处死各组大鼠,观察大鼠肺、肝、脾大体病变及病理改变,注射部位迟发超敏反应情况,取肺组织匀浆作抗酸染色涂片并培养观察有无结核杆菌生长.结果 HE染色下肺、肝、脾可见不同程度的结核性炎症改变,肺组织匀浆抗酸染色可见不同数量的短小、红染分枝杆菌.胸腔、尾静脉注菌组胸部脏器病理改变较腹腔、尾部皮内注菌组改变典型,组织匀浆培养阳性率高,腹腔注菌组腹腔肝、脾结核改变较其余组明显.结论 SD大鼠对结核杆菌敏感,胸腔、腹腔、尾静脉注入结核杆菌均可建立大鼠结核杆菌感染模型,另外,胸腔、腹腔、尾静脉途径分别适用于建立结核性胸膜炎、腹腔结核、肺结核模型,尾部皮内注射途径一般仅应用于制造免疫.
목적 탐토SD대서결핵간균감염모형적건립방법,비교불동감염도경대동물모형적영향.방법 60지SD대서수궤분성4조,사용표준인형H37Rv결핵간균균주0.01 mg,분별종우측흉강、복강、미정맥、미부피내주입,주균8주후처사각조대서,관찰대서폐、간、비대체병변급병리개변,주사부위지발초민반응정황,취폐조직균장작항산염색도편병배양관찰유무결핵간균생장.결과 HE염색하폐、간、비가견불동정도적결핵성염증개변,폐조직균장항산염색가견불동수량적단소、홍염분지간균.흉강、미정맥주균조흉부장기병리개변교복강、미부피내주균조개변전형,조직균장배양양성솔고,복강주균조복강간、비결핵개변교기여조명현.결론 SD대서대결핵간균민감,흉강、복강、미정맥주입결핵간균균가건립대서결핵간균감염모형,령외,흉강、복강、미정맥도경분별괄용우건립결핵성흉막염、복강결핵、폐결핵모형,미부피내주사도경일반부응용우제조면역.
Objective To develop SD rat model of Mycobacterium tuberculosis infection and to compare different routes of infection in animal model. Methods 60 SD rats were randomly divided into four groups. The rats were respectively injected with 0.01 mg standard strain of Mycobacterium tuberculosis H37Rv through the right side of the chest, abdomen, tail vein, and the tail skin. At the eighth week after injection, the rats were killed to observe the gross lesions and pathological changes of lung, liver, spleen and delayed hypersensitivity at the injection site. The lung tissue smear was used to observe the growth of Mycobacterium tuberculosis by acid-fast staining and cultivation. Results The lung, liver,and spleen showed different degrees of tuberculous inflammatory changes by HE staining. The lung tissue showed different number of short, red dye Mycobacterium by acid-fast staining. Compared with groups of injection in abdomen and tail skin,the pathological changes of chest were more typical and the positive rate of tissue culture was higher in groups of injection in chest and tail vein. The tuberculosis changes of liver and spleen in group of intraperitoneal injection were more significant than those in other groups. Conclusions SD rats are sensitive to Mycobacterium tuberculosis. The injection of Mycobacterium tuberculosis by chest, abdomen,and tail vein can establish the rat model of Mycobacterium tuberculosis infection. In addition, the injection of Mycobacterium tuberculosis by chest, abdomen, and tail vein are respectively fit for the establishment of tuberculous pleurisy, abdominal tuberculosis, and tuberculosis model, the injection in tail skin is generally only used to produce immunity.