CAJ | 학술논문

目的探讨子宫颈鳞状细胞癌组织中SCCA1和SCCA2 m RNA的表达在子宫颈鳞状细胞癌临床诊断、疗效评价及预后观察中的作用.方法采用TaqMan探针实时聚合酶链反应(RT-PCR)的方法,分析60例子宫颈鳞状细胞癌患者和30例正常子宫颈组织中SCCA1和SCCA2 mRNA的表达.结果 SCCA2 mRNA在子宫颈鳞状细胞癌组织中较对照正常组织中表达水平高(相对表达量分别为4.405±2.310及9.088±2.195),差异有统计学意义(t=-6.513,P＜0.05).SCCA1 mRNA则差异无统计学意义(t=-0.115,P＞0.05).SCCA2mRNA的表达随着临床分期的增高而增高(F=8.313,P＜0.05),SCCA2 mRNA的表达在有淋巴结转移组较无淋巴结转移组高(t=2.853,P＜0.05),SCCA2 mRNA的表达与病理分级及患者年龄无关(P＞0.05),SCCA1 mRNA的表达与患者年龄、病理分级、临床分期及淋巴结转移均无明显相关性(P＞0.05).结论 SCCA2 mRNA的表达可能为子宫颈鳞状细胞癌临床分期、淋巴结转移的判断提供更为准确的信息.
목적 탐토자궁경린상세포암조직중SCCA1화SCCA2 m RNA적표체재자궁경린상세포암림상진단、료효평개급예후관찰중적작용.방법 채용TaqMan탐침실시취합매련반응(RT-PCR)적방법,분석60례자궁경린상세포암환자화30례정상자궁경조직중SCCA1화SCCA2 mRNA적표체.결과 SCCA2 mRNA재자궁경린상세포암조직중교대조정상조직중표체수평고(상대표체량분별위4.405±2.310급9.088±2.195),차이유통계학의의(t=-6.513,P＜0.05).SCCA1 mRNA칙차이무통계학의의(t=-0.115,P＞0.05).SCCA2mRNA적표체수착림상분기적증고이증고(F=8.313,P＜0.05),SCCA2 mRNA적표체재유림파결전이조교무림파결전이조고(t=2.853,P＜0.05),SCCA2 mRNA적표체여병리분급급환자년령무관(P＞0.05),SCCA1 mRNA적표체여환자년령、병리분급、림상분기급림파결전이균무명현상관성(P＞0.05).결론 SCCA2 mRNA적표체가능위자궁경린상세포암림상분기、림파결전이적판단제공경위준학적신식.
Objective To study the expression levels of SCCA1 and SCCA2 mRNA in tissues of cervical squamous cell carcinoma. To investigate the role of this gene in the clinical diagnosis, evaluation of treatment and observation of prognosis of cervical squamous cell carcinoma. Methods Quantitative real-time RT-PCR was used to detect the expression of SCCA1 and SCCA2 mRNA in tissues of 60 cases of cervical squamous cell carcinoma and those of 30 cases of normal cervical tissues. Results The expression level of SCCA2 mRNA in tissues of 30 cases of cervical squamous cell carcinoma was higher than in those of 15 cases of normal cervical tissues (4.405 ± 2.310, 9.088 ± 2.195) (t =-6.513, P ＜0.001), while the expression level of SCCA 1 mRNA did not significantly differ between normal and malignant tissues (P ＞0.05). The expression of SCCA2 mRNA was relevant to FIGO stages and there was a tendency for this gene to increase with the stage getting worse (F =8.313, P ＜0.05). Moreover, the overexpression of SCCA2 mRNA was significantly correlated with lymph node metastases (t =2.853, P ＜0.05). The expression of SCCA2 mRNA was not correlated with age and pathological grading (P ＞0.05). However, the expression of SCCA1 mRNA was not correlated with age,FIGO stages, lymph node metastases and histological grade (P ＞0.05). Conclusion The expression of SCCA2 mRNA may provide help for more accurate diagnosis on the clinical stages and lymph node metastases of cervical squamous cell carcinoma.