中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
6期
476-478
,共3页
邓小梅%刘敏%郑桂喜%王传新%张建%李伟
鄧小梅%劉敏%鄭桂喜%王傳新%張建%李偉
산소매%류민%정계희%왕전신%장건%리위
乳头状瘤病毒%人%宫颈肿瘤%宫颈上皮内瘤样病变%基因型%液相芯片
乳頭狀瘤病毒%人%宮頸腫瘤%宮頸上皮內瘤樣病變%基因型%液相芯片
유두상류병독%인%궁경종류%궁경상피내류양병변%기인형%액상심편
Popillomavims%human%Cervix neoplasms%Cervical intraepithelial neoplasia%Genotype%Liquid chip
目的 评价Luminex XMAP液相芯片技术在HPV感染检测中的临床价值.方法 取宫颈组织标本231份和正常宫颈脱落细胞33份,采用液相芯片技术和杂交捕获Ⅱ(HC Ⅱ)对所有标本进行HPV DNA检测.比较两种方法检测结果,对不一致标本及HPV单一型感染标本进行基因测序.结果 两种方法检测结果一致性极好(Kappa=0.8889).根据液相芯片检测结果,HPV检出率为82.95%,不同基因型感染率从高到低依次为:HPV 16、52、58、18、11、31、6、39、33、56、70.其中117份单一型感染,102份多重感染.高危型感染占87.43%,低危型12.57%.以组织病理学为确诊标准,液相芯片和HCⅡ检测CINⅡ、CINⅢ和宫颈癌HPV DNA的灵敏度、特异度、阳性预测值、阴性预测值分别依次为:93.07%、87.88%、98.17%、64.44%和94.81%、87.88%、98.21%、70.73%.所有液相芯片检出的单一型经基因测序验证准确率为98.29%.结论 所选宫颈病变患者HPV感染的常见型别是16、52、58、18、11、31、6.Luminex XMAP液相芯片技术是HPV分型检测及大规模筛查的理想方法.
目的 評價Luminex XMAP液相芯片技術在HPV感染檢測中的臨床價值.方法 取宮頸組織標本231份和正常宮頸脫落細胞33份,採用液相芯片技術和雜交捕穫Ⅱ(HC Ⅱ)對所有標本進行HPV DNA檢測.比較兩種方法檢測結果,對不一緻標本及HPV單一型感染標本進行基因測序.結果 兩種方法檢測結果一緻性極好(Kappa=0.8889).根據液相芯片檢測結果,HPV檢齣率為82.95%,不同基因型感染率從高到低依次為:HPV 16、52、58、18、11、31、6、39、33、56、70.其中117份單一型感染,102份多重感染.高危型感染佔87.43%,低危型12.57%.以組織病理學為確診標準,液相芯片和HCⅡ檢測CINⅡ、CINⅢ和宮頸癌HPV DNA的靈敏度、特異度、暘性預測值、陰性預測值分彆依次為:93.07%、87.88%、98.17%、64.44%和94.81%、87.88%、98.21%、70.73%.所有液相芯片檢齣的單一型經基因測序驗證準確率為98.29%.結論 所選宮頸病變患者HPV感染的常見型彆是16、52、58、18、11、31、6.Luminex XMAP液相芯片技術是HPV分型檢測及大規模篩查的理想方法.
목적 평개Luminex XMAP액상심편기술재HPV감염검측중적림상개치.방법 취궁경조직표본231빈화정상궁경탈락세포33빈,채용액상심편기술화잡교포획Ⅱ(HC Ⅱ)대소유표본진행HPV DNA검측.비교량충방법검측결과,대불일치표본급HPV단일형감염표본진행기인측서.결과 량충방법검측결과일치성겁호(Kappa=0.8889).근거액상심편검측결과,HPV검출솔위82.95%,불동기인형감염솔종고도저의차위:HPV 16、52、58、18、11、31、6、39、33、56、70.기중117빈단일형감염,102빈다중감염.고위형감염점87.43%,저위형12.57%.이조직병이학위학진표준,액상심편화HCⅡ검측CINⅡ、CINⅢ화궁경암HPV DNA적령민도、특이도、양성예측치、음성예측치분별의차위:93.07%、87.88%、98.17%、64.44%화94.81%、87.88%、98.21%、70.73%.소유액상심편검출적단일형경기인측서험증준학솔위98.29%.결론 소선궁경병변환자HPV감염적상견형별시16、52、58、18、11、31、6.Luminex XMAP액상심편기술시HPV분형검측급대규모사사적이상방법.
Objective To evaluate the diagnostic value of Luminex XMAP liquid chip for HPv detection.Methods Detect HPV DNA with the liquid chip and HC Ⅱ in 264 cases,including 231 of cervical cancer and 33of cervical scrapes.ne accordance of two methods were compared and DNA sequencing was performed in conflicting samples and single(infection samples.Results There is an excellent agreement between the methods.The overall incidence of HPV was 82.95%,the most common genotypes were HPV 16,52,58,18,11,31,6,39,33,56,70.Among all the positive types,117 were single type and 102 were multiple types,and 87.43% were high-risk HPV genotypes and 12.57% were low-risk genotypes.Based on the criteria of histology and pathology.the sensitivity,specificity,positive and negative-predictive value of HPV liquid chip and HC Ⅱ for detecting all cases of CIN Ⅱ,Ⅲand carcinomas were respectively 93.07%,87.88%,98.17%,64.44% and 94.81%,87.88%,98.21%,70.73%.Conclusion The common types of HPV infection are 16,52,58,18,11,6,56and 31.Multiplex HPV genotyping by Luminex XMAP liquid chip appears to be highly suitable for diagnostic screening and large-scale epidemiological studies.
