中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2009年
8期
484-486
,共3页
徐妍%叶俊%朱可建%程浩%朱宁%陈贤祯
徐妍%葉俊%硃可建%程浩%硃寧%陳賢禎
서연%협준%주가건%정호%주저%진현정
尖锐湿疣%抗原,CD4%T淋巴细胞%受体,白细胞介素%转录因子%免疫,细胞
尖銳濕疣%抗原,CD4%T淋巴細胞%受體,白細胞介素%轉錄因子%免疫,細胞
첨예습우%항원,CD4%T림파세포%수체,백세포개소%전록인자%면역,세포
Condyloma acuminata%Antigens,CD4%T-lyrnphocytes%Receptors,interleukin%Transcription factors%Immunity,cellular
目的 了解尖锐湿疣患者外周血Foxp3+CD4+CD25+调节性T细胞的表达水平,探讨其在尖锐湿疣发生发展机制中的作用.方法 分别收集30例尖锐湿疣患者(复发15例,初发15例)及20例健康者外周抗凝静脉血,分离出外周血单个核细胞.藻红蛋白(PE)标记抗CD4单抗,异硫氰酸荧光素(FITC)标记抗CD25单抗,细胞破膜后PE标记的抗Foxp3单抗行细胞内染色,三色流式细胞术分析Foxp3+CD4+CD25+调节性T细胞比例.组间比较采用ANOVA检验.结果外周血中Foxp3+CD4+CD25+调节性T细胞水平在尖锐湿疣患者组为(3.4±1.0)%,在复发组为(4.7±1.2)%,均显著高于健康对照组的(1.2±0.5)%(P<0.01).外周血Foxp3+CD4+CD25+调节性T细胞水平在初发组为(2.1±1.0)%,高于健康对照组,但差异无统计学意义,而在复发组明显高于初发组(P<0.05).结论 尖锐湿疣患者外周血Foxp3+CD4<"+>CD25+调节性T细胞数量增加,这种细胞免疫功能失调可能与其免疫学发病机制有关.
目的 瞭解尖銳濕疣患者外週血Foxp3+CD4+CD25+調節性T細胞的錶達水平,探討其在尖銳濕疣髮生髮展機製中的作用.方法 分彆收集30例尖銳濕疣患者(複髮15例,初髮15例)及20例健康者外週抗凝靜脈血,分離齣外週血單箇覈細胞.藻紅蛋白(PE)標記抗CD4單抗,異硫氰痠熒光素(FITC)標記抗CD25單抗,細胞破膜後PE標記的抗Foxp3單抗行細胞內染色,三色流式細胞術分析Foxp3+CD4+CD25+調節性T細胞比例.組間比較採用ANOVA檢驗.結果外週血中Foxp3+CD4+CD25+調節性T細胞水平在尖銳濕疣患者組為(3.4±1.0)%,在複髮組為(4.7±1.2)%,均顯著高于健康對照組的(1.2±0.5)%(P<0.01).外週血Foxp3+CD4+CD25+調節性T細胞水平在初髮組為(2.1±1.0)%,高于健康對照組,但差異無統計學意義,而在複髮組明顯高于初髮組(P<0.05).結論 尖銳濕疣患者外週血Foxp3+CD4<"+>CD25+調節性T細胞數量增加,這種細胞免疫功能失調可能與其免疫學髮病機製有關.
목적 료해첨예습우환자외주혈Foxp3+CD4+CD25+조절성T세포적표체수평,탐토기재첨예습우발생발전궤제중적작용.방법 분별수집30례첨예습우환자(복발15례,초발15례)급20례건강자외주항응정맥혈,분리출외주혈단개핵세포.조홍단백(PE)표기항CD4단항,이류청산형광소(FITC)표기항CD25단항,세포파막후PE표기적항Foxp3단항행세포내염색,삼색류식세포술분석Foxp3+CD4+CD25+조절성T세포비례.조간비교채용ANOVA검험.결과외주혈중Foxp3+CD4+CD25+조절성T세포수평재첨예습우환자조위(3.4±1.0)%,재복발조위(4.7±1.2)%,균현저고우건강대조조적(1.2±0.5)%(P<0.01).외주혈Foxp3+CD4+CD25+조절성T세포수평재초발조위(2.1±1.0)%,고우건강대조조,단차이무통계학의의,이재복발조명현고우초발조(P<0.05).결론 첨예습우환자외주혈Foxp3+CD4<"+>CD25+조절성T세포수량증가,저충세포면역공능실조가능여기면역학발병궤제유관.
Objective To observe the levels of Foxp3+ CD+ CD25+ regulatory T cells in the peripheral blood of condyloma acuminatum (CA) patients and investigate their roles in the pathogenesis of CA. Methods The peripheral blood was collected from 30 CA patients (including 15 with relapsing and 15 with first onset) and 20 healthy controls. Peripheral blood mononuclear ceils (PBMC) were isolated and stained with anti-human CD4-PE-Cy5 and anti-human CD25-fluorescein isothiocyanate (FITC) monoclonal antibodies on cell membrane, followed by intraeellular staining with anti-human Foxp3-PE. The percentage of Foxp3+ CD4+-CD25+ regulatory T cells was detected by three-color flow cytometry. Comparison between groups was done by ANOVA test. Results The percentages of Foxp3+ CD4+ CD25+ regulatory T cells among total CD4 + T cells in CA patients and relapsing CA group were (3.4 ± 1.0) % and (4.7 ±+ 1.2) %, respectively, which were both significantly higher than that in healthy control group [(1.2±0. 5)%, P<0.01]. Furthermore, that in first onset CA group was (2. 1 ± 1.0) %, which was higher than that in healthy control group, but without statistical significance; but that in relapsing CA group was significantly higher than that in first onset group (P<0.05). Conclusions The number of Foxp3+ CD4+ CD25+ regulatory T cells increases in the peripheral blood of CA patients. The disorder of cellular immunity may be involved in the immunopathogenesis of CA.
